Navegação por Autores IPEN "SABINO, CAETANO P."

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  • IPEN-DOC 20840

    TENORIO, DENISE P.L.A.; ANDRADE, CAMILA G.; CABRAL FILHO, PAULO E.; SABINO, CAETANO P. ; KATO, ILKA T. ; CARVALHO JUNIOR, LUIZ B.; ALVES JUNIOR, SEVERINO; RIBEIRO, MARTHA S. ; FONTES, ADRIANA; SANTOS, BEATE S.. CdTe quantum dots conjugated to concanavalin A as potential fluorescent molecular probes for saccharides detection in Candida albicans. Journal of Photochemistry and Photobiology B: Biology, v. 142, p. 237-243, 2015.

    Palavras-Chave: candida; diagnosis; concanavalin a; fluorescence; cadmium tellurides; quantum dots; saccharides


  • IPEN-DOC 24458

    SABINO, CAETANO P. ; HAMBLIN, MICHAEL R.. Cellular damage. In: SELLERA, FABIO P. (Ed.); NASCIMENTO, CRISTIANE L. (Ed.); RIBEIRO, MARTHA S. (Ed.). Photodynamic therapy in veterinary medicine: from basics to clinical practice. Gewerbestrasse, Switzerland: Springer, 2016. p. 57-72, DOI: 10.1007/978-3-319-45007-0_5

    Observação: Livro na íntegra disponível. Consulte a biblioteca do IPEN.

    Abstract: Classical pharmacology is normally concerned with defined molecular structures that can bind to specific proteins and either inhibit or enhance the protein function to achieve some biological response with therapeutic benefit. In photodynamic therapy (PDT) context, we rarely rely on such target specificity to achieve therapeutic success. Although some recent photosensitizers have been functionalized with target-specific molecules, such as antibodies, to recognize specific cells and enhance therapy specificity, ROS produced inside the cell will damage all susceptible molecules within the diffusion radius. According to the previous chapter, both hydroxyl radicals and singlet oxygen are highly reactive toward most of the abundant biological molecules contained in cells. In this chapter we discuss how such capacity of PDT to provoke multiple sites of molecular damages in the cellular context is associated with the phototoxicity produced. Also, we discuss how cellular antioxidant and xenobiotic defenses can influence on cellular tolerance against photodynamic inactivation.


  • IPEN-DOC 20928

    SELLERA, FABIO P.; BARBOSA, BRUNA S.; GARGANO, RONALDO G.; SABINO, CAETANO P. ; RIBEIRO, MARTHA S. ; AZEVEDO, MILTON R.; BENESI, FERNANDO J.; POGLIANI, FABIO C.. Cutaneous streptococcal abscess treated by photodynamic therapy. African Journal of Traditional Complementary and Alternative Medicines, v. 12, n. 2, p. 65-67, 2015.

    Palavras-Chave: therapy; skin diseases; sheep; streptococcus; infectivity; photosensitivity


  • IPEN-DOC 21964

    SABINO, CAETANO P. ; RIBEIRO, MARTHA S. . Desenvolvimento de um modelo in vivo para o estudo do efeito fotodinâmico com infecção fúngica. In: PROGRAMA INSTITUCIONAL DE BOLSAS DE INICIAÇÃO CIENTÍFICA, 20.; PROGRAMA DE BOLSAS E INICIAÇÃO CIENTÍFICA CNEN, 11.; PROGRAMA INSTITUCIONAL DE BOLSAS DE INICIAÇÃO DESENVOLVIMENTO TECNOLÓGICO E INOVAÇÃO, 4., 22-23 de outubro, 2014, São Paulo, SP. Resumo expandido... 2014. p. 100-101.

    Palavras-Chave: in vivo; therapy; lasers; fungal diseases; candida; animals; mice; female genitals; antimicrobial agents


  • IPEN-DOC 26934

    CABRAL, FERNANDA V. ; SILVA, CAMILA R. ; SAUTER, ISMAEL P.; SABINO, CAETANO P. ; YOSHIMURA, TANIA M. ; CORTEZ, MAURO; RIBEIRO, MARTHA S. . Efeito da terapia fotodinâmica no tratamento de leishmaniose cutânea em um modelo murino. Anais da SBBN, v. 3, p. 67-77, 2016.

