Navegação por Revista "Lasers in Medical Science"

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  • IPEN-DOC 12314

    BOTTA, SERGIO B.; ANA, PATRICIA A. da; ZEZELL, DENISE M. ; POWERS, JOHN M.; MATOS, ADRIANA B.. Adhesion after erbium, chromium: yttrium-scandium-gallium-garnet laser aplication at three different irradiation conditions. Lasers in Medical Science, v. 24, n. 1, p. 67-73, 2009.

    Palavras-Chave: erbium; chromium; yttrium; scandium; gallium; garnets; laser radiation; teeth; caries; tissues; cooling; water; bonding


  • IPEN-DOC 13632

    WETTER, NIKLAUS U. ; BRANCO, ELOISA P.; DEANA, ALESSANDRO M.; PELINO, JOSÉ E.P.. Color differences of canines and incisors in a comparative long-therm clinical trial of three bleaching systems. Lasers in Medical Science, v. 24, p. 941-947, 2009.

    Palavras-Chave: teeth; coloration; bleaching; spectrophotometers; lasers; light emitting diodes; biological materials


  • IPEN-DOC 15926

    THEODORO, LETICIA H.; ZEZELL, DENISE M. ; GARCIA, VALDIR G.; HAYPEK, PATRICIA; NAGATA, MARIA J.H.; ALMEIDA, JULIANO M. de; EDUARDO, CARLOS de P.. Comparative analysis of root surface smear layer removal by different etching modalities or erbium: yttrium-aluminium-garnet laser irradiation. A scanning electron microscopy study. Lasers in Medical Science, v. 25, n. 4, p. 485-491, 2010.

    Palavras-Chave: biological radiation effects; citric acid; comparative evaluations; dentin; dentistry; edta; erbium; laser radiation; lasers; scanning electron microscopy; teeth


  • IPEN-DOC 19112

    MAROTTI, JULIANA; TORTAMANO, PEDRO; CAI, SILVANA; FRANCO, JOÃO E.M.; CAMPOS, TOMIE T. de. Decontamination of dental implant surfaces by means of photodynamic therapy. Lasers in Medical Science, v. 28, n. 3, p. 303-309, 2013.

    Palavras-Chave: teeth; implants; titanium; surface area; surface treatments; bacteria; decontamination; antimicrobial agents; methylene blue; lasers; photosensitivity; therapy


  • IPEN-DOC 16447

    SPERANDIO, FELIPE F.; MENEGUZZO, DAIANE T.; FERREIRA, LEILA S.; ANA, PATRICIA A. da; AZEVEDO, LUCIANE H.; SOUSA, SUZANA C.O.M. de. Different air-water spray regulations affect the healing of Er,Cr:YSGG laser incisions. Lasers in Medical Science, v. 26, n. 2, p. 257-265, 2011.

    Palavras-Chave: laser radiation; erbium; chromium; wounds; healing; sprays; histological techniques; immunity


  • IPEN-DOC 18105

    CUNHA, C.B.; EDUARDO, F.P.; ZEZELL, D.M. ; BEZINETTI, L.M.; SHITARA, P.P.L.; CORREA, L.. Effect of irradiation with red and infrared laser in the treatment of oral mucositis : A pilot study with patients undergoing chemotherapy with 5-FU. Lasers in Medical Science, v. 27, n. 6, p. 1233-1240, 2012.

    Palavras-Chave: radiation effects; infrared spectra; oral cavity; mucous membranes; chemotherapy; radiotherapy


  • IPEN-DOC 14086

    SAWAZAKI, IRIS; MARTINS, VINICIUS R.G.; RIBEIRO, MARTHA S. ; MARQUES, MARCIA M.. Effect of low-intensity therapy on mast cell degranulation in human oral mucosa. Lasers in Medical Science, v. 24, n. 1, p. 113-116, 2009.

    Palavras-Chave: laser radiation; low dose irradiation; mast cells; radiolysis; mucous membranes; oral cavity


  • IPEN-DOC 24320

    SUZUKI, SELLY S. ; GARCEZ, AGUINALDO S.; REESE, PATRICIA O.; SUZUKI, HIDEO; RIBEIRO, MARTHA S. ; MOON, WON. Effects of corticopuncture (CP) and low-level laser therapy (LLLT) on the rate of tooth movement and root resorption in rats using micro-CT evaluation. Lasers in Medical Science, v. 33, n. 4, p. 811-821, 2018. DOI: 10.1007/s10103-017-2421-5

