Navegação por Revista "Lasers in Surgery and Medicine"

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  • IPEN-DOC 11895

    GARCEZ, AGUINALDO S.; RIBEIRO, MARTHA S. ; TEGOS, GEORGE P.; NUNEZ, SILVIA C.; JORGE, ANTONIO O.C.; HAMBLIN, MICHAEL R.. Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection. Lasers in Surgery and Medicine, v. 39, p. 59-66, 2007.

    Palavras-Chave: photosensitivity; oral cavity; infectious diseases; bioluminescence; dentistry; therapy

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  • IPEN-DOC 23128

    LEAL, CINTIA; ALVARENGA, LETICIA; SILVA, TAMIRES; BUSSADORI, SANDRA; KATO, ILKA; RIBEIRO, MARTHA ; PRATES, RENATO. Application of photodynamic therapy with led and methylene blue in streptococcus mutans in the presence of glucose- in vitro study. Lasers in Surgery and Medicine, v. 49, n. S28, p. 47-47, 2017. DOI: 10.1002/lsm.22650

    Abstract: Background: Streptococcus mutans is a microorganism associated with tooth decay; many genes that express adhesion, biofilm formation, extracellular polysaccharide, carbohydrate uptake and acid tolerance regulate its cariogenic properties. S. mutans inhabits a complex biofilm and it produces a large amount of exopolysaccharides to promote adhesion and enabling transport pumps. Photodynamic therapy involves the use of a photosensitizer (PS), which is absorbed by specific cells followed by irradiation with visible light, resulting in cell death. The aim of this study was to investigate antimicrobial photodynamic therapy on Streptococcus mutans in the presence of glucose. Study: Streptococcus mutans was grown in brain heart infusion (BHI) at 37 8C for 48 h. Inocula were prepared with pure colonies, which were suspended in phosphate buffered saline (PBS) with and without 50mM glucose. One-hundred micromolar methylene blue was used as photosensitizer and the experiments were performed with groups (control, irradiated with LED, FS without irradiation, and PDT 30, 60, and 120 s). Colony form units were counted and statistically analyzed (oneway ANOVA and Tukey 5%). Results: The irradiation as well as the photosensitizer in the dark did not cause cell death. In contrast, in experiments without glucose, PDT caused cell death proportional the amount of light used. The more light, the higher the inactivation of S. mutans and after 2 min a reduction of 7 orders of magnitude (100%) was observed. In experiments with glucose cell death was observed even increasing the radiant exposure. Conclusion: We concluded that PDT is a viable solution for inactivation of S. mutans in suspension, and that the presence of glucose activates efflux pumps in the bacterial cell wall, and it drastically reduces the effect of PDT.

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  • IPEN-DOC 20914

    MORAES, MARCIA ; ARANHA, ANA ; ZEZELL, DENISE ; FREITAS, ANDERSON . Assessment of the optical attenuation coefficient of erored dentin. Lasers in Surgery and Medicine, v. 47, Suppl. 26, p. 32-33, 2015.

    Palavras-Chave: coherent radiation; attenuation; dentin; neodymium lasers; fluorine; diagnostic uses; tomography; optical models

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  • IPEN-DOC 22391

    MORAES, MARCIA; ARANHA, ANA ; ZEZELL, DENISE ; FREITAS, ANDERSON . Assessment to the optical attenuation coefficient of erored dentin. Lasers in Surgery and Medicine, v. 47, Sippl. 26, p. 32-33, 2015.

    Palavras-Chave: attenuation; dentin; in vitro; evaluation; tomography; irradiation; neodymium lasers; fluorides; yttrium compounds

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  • IPEN-DOC 20905

    LIMA, CASSIO ; GOULART, VIVIANE ; CORREA, LUCIANA ; ZEZELL, DENISE . Biochemical changes in normal skin caused by squamous cell carcinoma using FTIR spectroscopy. Lasers in Surgery and Medicine, v. 47, Suppl. 26, p. 2, 2015.