    Abstract: Leishmanioses são doenças parasitárias desenvolvidas por protozoários do gênero Leishmania. A forma cutânea abrange lesões destrutivas e ulceradas com diversas limitações no tratamento. Terapias alternativas são fundamentais devido à grande relevância da doença e elevada incidência. Nesse contexto, a terapia fotodinâmica (PDT) tem sido introduzida devido ao baixo custo, baixa toxicidade, praticidade e sem relatos de resistência na literatura. Neste trabalho, dezesseis camundongos BALB/c foram infectados com 1.106 parasitos de Leishmania(L) amazonensisno membro posterior esquerdo e acompanhados por 4 semanas até o surgimento da lesão. Após esse período, os animais foram submetidos à PDT usando um diodo emissor de luz (λ=660 ±22 nm) e azul de metileno (100 μM), com fluências de 50, 100 e 150J/cm² e acompanhados durante 3 semanas. Animais controle não receberam tratamento. O tamanho da lesão e escala de dor foram mensurados utilizando um paquímetro e filamentos von Frey, respectivamente. A quantificação da carga parasitária foi realizada através do método de diluição limitante. Os resultados demonstraram que, nas semanas 2 e 3 após tratamento, ocorreu diminuição da lesão e alívio de dor nos animais que receberam 150J/cm², sugerindo que a PDT promoveu melhora clínica através da modulação do processo inflamatório.

    Palavras-Chave: photosensitivity; methylene blue; parasites; parasitic diseases; biological models; epithelium; light sources; light emitting diodes; therapy; skin diseases


  • IPEN-DOC 24497

    SELLERA, FABIO P.; NASCIMENTO, CRISTIANE L.; POGLIANI, FABIO C.; SABINO, CAETANO P. ; RIBEIRO, MARTHA S. . Future perspectives. In: SELLERA, FABIO P. (Ed.); NASCIMENTO, CRISTIANE L. (Ed.); RIBEIRO, MARTHA S. (Ed.). Photodynamic therapy in veterinary medicine: from basics to clinical practice. Gewerbestrasse, Switzerland: Springer, 2016. p. 209-222, DOI: 10.1007/978-3-319-45007-0_14

    Observação: Livro na íntegra disponível. Consulte a biblioteca do IPEN.

    Abstract: Nowadays, it is clear that the activity of different photosensitizers (PSs) has a strong potential for moving photodynamic therapy (PDT) to clinical practice. Present technologies as dedicated light sources, new PSs, and nanotechnology are emerging strategies to promote PDT as a reliable, cost-effective, and safe approach to veterinary medicine. This chapter addresses an overview of emerging clinical applications and recent technologies to encourage veterinarians toward PDT.


  • IPEN-DOC 23521

    SUZUKI, LUIS C. ; KATO, ILKA T.; PRATES, RENATO A.; SABINO, CAETANO P. ; YOSHIMURA, TANIA M. ; SILVA, TAMIRES O.; RIBEIRO, MARTHA S. . Glucose modulates antimicrobial photodynamic inactivation ofCandida albicans in biofilms. Photodiagnosis and Photodynamic Therapy, v. 17, p. 173-179, 2017. DOI: 10.1016/j.pdpdt.2016.12.003

    Abstract: Candida albicans biofilm is a main cause of infections associated with medical devices such as catheters,contact lens and artificial joint prosthesis. The current treatment comprises antifungal chemotherapy thatpresents low success rates. Photodynamic inactivation (PDI) involves the combination of a photosensitiz-ing compound (PS) and light to generate oxidative stress that has demonstrated effective antimicrobialactivity against a broad-spectrum of pathogens, including C. albicans. This fungus senses glucose induc-ing an upregulation of membrane transporters that can facilitate PS uptake into the cell. The aim ofthis study was to evaluate the effects of glucose on methylene blue (MB) uptake and its influence onPDI efficiency when combined to a red LED with central wavelength at = 660 nm. C. albicans biofilmswere grown on hydrogel disks. Prior to PDI assays, MB uptake tests were performed with and withoutglucose-sensitization. In this system, the optimum PS administration was determined as 500 M of MBin contact with the biofilm during 30 min before irradiation. Irradiation was performed during 3, 6, 9, 12,15 and 18 min with irradiance of 127.3 mW/cm2. Our results showed that glucose was able to increaseMB uptake in C. albicans cells. In addition, PDI without glucose showed a higher viability reduction until6 min; after 9 min, glucose group demonstrated a significant decrease in cell viability when compared toglucose-free group. Taken together, our data suggest that glucose is capable to enhance MB uptake andmodulate photodynamic inactivation of C. albicans biofilm.