    Abstract: The aim of this study was to compare the rate of tooth displacement, quantity of root resorption, and alveolar bone changes in five groups: corticopuncture (CP), low-level laser therapy (LLLT), CP combined with LLLT (CP + LLLT), control (C), and negative control (NC). A total of 60 half-maxilla from 30 male Wistar rats (10 weeks old) were divided randomly into five groups: three (CP, LLLT, and CP + LLLT) test groups with different stimulation for accelerated-tooth-movement (ATM), one control (C) group, and one negative control (NC) group with no tooth movement. Nickel-titanium coil springs with 50 g of force were tied from the upper left and right first molars to micro-implants placed behind the maxillary incisors. For the CP and CP + LLLT groups, two perforations in the palate and one mesially to the molars were performed. For the LLLT and CP + LLLT groups, GaAlAs diode laser was applied every other day for 14 days (810 nm, 100 mW, 15 s). The tooth displacements were measured directly from the rat’s mouth and indirectly from microcomputer (micro-CT) tomographic images. Bone responses at the tension and compression sites and root resorption were analyzed from micro-CT images. The resulting alveolar bone responses were evaluated by measuring bone mineral density (BMD), bone volume fraction (BV/TV), and trabecular thickness (TbTh). Root resorption crater volumes were measured on both compression and tension sides of mesial and distal buccal roots. The tooth displacement in the CP + LLLT group was the greatest when measured clinically, followed by the CP, LLLT, and control groups (C and NC), respectively (p <0.05). The tooth movements measured from micro-CT images showed statistically higher displacement in the CP and CP + LLLT groups compared to the LLLT and control groups. The BMD, BV/TV, and TbTh values were lower at the compression side and higher at the tension side for all three test groups compared to the control group. The root resorption crater volume of the distal buccal root was higher in the control group, followed by CP, LLLT, and CP + LLLT, mostly at the compression site. Combining corticopuncture and low-level laser therapy (CP + LLLT) produced more tooth displacement and less root resorption at the compression side. The combined technique also promoted higher alveolar bone formation at the tension side.


  • IPEN-DOC 26888

    CONTATORI, C.G. de S. ; SILVA, C.R. ; YOSHIMURA, T.M. ; RIBEIRO, M.S. . Effects of low-level laser irradiation on VEGF expression of melanoma cell lines. Lasers in Medical Science, v. 35, n. 1, p. 269-269, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Impact of low-level laser irradiation on tumor cell lines remains controversial. Vascular endothelial growth factor (VEGF) is a key molecule to form new blood vessels, which contribute for cancer development and growth. The aim of this study was to evaluate the effects of different light fluences on human melanoma SKMEL 37 cells and murine melanoma B16F10 cells using a near infrared laser (λ= 780 nm) with output power of 40 mW delivering energies of 1.2, 3.6 and 6 J (fluences of 30, 90 and 150 J/cm2, respectively). The cell lines were irradiated 24 h after they were seeded in a 96-well plate at a density of 5x103 cells per well, in triplicate at three different days. Following irradiation, both cell line supernatants were stored in Eppendorf tubes at - 20°C until VEGF-A expression measurement. Specific ELISA kits were used according to cell line (murine or human). Samples and standard solutions were added in a 96-well plate antibody-coated and incuba ted over night at 4°C. Reagent dilution and set time followed fabricant instructions. The stop solution was added and the absorbance was read in a microplate reader at 450 nm. Results showed a non-statistically significant difference among treated and control groups for both cell lines. These findings indicate that irradiation with near infrared laser does not influence VEGF expression on melanoma cell lines regardless the fluence used and should be tested to prevent cancer growth in preclinical assays.

    Palavras-Chave: laser radiation; low dose irradiation; doses; irradiation; tumor cells; melanomas; enzyme immunoassay


  • IPEN-DOC 26889

    SILVA, C.R. ; RIBEIRO, M.S. . Effects of photobiomodulation on breast tumor- bearing mice before radiotherapy. Lasers in Medical Science, v. 35, n. 1, p. 269-269, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Photobiomodulation (PBM) has been studied to modify the cellular response to ionizing radiation. However, its combination with radiotherapy (RT) has not been reported in cancer treatment. The aim of this study was to evaluate the effects of PBM applied before RT on breast tumor-bearing mice. Female BALB/c mice were inoculated with breast 4T1 cells into mammary fat pad and divided into 4 groups (n =5 per group): control (with no treatment), only RT, and PBM combined to RT in two different protocols. RT was locally applied using a 60Co source with dose of 60 Gy in fractions of 15 Gy. For PBM, a red laser (660 nm, 500 mW/cm2) was used in two regimes: single exposure 24 h before RT (fluence of 150 J/cm2) and immediately before each RT session (fluence of 37.5 J/cm2 per session). After treatment, tumor volume, platelets, white and red blood cell levels were evaluated during 14 days. Our results showed no statistically significant differen ces in t umor volume, platelet and red blood cell levels comparing control, RT and PBM+RT groups. However, PBM was able to sustain normal white blood cell levels compared to RT and control groups. In addition, mice that received PBM concomitant with RT presented a longer survival. In fact, for this group only 12.5 % of the animals died during experimental period. These findings indicate that PBM could be combined to RT to provide therapeutic anti-cancer benefits.