    Palavras-Chave: carcinomas; skin; biological radiation effects; fourier transform spectrometers; infrared spectra; diagnostic uses

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  • IPEN-DOC 16665

    BENETTI, CAROLINA; SANTOS, MOISES; RABELO, JOSE; ANA, PATRICIA; ZEZELL, DENISE . Characterization of irradiated bone by FTIR. Lasers in Surgery and Medicine, v. Suppl.21, p. 78-79, 2009.

    Palavras-Chave: bone tissues; chemical radiation effects; post-irradiation examination; fourier transform spectrometers; infrared spectra; surgery; laser radiation

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  • IPEN-DOC 11593

    SILVA, DANIELA F.T.; VIDAL, BENEDICTO C.; ZORN, TELMA M.T.; ZEZELL, DENISE M. ; RIBEIRO, MARTHA S. . Collagen birefrigence in skin samples following low-intensity polarized laser radiation. Lasers in Surgery and Medicine, p. p. 8, 2004.

    Palavras-Chave: skin; sample preparation; collagen; wounds; visible radiation; laser radiation; polarization

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  • IPEN-DOC 12304

    EDUARDO, FERNANDA P.; MEHMERT, DOLORES U.; MONEZI, TELMA A.; ZEZELL, DENISE M. ; SCHUBERT, MARK M.; EDUARDO, CARLOS P.; MARQUES, MARCIA M.. Cultured epithelial cells response to phototherapy with low intensity laser. Lasers in Surgery and Medicine, v. 39, p. 365-372, 2007.

    Palavras-Chave: epithelium; cell cultures; therapy; low dose irradiation; lasers; animal cells; growth

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  • IPEN-DOC 10882

    WETTER, N.U. ; BARROSO, M.C.S.; PELINO, J.E.P.. Dental bleaching efficacy with diode laser and LED irradiation: an in vitro study. Lasers in Surgery and Medicine, v. 35, p. 254-258, 2004.

    Palavras-Chave: teeth; bleaching; enamels; semiconductor lasers; light emitting diodes

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  • IPEN-DOC 11474

    CECCHINI, RENATA C.M.; ZEZELL, DENISE M. ; OLIVEIRA, ELISABETH ; FREITAS, PATRICIA M.; EDUARDO, CARLOS de P.. Effect of Er:YAG laser on enamel acid resistance: morphological and atomic spectrometry analysis. Lasers in Surgery and Medicine, v. 37, p. 366-372, 2005.

    Palavras-Chave: caries; enamels; demineralization; lasers; erbium; carbon dioxide lasers; emission spectroscopy; radiation effects

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  • IPEN-DOC 13675

    NUNEZ, SILVIA C.; RIBEIRO, MARTHA S. ; GARCEZ, AGUINALDO S.; NOGUEIRA, GESSE E.C. ; LAGE MARQUES, JOSE L.. Effects of He-Ne Laser on blood microcirculation during wound healing process. Lasers in Surgery and Medicine, v. suppl. 16, p. 4, 2004.

    Palavras-Chave: blood flow; flowmeters; therapy; skin; repair; lasers; doppler effect

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  • IPEN-DOC 16400

    ZEZELL, DENISE ; ANA, PATRICIA; ALBERO, FELIPE; BACHMANN, LUCIANO. Effects of lasers on chemical composition on enamel and dentin. Lasers in Surgery and Medicine, v. Suppl.21, p. 53, 2009.

    Palavras-Chave: enamels; dentin; laser radiation; radiation effects; morphological changes

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  • IPEN-DOC 10883

    CRUZ, D.R.; KOHARA, E.K.; RIBEIRO, M.S.; WETTER, N.U. . Effects of low-intensity laser therapy on the orthodontic movement velocity of human teeth: a preliminary study. Lasers in Surgery and Medicine, v. 35, n. 2, p. 117-120, 2004.