  • IPEN-DOC 24448

    SELLERA, FABIO P.; SABINO, CAETANO P. ; HAMBLIN, MICHAEL R.. History of PDT. In: SELLERA, FABIO P. (Ed.); NASCIMENTO, CRISTIANE L. (Ed.); RIBEIRO, MARTHA S. (Ed.). Photodynamic therapy in veterinary medicine: from basics to clinical practice. Gewerbestrasse, Switzerland: Springer, 2016. p. 1-10, DOI: 10.1007/978-3-319-45007-0_1

    Observação: Livro na íntegra disponível. Consulte a biblioteca do IPEN.

    Abstract: This chapter presents the brightest historical milestones behind the development of photodynamic therapy (PDT). We initially present how photodynamic reactions were first observed by scientists from three different countries in the beginning of the twentieth century. Oskar Raab, from Germany, observed by accident that protozoan cells stained with fluorescent dyes were killed upon illumination, while Prime, in France, reported that human subjects who ingested also fluorescent dyes for an experimental treatment of neurological diseases developed severe erythema after short exposure to sunlight. Niels Finsen, from Denmark, was awarded with the third Nobel Prize of Medicine in the history for the development of light-based treatments for skin infections. Following, we describe how PDT slowly evolved until the 1960–1970s when new generations of less toxic photosensitizers were developed for diagnosis and treatment of solid tumors. Only then PDT really became a hot scientific area that began to attract many researchers to the field. We also describe the first huge medical and economic impact that PDT as the first effective treatment for age-related macular degeneration, the leading cause of adult blindness in the world. Finally, we go through the main discoveries in veterinary medicine over the past years for the treatment of localized tumors and infections in diverse animal species.


  • IPEN-DOC 22388

    SELLERA, FABIO P.; SABINO, CAETANO P. ; RIBEIRO, MARTHA S. ; GARGANO, RONALDO G.; BENITES, NILSON R.; MELVILLE, PRISCILLA A.; POGLIANI, FABIO C.. In vitro photoinactivation of bovine mastitis related pathogens. Photodiagnosis and Photodynamic Therapy, v. 13, p. 276-281, 2016.

    Palavras-Chave: in vitro; cows; therapy; veterinary medicine; diagnosis; antibiotics; pathogens; cattle; methylene blue


  • IPEN-DOC 23888

    SOUSA, MARCELO V.P.; PRATES, RENATO ; KATO, ILKA T. ; SABINO, CAETANO P. ; YOSHIMURA, TANIA M. ; SUZUKI, LUIS C. ; MAGALHAES, ANA C.; YOSHIMURA, ELISABETH M.; RIBEIRO, MARTHA S. . Inhomogeneity in optical properties of rat brain: a study for LLLT dosimetry. In: HAMBLIN, MICHAEL R. (Ed.); ARANY, PRAVEEN R. (Ed.); CARROLL, JAMES D. (Ed.) MECHANISMS FOR LOW-LIGHT THERAPY, 8th, February 02, 2013, San Francisco, CA, USA. Proceedings... Bellingham, WA, USA: International Society for Optics and Photonics, 2013. p. 856905-1 - 856905-6. (SPIE Proceedings Series, 8569). DOI: 10.1117/12.2002836

    Abstract: Over the last few years, low-level light therapy (LLLT) has shown an incredible suitability for a wide range of applications for central nervous system (CNS) related diseases. In this therapeutic modality light dosimetry is extremely critical so the study of light propagation through the CNS organs is of great importance. To better understand how light intensity is delivered to the most relevant neural sites we evaluated optical transmission through slices of rat brain point by point. We experimented red (λ = 660 nm) and near infrared (λ = 808 nm) diode laser light analyzing the light penetration and distribution in the whole brain. A fresh Wistar rat (Rattus novergicus) brain was cut in sagittal slices and illuminated with a broad light beam. A high-resolution digital camera was employed to acquire data of transmitted light. Spatial profiles of the light transmitted through the sample were obtained from the images. Peaks and valleys in the profiles show sites where light was less or more attenuated. The peak intensities provide information about total attenuation and the peak widths are correlated to the scattering coefficient at that individual portion of the sample. The outcomes of this study provide remarkable information for LLLT dose-dependent studies involving CNS and highlight the importance of LLLT dosimetry in CNS organs for large range of applications in animal and human diseases.