    Palavras-Chave: ionizing radiations; therapy; neoplasms; tumor cells; mice; mammary glands; radiotherapy


  • IPEN-DOC 25062

    BEZERRA, S.J.C.; TREVISAN, L.R.; VIANA, I.E.L.; LOPES, R.M.; PEREIRA, D.L. ; ARANHA, A.C.C.; SCARAMUCCI, TAIS. Er,Cr:YSGG laser associated with acidulated phosphate fluoride gel (1.23% F) for prevention and control of dentin erosion progression. Lasers in Medical Science, v. 34, n. 3, p. 449-455, 2019. DOI: 10.1007/s10103-018-2609-3

    Abstract: To evaluate the effect of Er,Cr:YSGG laser, associated with fluoride application, on the prevention/control of dentin erosion. Dentin slabs were embedded in acrylic resin, flattened, and polished. Half of the specimens were previously eroded (10 min immersion in 1% citric acid solution) and half were kept sound. The specimens (n = 10 each substrate) were randomly allocated into the experimental groups, according to the following treatments: control (no treatment); APF gel (1.23% F, 1 min); Er,Cr:YSGG laser irradiation (P1: 0.25W, 20 Hz, 2.8 J/cm2, tip S75, beam diameter of 750 μm, 1mmaway fromthe surface); Er,Cr:YSGG laser irradiation (P2: 0.50W, 20 Hz, 5.7 J/cm2, tip S75, beam diameter of 750 μm, 1 mm away from the surface); APF gel + Er,Cr:YSGG laser P1 and; APF gel + Er,Cr:YSGG laser P2. Afterwards, the specimens underwent an erosion-remineralization cycling, consisting of a 5-min immersion into 0.3% citric acid, followed by 60-min exposure to artificial saliva. This procedure was repeated 4×/day, for 5 days. Surface loss (SL, in μm) was determined by optical profilometry. Specimens from each group were analyzed by environmental scanning electron microscopy (n = 3). Data were statistically analyzed (α = 0.05). For the eroded specimens, APF gel presented the lowest SL, being different from the control. For the sound specimens, none of the groups differed from the control, except for Er,Cr:YSGG laser P2, which presented the highest SL.When substrates were compared, only the eroded specimens of the control and APF + Er,Cr:YSGG laser P1 Groups showed higher SL. Selective structure removal was observed for the laser-treated groups. None of the Er,Cr:YSGG laser parameters were effective in the prevention/control dentin erosion. The laser was also unable to enhance the protection of fluoride against dentin erosion.

    Palavras-Chave: citric acid; dentin; fluorides; gels; laser radiation; lasers; resins; saliva; scanning electron microscopy; sound waves; teeth


  • IPEN-DOC 15937

    ROMANO, ANA C.C.C.; ARANHA, ANA C.C.; SILVEIRA, BRUNO L. da; BALDOCHI, SONIA L. ; EDUARDO, CARLOS de P.. Evaluation of carbon dioxide laser irradiation associated with calcium hydroxide in the treatment of dentinal hypersensitivity. A preliminary study. Lasers in Medical Science, v. 26, p. 35-42, 2011.

    Palavras-Chave: dentin; morphological changes; laser radiation; calcium hydroxides; sensitivity


  • IPEN-DOC 12814

    BEVILACQUA, FLAVIA M.; ZEZELL, DENISE M. ; MAGNANI, ROMEU; ANA, PATRICIA A. da; EDUARDO, CARLOS de P.. Fluoride uptake and acid resistance of enamel irradiated with Er:YAG laser. Lasers in Medical Science, v. 23, p. 141-147, 2008.