    Palavras-Chave: teeth; orthodontic treatments; semiconductor lasers; light emitting diodes; therapy; bone tissues

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  • IPEN-DOC 24853

    MOSCA, RODRIGO C. ; SANTOS, SOFIA N. ; NOGUEIRA, GESSE E.C.; PEREIRA, DAISA L. ; COSTA, FRANCIELLI C.; ARANY, PRAVEEN; ZEITUNI, CARLOS A. . Efficacy of photobiomodulation therapy in mitigating skin radiation damage. Lasers in Surgery and Medicine, v. 50, 29, p. S15-S16, 2018. DOI: 10.1002/lsm.22799

    Abstract: Background: The use of sophisticated radiation dose delivery and fractionation has significantly improved cancer care. One of these involves localized, sustained ionizing dose delivery termed brachytherapy. Despite it therapeutic efficacy, specific side effects of brachytherapy include localized skin damage and breakdown for which only palliative treatments are currently available. The use of low dose biophotonics treatments to promote tissue healing is termed photobiomodulation (PBM) therapy. The aim of this study was to evaluate efficacy and molecular pathways of PBM therapy using two common wavelengths, red and near-infrared (NIR) to treat radiation wounds in athymic mice subjected to brachytherapy (sustained ionizing radiation from 125I seeds). Study Design/Materials and Method: A pilot study was performed with thirty-six athymic mice were accomplished for 60 days and divided into six groups: Surgical Control Group (No radiation and no PBM treatments); Radiation Control Group (125I seed 0.4252 mCi, no PBM); NIR-PBM Control Group (NIR PBM alone, LED at l¼880 nm); Red-PBM Control Group (Red PBM alone LED at l¼660 nm); Radiation- NIR PBM Group; Radiation-Red PBM Group. Following 21 days, radiation-induced wounds are evident. PBM treatments (both wavelengths with output power 40mW for 20 s, fluence 20 J/cm2 on top of implantation site) were performed every week up to 60 days. Wounds were evaluated every 7 days digital imaging, Laser Doppler Flowmetry (LDF) and tissue temperature with a thermographic camera. We also performed mPET-CT imaging using radioactive fluorodeoxyglucose (18F-FDG) at 51 and 81 days post-implantation. Animals were sacrifices progressively at each time point to correlate clinical observations with imaging and molecular tissue analyses. Tissues were collected to analyze molecular pathways correlating with inflammation, immune response, wound healing and angiogenesis using mRNA (qRT-PCR) and protein expression (immunostaining). Results: Both PBM treated groups demonstrated significant (p<0.05) improvements in skin radiation wound healing as compared to radiation group. Distinct improvements in clinical wound size and closure, improved tissue perfusion and reduced inflammation as evidenced by decreased wound thermal images. These wounds were also noted to have significant differences in the cytokine profiles (TGF-b, VEGF and PDGF) correlating with better healing responses. Radiation damage reduces brown fat composition that can potentially contribute to additional radiation-associated morbidities. The mPET-CT imaging noted significant preservation of brown fat composition in PBM-treated radiation alone groups. Further validation of these pathways is ongoing. Conclusion: Within the parameters of this study, PBM treatments demonstrated improved healing in radiation wounds due to ionizing radiation from 125I seeds. Ongoing work is examining the precise molecular pathways contributing to these therapeutic benefits. It is hoped this study will enable further development of this innovative therapy for managing side- effects from radiation treatments.

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  • IPEN-DOC 20253

    CARA, ANA C.B. ; ZEZELL, DENISE M. ; ANA, PATRICIA A.; MALDONADO, EDISON P. ; FREITAS, ANDERSON Z. . Evaluation of two quantitative analysis methods of optical coherence tomography for detection of enamel demineralization and comparison with microhardness. Lasers in Surgery and Medicine, v. 46, n. 9, p. 666-671, 2014.

    Palavras-Chave: teeth; caries; enamels; demineralization; coherent radiation; quantitative chemical analysis; tomography; optical models

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  • IPEN-DOC 19868

    RIBEIRO, MARTHA; KATO, ILKA; SABINO, CAETANO; YOSHIMURA, TANIA; HAMBLIN, MICHAEL; PRATES, RENATO. Evaluation of variables involved in antifungal photodynamic therapy. Lasers in Surgery and Medicine, v. 45, p. 62, 2013.