  • IPEN-DOC 25029

    SABINO, CAETANO P. ; BAPTISTA, MAURICIO da S.; RIBEIRO, MARTHA S. ; LINCOPAN, NILTON. Methylene blue uptake and intermolecular interactions in microbial cells through Fluorescence Lifetime Imaging Microscopy (FLIM). In: INTERNATIONAL PHOTODYNAMIC ASSOCIATION WORLD CONGRESS, 16th, June 08-13, 2017, Coimbra, Portugal. Abstract... 2017. p. 82-82.

    Abstract: Antimicrobial photodynamic therapy (APDT) is a promising tool to counterattack the emerging treat of drug-resistant pathogens. The technique combines low-intensity monochromatic light with a photosensitizer compound to produce reactive oxygen species (ROS) that can damage virtually any type of biomolecules and lead to rapid ce\l death. Since some ROS present diffusion-limited reactivity, most cell damage is co-localized with photosensitizer accumulation site. Hence, imaging photosensitizer accumulation and fluorescence lifetime in the nanoscale can bring a great levei of information to further understand the ultrastructural cellular damage caused by APDT. In this study, we used a FLIM system capable of single-molecule detection to observe the accumulation and interaction sites of methylene blue (MB), a very broadly-used photosensitizer, in yeast, and Gram-positive and Gram-negative bacterial cells, Our data shows fluorescence lifetime contrast, with nanometric resolution, among different cellular structures such as cell wall, membrane and DNA. The images evidentiate differential MB accumulation in microbial cells and the existence of two different populations of MB molecular species: those interacting mostly with the solvent (short-lived, - 0.8 ns) and those interacting with biomolecules (Iong-lived, -2 ns), The short-lived fluorescence predominates in the mucoid capsule of Gram-negative bacteria and cell-wall ofyeast and Gram-positive bacteria while longlived MB fluorescence shows preferential accumulation in DNA-rich sites 1 • It is marked in yeast nucleus and exclusively inside bacterial cells. In fact, literature supports that MB intercalation in nucleic acids stabilizes its excited-states leading to increased "fluorescence "lifetime and efficiency of singlet-oxygen production2 . Our data brings evidence that this sOli of phenomena can be observed by FLIM in the nanoscale and this should bring new insights to the photophysical, photochemical and biological mechanisms of photodynamic therapy.


  • IPEN-DOC 21874

    SABINO, CAETANO P. ; RIBEIRO, MARTHA S. . Modelo de estudo in vitro de infecções por biofilme de Candida albicans e sua inativação por terapia fotodinâmica. In: PROGRAMA INSTITUCIONAL DE BOLSAS DE INICIAÇÃO CIENTÍFICA, 19.; PROGRAMA DE BOLSAS E INICIAÇÃO CIENTÍFICA CNEN, 10.; PROGRAMA INSTITUCIONAL DE BOLSAS DE INICIAÇÃO DESENVOLVIMENTO TECNOLÓGICO E INOVAÇÃO, 3., 23-24 de outubro, 2013, São Paulo, SP. Resumo expandido... 2013.

    Palavras-Chave: in vitro; infectious diseases; candida; inactivation; therapy; lasers; films; teeth


  • IPEN-DOC 24457

    SABINO, CAETANO P. ; HAMBLIN, MICHAEL R.. Molecular damage. In: SELLERA, FABIO P. (Ed.); NASCIMENTO, CRISTIANE L. (Ed.); RIBEIRO, MARTHA S. (Ed.). Photodynamic therapy in veterinary medicine: from basics to clinical practice. Gewerbestrasse, Switzerland: Springer, 2016. p. 45-56, DOI: 10.1007/978-3-319-45007-0_4

    Observação: Livro na íntegra disponível. Consulte a biblioteca do IPEN.

    Abstract: Photodynamic therapy (PDT) rapidly produces large amounts of reactive oxygen species (ROS) to induce death of photosensitized cells. As previously described in Chap. 2, excited photosensitizer (PS) molecules can either donate electrons (type 1) or energy (type 2) to ground-state oxygen to produce superoxide radicals (O2•−) or singlet oxygen (1O2). Each type of ROS has characteristic chemical reactivity and reacts with different types of chemical bonds present in biomolecules and, consequently, will lead to different types of cell damage. Once again, what determines the mechanism of cell death directly depends on both: the PS localization site within the cell and total extent of oxidative stress produced during therapy (i.e., light dosimetry and efficiency of ROS generation). To elucidate the mechanisms of photooxidative damage and the consequent biological effects, this chapter will cover the most relevant chemical reactions related to oxidative damage caused by 1O2 and free radicals.