    Palavras-Chave: teeth; caries; fluorides; apatites; enamels; demineralization


  • IPEN-DOC 26890

    NUNEZ, SILVIA C.; SILVA, IVES C. da; NASCIMENTO, JONATHAN S.; RIBEIRO, MARTHA S. . Methodological study of photobiomodulation anti-edematous effects on carrageenan-induced inflammation on zebrafish. Lasers in Medical Science, v. 35, n. 1, p. 288-289, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: The anti-inflammatory action of Photobiomodulation (PBM) is one of its most reproducible effects in vivo. New models to study PBMeffects and to investigate light dosimetry may bring different perspectives on the field. Based on the importance of establishing new experimental models and the advantages of using zebrafish for screening new drugs with potential anti-inflammatory effects the present investigation evaluated the effect of PBM on carragenan-induced abdominal edema in zebrafish. Thirty Danio rerio (± 1 g) zebrafish were randomly divided into five aquariums with 0.25L of water to establish three treatments: T1 - Naive non-treated; T2 – Saline 0.9%-injected; T3 - carrageenan-injected (CG) 3.5% in the coelomic cavity. T4 –injected (CG) 3.5% and treated with a diode laser λ=808nm, 50mWand 0.5J T5 –same parameters with 1J and T6 -1.5J. Abdominal volumes were measured by a caliper and the area was calculated 4 hours post-indu ction. Four hours after edema induction the results revealed an increase in the abdominal area of fish injected with CG 3.5% compared to negative control. The animals injected with CG and treated with PBM exhibited a reduction in abdominal edema in a dose-dependent manner. The 0.5J energy did not present a significant reduction compare do control (p=0.18), the 1J and 1.5J groups presented a significant difference (p=0.027 and p=0.005 respectively). The 1.5J group was not different from negative control (T2) p=0.37. The present study identified that zebrafish may represent a good model to investigate PBM effects and the response was dose dependent.

    Palavras-Chave: inflammation; fishes; antipyretics; dosimetry; biological effects


  • IPEN-DOC 12315

    ANDRADE, ANA K.P. de; FEIST, ILIRIA S.; PANNUTI, CLAUDIO M.; CAI, SILVANA; ZEZELL, DENISE M. ; MICHELI, GIORGIO de. Nd:YAG laser clinical assisted in class II furcation treatment. Lasers in Medical Science, v. 23, n. 4, p. 341-347, 2008.

    Palavras-Chave: teeth; oral cavity; bacterial diseases; therapy; neodymium lasers


  • IPEN-DOC 26891

    YOSHIMURA, TANIA ; RIBEIRO, MARTHA S. . Photobiomodulation activates glucose uptake in brown adipose tissue of obese mice. Lasers in Medical Science, v. 35, n. 1, p. 280-280, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Mammal fat consists ofwhite and brown adipose tissues (WATand BAT). Although most fat is energy-storing WAT, brown adipocytes dissipate energy as heat. Obese subjects exhibit BAT hyporesponsiveness to classical stimuli as cold, hence increasing BAT thermogenic capacity could be an i n t er e s t i n g t a rget f o r t r eating obesity. Photobiomodulation (PBM) seems to be a feasible alternative to activate BAT, although it has not been applied for this purpose until now. In this study, adult C57Bl/6 mice were fed with a hyperlipidic diet (HF) during 15 weeks to induce obesity. Then, they were divided into two groups: HF and HF660. The interscapular BAT of HF660 mice was exposed to a single irradiation using a 662 ± 20 nm LED (5.7 Jcm-2 at 19 mWcm-2, delivered during 300 s). The HF group was sham-irradiated. Eutrophic animals were also used. Animals received 200 mCi of 18F-FDG and were exposed to cold for basal register of PET-CT images. After PBM or sh am treat ment, mice were once again exposed to cold before PET-CT image acquisition. Our results showed that PBM was able to activate BAT in obese mice through higher glucose uptake. In contrast, PBM and shamirradiated normal fed mice presented similar levels of glucose uptake. Taking together, our findings suggest that PBM applied to the BAT is able to promote glucose uptake by obese mice and could be an interesting approach to manage obesity and its correlated morbidities.

    Palavras-Chave: adipose tissue; glucose; metabolic diseases; mice; irradiation


  • IPEN-DOC 18835

    MENEGUZZO, DAIANE T.; LOPES, LUCIANA A.; PALLOTA, RODNEY; SOARES FERREIRA, LEILA; LOPES MARTINS, RODRIGO A.B.; RIBEIRO, MARTHA S. . Prevention and treatment of mice paw edema by near-infrared low-level laser therapy on lymph nodes. Lasers in Medical Science, v. 28, n. 3, p. 973-980, 2013.