    Palavras-Chave: mice; female genitals; candida; fungi; antimicrobial agents; in vitro; photosensitivity; therapy

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  • IPEN-DOC 16401

    ZEZELL, DENISE M. ; ANA, PATRICIA A.; BENETTI, CAROLINA; QUINTO JUNIOR, JOSE; PEREIRA, THIAGO M.; GOULART, VIVIANE. FTIR spectroscopy monitoring chemical changes in enamel dentin and bone due to laser irradiation. Lasers in Surgery and Medicine, v. Suppl.22, p. 47-48, 2010.

    Palavras-Chave: teeth; enamels; dentin; bone tissues; laser radiation; radiation effects; fourier transform spectrometers

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  • IPEN-DOC 10832

    NUNEZ, S.C.; NOGUEIRA, G.E.C. ; RIBEIRO, M.S.; GARCEZ, A.S.; MARQUES, J.L.L. He-Ne laser effects on blood microcirculation during wound healing: a method of in vivo study through laser doppler flowmetry. Lasers in Surgery and Medicine, v. 35, n. 5, p. 363-368, 2004.

    Palavras-Chave: blood flow; flowmeters; therapy; skin; repair; lasers; doppler effect

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  • IPEN-DOC 22393

    SILVA, TAMIRES O.; SUZUKI, LUIS; KATO, ILKA ; DEANA, ALESSANDRO; RIBEIRO, MARTHA ; PRATES, RENATO . In vitro effect photodynamic therapy with LED and methylene blue on Candida albicans pretreated with glucose. Lasers in Surgery and Medicine, v. 48, Suppl. 27, p. 53-54, 2016.

    Palavras-Chave: in vitro; therapy; candida; light emitting diodes; methylene blue; glucose; irradiation; biological effects

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  • IPEN-DOC 23233

    SILVA, TAMIRES; ALVARENGA, LETICIA; LEAL, CINTIA; MIRANDA, BIANCA G.; SUZUKI, LUIZ C.; KATO, ILKA ; RIBEIRO, MARTHA ; PAVANI, CHRISTIANE; PRATES, RENATO . Incorporation of the methylene blue photosensitizer in the presence of glucose in strains of candida albicans with multidrug efflux systems. Lasers in Surgery and Medicine, v. 49, n. 4, p. 438-438, 2017.

    Abstract: Background: Infectious diseases are responsible for 60% of deaths in developing countries.Candida albicans cause severe infection in immunocompromised patients and the commonly used treatments have not been effective against microbial resistance. Photodynamic therapy (PDT) is a new promising strategy to microbial inactivation based on the use of photosensitizer (PS) in the presence of oxygen and activation by light to form reactive oxygen species.C.albicanshas glucose sensors which could facilitate the entrance of the PS. The objective of this study was to evaluate the role of glucose in the photodynamic inactivation of C. albicans. Study: C. albicans ATCC 10231, YEM 12, YEM 13, YEM 14, and YEM15were aerobically grown on Sabouraud agar and incubated at 308C for 24 hours. Microbial inoculants were divided into 4 groups with and without glucose: Control; Only irradiation; PS toxicity and PDT groups with three irradiation times.After going throughthe treatments, the colony-forming units were counted and the data were subjected to statistical analysis (ANOVA) and Tukey test. To measure the concentration ofMB, fluorescence spectroscopy and flow cytometry were used. Results: We observed that yeast with overexpression of Major Facilitator Superfamily membrane pore tend to accumulate more MB in its cytoplasm, whereas strains that overexpress ABC pumps demonstrated the greater survival against the photodynamic challenge. Conclusion: PDT is an efficient strategy against C. albicans and the presence of glucose can intervene in the photodynamic effect. The presence of MB in the intracellular region of the yeast predisposes to greater photodynamic inactivation, however, its germicidal capacity is related to the type and characteristics of the microbial.

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1. Portaria IPEN-CNEN/SP nº 387, que estabeleceu os princípios que nortearam a criação do RDI, clique aqui.


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