  • IPEN-DOC 21613

    BAPTISTA, ALESSANDRA ; NUNEZ, SILVIA C. ; SABINO, CAETANO P. ; MIYAKAWA, WALTER ; RIBEIRO, MARTHA S. . Morphological evaluation of Candida albicans after phododynamic therapy. In: INTERNATIONAL PHOTODYNAMIC ASSOCIATION WORLD CONGRESS, May 23-26, 2015, Rio de Janeiro, RJ. Abstract... 2015. p. 355.

    Palavras-Chave: morphological changes; candida; therapy; antimicrobial agents; scanning electron microscopy; atomic force microscopy; photosensitivity


  • IPEN-DOC 21600

    BAPTISTA, ALESSANDRA ; NUNEZ, SILVIA C. ; SABINO, CAETANO P. ; MIYAKAWA, WALTER ; RIBEIRO, MARTHA S. . Morphological evaluation of Candida albicans after phododynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 12, p. 335, 2015.

    Palavras-Chave: morphological changes; candida; therapy; antimicrobial agents; scanning electron microscopy; atomic force microscopy; photosensitivity


  • IPEN-DOC 24463

    RIBEIRO, MARTHA S. ; SABINO, CAETANO P. . Multimodality dosimetry. In: SELLERA, FABIO P. (Ed.); NASCIMENTO, CRISTIANE L. (Ed.); RIBEIRO, MARTHA S. (Ed.). Photodynamic therapy in veterinary medicine: from basics to clinical practice. Gewerbestrasse, Switzerland: Springer, 2016. p. 93-109, DOI: 10.1007/978-3-319-45007-0_7

    Observação: Livro na íntegra disponível. Consulte a biblioteca do IPEN.

    Abstract: PDT requires a multimodality approach for dosimetry because it works based on three essential components: light, photosensitizer, and molecular oxygen. Since these components are found in variable amounts inside target cells, PDT dosimetry is rather intricate. This chapter intends to address, with little mathematical complexity, the physical and chemical quantities that are most relevant for light and photosensitizer dosimetry as well as to present basic aspects of oxygen supply to achieve successful PDT interventions.


  • IPEN-DOC 25800

    DIMMER, JESICA; CABRAL, FERNANDA V. ; SABINO, CAETANO P. ; SILVA, CAMILA R. ; NUNEZ-MONTOYA, SUSANA C.; CABRERA, JOSE L.; RIBEIRO, MARTHA S. . Natural anthraquinones as novel photosentizers for antiparasitic photodynamic inactivation. Phytomedicine, v. 61, n. 152894, p. 1-7, 2019. DOI: 10.1016/j.phymed.2019.152894

    Abstract: Background: Cutaneous leishmaniasis (CL) is a vector-borne disease caused by obligate protist parasites from the genus Leishmania. The potential toxicity as well as the increased resistance of standard treatments has encouraged the development of new therapeutical strategies. Photodynamic inactivation (PDI) combines the use of a photosensitizer and light to generate reactive oxygen species and kill cells, including microorganisms. Vegetal kingdom constitutes an important source of bioactive compounds that deserve to be investigated in the search of naturally occurring drugs with leishmanicidal activity. Purpose: The purpose of this study was to test the antiparasitic activity of PDI (ApPDI) of five natural anthraquinones (AQs) obtained from Heterophyllaea lycioides (Rusby) Sandwith (Rubiacae). To support our results, effect of AQ mediated-PDI on parasite´s morphology and AQ uptake were studied. Cytotoxicity on fibroblasts was also evaluated. Study design/Methods: Two monomers, soranjidiol (Sor) and 5-chlorosoranjidiol (5-ClSor) plus three bi-anthraquinones (bi-AQs), bisoranjidiol (Bisor), 7-chlorobisoranjidiol (7-ClBisor) and Lycionine (Lyc) were selected for this study. Recombinant L. amazonensis promastigote strain expressing luciferase was subjected to AQs and LED treatment. Following irradiation with variable light parameters, cell viability was quantified by bioluminescence. Alteration on parasite's morphology was analyzed by scanning electron microscopy (SEM). In addition, we verified the AQ uptake in Leishmania cells by fluorescence and their toxicity on fibroblasts by using MTT assay. Results: Bisor, Sor and 5-ClSor exhibited photodynamic effect on L. amazonensis. SEM showed that promastigotes treated with Bisor-mediated PDI exhibited a significant alteration in shape and size. Sor and 5-ClSor presented higher uptake levels than bi-AQs (Bisor, Lyc and 7-ClBisor). Finally, Sor and Bisor presented the lowest toxic activity against fibroblasts. Conclusion: Taking together, our results indicate that Sor presents the highest specificity towards Leishmania cells with no toxicity on fibroblasts.