    Palavras-Chave: lymph nodes; edema; preventive medicine; near infrared radiation; laser radiation; mice


  • IPEN-DOC 22837

    MORAES, MARCIA C.D. de ; FREITAS, ANDERSON Z. ; ARANHA, ANA C.C.. Progression of erosive lesions after Nd:YAG laser and fluoride using optical coherence tomography. Lasers in Medical Science, 2017. DOI: 10.1007/s10103-016-2075-8

    Abstract: This study aimed to use optical coherence tomography (OCT) to assess the progression of erosive lesions after irradiation with Nd:YAG laser and application of topical fluoride. One-hundred and twenty dentin samples (4 × 4 × 2 mm) obtained from bovine incisors were used. Samples were protected with acid-resistant nail varnish, with exception of a central circular area 2 mm in diameter. All samples were submitted to erosive cycles with citric acid solution 0.05M(citric acid monohydrate—C6H8O7·H2O); M= 210.14 g/mol) pH 2.3, at room temperature, for 20 min, 2×/day, throughout 20 days. After 10 days of acid challenges, lesions became visible, and each group received a different treatment (n = 15): control (without treatment), topical application of sodium fluoride 2 % for 4 min; Nd:YAG laser with different irradiation parameters (1, 0.7, and 0.5 W); and the association of fluoride with the laser parameters. OCT readouts were performed on day 01 (before the first acid challenge—OCT1), on day 05 (OCT2), day 10 (OCT3—after treatment), day 15 (OCT4), day 17 (OCT5), and day 20 (OCT6). The OCT images generated made it possible to measure the amount of tooth tissue loss over the 20 days of erosive cycle, before and after treatments, and to monitor early dentin demineralization progression. After statistical analysis, the fluoride group was observed to be the one that showed smaller loss of tissue over time. The OCT technique is promising for diagnosing and monitoring erosive lesion damage; however, further in vitro and in vivo research is needed to improve its use.

    Palavras-Chave: dentin; erosion; lasers; fluorides; tomography; in vitro; irradiation; neodymium lasers


  • IPEN-DOC 20309

    SABINO, C.P. ; GARCEZ, A.S.; NUNEZ, S.C. ; RIBEIRO, M.S. ; HAMBLIN, M.R.. Real-time evaluation of two light delivery systems for photodynamic disinfection of Candida albicans biofilm in curved root canals. Lasers in Medical Science, v. 30, n. 6, p. 1657-1665, 2015.

    Palavras-Chave: antimicrobial agents; oral cavity; fungi; candida; yeasts; bioluminescence; dentistry; therapy; photosensitivity


  • IPEN-DOC 26684

    PEREIRA, S.T. ; SILVA, C.R. ; RIBEIRO, M.S. . A single application of low-level light therapy in breast cancer-bearing mice: impact on blood cell count. Lasers in Medical Science, v. 35, n. 1, p. 274-274, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Low-level light therapy (LLLT) has been used in healthcare as a therapeutic strategy for diseases of various aetiologies. However, its effects on cancer is controversial. The aim of this work was to evaluate the effects of different light doses on blood cell count of breast cancer-bearing mice after a single application of LLLT. Tumour was induced by inoculation of 4T1 cells into the mammary fat pad of female BALB/c mice. Tumour volume was monitored with a digital calliper and when it reached 100 mm3, animals were irradiated by a red LED irradiator (660 ± 20 nm) at irradiance of 24.3 mW/cm2. The animals were randomly divided into groups (n= 5/group) that received energy densities of 1.8, 5.6 and 9.4 J/cm2. Control group was equally manipulated but did not receive irradiation. After treatment, data as tumour volume, platelets, white and red cell levels were registered weekly during 21 days. Our results showed that irradiated groups presented a longer survival compared to the control group, although no significant differences were observed among groups regarding tumour volume. Platelet levels of the irradiated groups remained closer to the reference values of healthy animals. Regarding the total number of white and red cells, no significant differences were observed between control and treated groups. These findings indicate that LLLT in a single application did not arrest tumour growth but may maintain platelet levels to increase survival of mice regardless the energy density protocol used.

    Palavras-Chave: blood cells; light emitting diodes; neoplasms; dose rates; blood platelets


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Autor: Maprelian

Título: loss of coolant

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Ano de publicação: 2015

Para indexação dos documentos é utilizado o Thesaurus do INIS, especializado na área nuclear e utilizado em todos os países membros da International Atomic Energy Agency – IAEA , por esse motivo, utilize os termos de busca de assunto em inglês; isto não exclui a busca livre por palavras, apenas o resultado pode não ser tão relevante ou pertinente.

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1. Portaria IPEN-CNEN/SP nº 387, que estabeleceu os princípios que nortearam a criação do RDI, clique aqui.

2. A experiência do Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) na criação de um Repositório Digital Institucional – RDI, clique aqui.