    Palavras-Chave: parasitic diseases; anthraquinones; antimitotic drugs; therapy; photosensitivity; inactivation; monomers


  • IPEN-DOC 24496

    SELLERA, FABIO P.; POGLIANI, FABIO C.; SABINO, CAETANO P. . Other practices in PDT. In: SELLERA, FABIO P. (Ed.); NASCIMENTO, CRISTIANE L. (Ed.); RIBEIRO, MARTHA S. (Ed.). Photodynamic therapy in veterinary medicine: from basics to clinical practice. Gewerbestrasse, Switzerland: Springer, 2016. p. 197-207, DOI: 10.1007/978-3-319-45007-0_13

    Observação: Livro na íntegra disponível. Consulte a biblioteca do IPEN.

    Abstract: In addition to clinical PDT applications regarding antimicrobial and antineoplastic activity, photodynamic reactions have also been used in several other practices such as for fish tank decontamination, water treatment, antiangiogenic therapy for age-related macular degeneration, decontamination of surfaces, and even inactivation of pathogens for blood transfusion. Nowadays, not all potentials of photodynamic reactions are commercially available yet, but they definitely deserve to be highlighted in this chapter as alternative applications of photodynamic reactions in veterinary medicine.


  • IPEN-DOC 24332

    BAPTISTA, ALESSANDRA ; SABINO, CAETANO P. ; NUNEZ, SILVIA C.; MIYAKAWA, WALTER; MARTIN, AIRTON A.; RIBEIRO, MARTHA S. . Photodynamic damage predominates on different targets depending on cell growth phase of Candida albicans. Journal of Photochemistry & Photobiology, B: Biology, v. 177, p. 76-84, 2017. DOI: 10.1016/j.jphotobiol.2017.10.013

    Abstract: Photodynamic inactivation (PDI) has been reported to be effective to eradicate a wide variety of pathogens, including antimicrobial-resistant microorganisms. The aim of this study was to identify the potential molecular targets of PDI depending on growth phase of Candida albicans. Fungal cells in lag (6 h) and stationary (48 h) phases were submitted to PDI mediated by methylene blue (MB) combined with a (662 +/- 21) nm-LED, at 360 mW of optical power. Pre-irradiation time was 10 min and exposure times were 12 min, 15 min and 18 min delivering radiant exposures of 129.6 J/cm(2), 162 J/cm(2) and 194.4 J/cm(2), respectively, on a 24-well plate of about 2 cm(2) at an irradiance of 180 mW/cm(2). Scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force spectroscopy (AFS) and Fourier transform infrared spectroscopy (FT-IR) were employed to evaluate the photodynamic effect in young and old fungal cells following 15 min of irradiation. Morphological analysis revealed wrinkled and shrunk fungal cell membrane for both growth phases while extracellular polymeric substance (EPS) removal was only observed for old fungal cells. Damaged intracellular structures were more pronounced in young fungal cells. The surface nanostiffness of young fungal cells decreased after PDI but increased for old fungal cells. Cellular adhesion force was reduced for both growth phases. Fungal cells in lag phase predominantly showed degradation of nucleic acids and proteins, while fungal cells in stationary phase showed more pronounced degradation of polysaccharides and lipids. Taken together, our results indicate different molecular targets for fungal cells in lag and stationary growth phase following PDI.


  • IPEN-DOC 20713

    SELLERA, FABIO P.; SABINO, CAETANO P. ; RIBEIRO, MARTHA S. ; FERNANDES, LORIE T.; POGLIANI, FABIO C.; TEIXEIRA, CARLOS R.; DUTRA, GUSTAVO H.P.; NASCIMENTO, CRISTIANE L.. Photodynamic therapy for pododermatitis in penguins. Zoo Biology, v. 33, n. 4, p. 353-356, 2014.

    Palavras-Chave: birds; dermatitis; antimicrobial agents; methylene blue; healing; wounds; photosensitivity; therapy


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Na página do pesquisador, é possível verificar, as variações do nome, a relação de todos os trabalhos com texto completo bem como um quadro resumo numérico; há links para o Currículo Lattes e o Google Acadêmico ( quando esse for informado).



O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.

A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

1. Portaria IPEN-CNEN/SP nº 387, que estabeleceu os princípios que nortearam a criação do RDI, clique aqui.

2. A experiência do Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) na criação de um Repositório Digital Institucional – RDI, clique aqui.