Navegação IPEN por Autores IPEN "AFFONSO, REGINA"

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  • IPEN-DOC 24465

    ARAUJO, RENATO M.; AFFONSO, REGINA ; SILVA, JOSIAS L. ; BELLINI, MARIA H. . Adenocarcinoma prostático: análise clínica e epidemiológica / Prostate adenocarcinoma: clinical and epidemiological analysis. Revista da Sociedade Brasileira de Clínica Médica, v. 15, n. 03, p. 178-182, 2017.

    Abstract: OBJETIVO: Realizar análise clínica e epidemiológica de pacientes com câncer de próstata. MÉTODOS: Estudo retrospectivo, descritivo de 607 prontuários de pacientes com câncer de próstata, atendidos entre 2012 a 2014. As variáveis analisadas foram: procedência, faixa etária, antígeno prostático específico (PSA) total, escore de Gleason da biópsia e da peça cirúrgica. A análise estatística foi realizada com software SPSS, versão 19.0. RESULTADOS: A maioria dos pacientes (57%) era de Ipatinga (MG) e arredores. A faixa etária mais frequente foi de 61 a 80 anos (76,6%). Valores de PSA entre 4,1 a 10ng/mL foram mais frequentes. O escore de Gleason da biópsia revelou que 321 pacientes apresentavam tumor intermediário. Apenas 203 pacientes realizaram a prostectomina, e 61,5% das peças cirúrgicas também apresentaram tumor intermediário. Houve correlação significativa entre as faixas etárias e os níveis de PSA (R2=0,9319), e também entre o nível de PSA e os valores de escore Gleason da biópsia (p<0,05). Houve concordância entre os valores de escore de Gleason da biópsia com os da peça cirúrgica em 72,9% dos casos. CONCLUSÃO: Em nosso conhecimento, este foi o primeiro estudo epidemiológico de câncer de próstata na região do Vale do Aço. As informações fornecidas neste trabalho podem contribuir com programas para desenvolver ações de controle do câncer de próstata nesta região.

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  • IPEN-DOC 21836

    SARAIVA, KATHERINE W. ; AFFONSO, REGINA . Análise da proína recombinante RPL-10 nativa expressa em sistema bacteriano. In: PROGRAMA INSTITUCIONAL DE BOLSAS DE INICIAÇÃO CIENTÍFICA, 18.; PROGRAMA DE BOLSAS E INICIAÇÃO CIENTÍFICA CNEN, 9.; PROGRAMA INSTITUCIONAL DE BOLSAS DE INICIAÇÃO DESENVOLVIMENTO TECNOLÓGICO E INOVAÇÃO, 2., 24-25 de outubro, 2012, São Paulo, SP. Resumo expandido... 2012.

    Palavras-Chave: proteins; bacteria; fluorescence spectroscopy; electrophoresis; animal cells; growth; genetic engineering

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  • IPEN-DOC 26592

    SANTOS, CAROLINA M. dos ; AFFONSO, REGINA . Caracterização do sítio catalítico da enzima conversora de angiotensina-I região N-domínio. In: PROGRAMA INSTITUCIONAL DE BOLSAS DE INICIACAO CIENTIFICA E TECNOLOGICA; SEMINARIO ANUAL PIBIC, 25.; SEMINARIO ANUAL PROBIC, 16.; SEMINARIO ANUAL PIBITI, 9, 6-7 de novembro, 2019, São Paulo, SP. Resumo expandido... São Paulo: IPEN-CNEN/SP, 2019. p. 100-101.

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  • IPEN-DOC 06917

    AFFONSO, REGINA . Estudo da expressao citoplasmica bacteriana de uma forma de prolactina humana e de sua solubilizacao e renaturacao a partir de corpos de inclusao. 2000. Tese (Doutoramento) - Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP, Sao Paulo. 105 p. Orientador: Paolo Bartolini.

    Palavras-Chave: lth; escherichia coli; cytoplasm; bacteriophages; recombinant dna; separation processes; solubility; purification; hormones; genes; bacteria

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  • IPEN-DOC 24637

    TARGINO, BARBARA ; PINTO, THAIS L. ; SILVA, EVILY F. ; SOMESSARI, E.S.R. ; BELLINI, MARIA H. ; AFFONSO, REGINA . Evaluation of low doses of gamma irradiation in the formation of mineralization nodules in osteoblasts culture. In: ANAIS DA SOCIEDADE BRASILEIRA DE BIOCIENCIAS NUCLEARES, 09-11 de outubro, 2017, São Paulo, SP. Abstract... Rio de Janeiro, RJ: Sociedade Brasileira de Biociências Nucleares, 2017. p. 116-116.

    Abstract: Introduction: Osteoblasts are specialized fibroblasts that secrete and mineralize the bone matrix. The mineralized extracellular matrix is mainly composed of type I collagen, osteocalcin, and the inorganic mineral hydroxylapatite1. The use of radiation as therapy in some cancers causes great bone loss. However, low dose radiation may have the opposite effect. Low dose X-irradiation on osteoblastic culture had effects on proliferation and differentiation with increase of mineralization nodules2. However, there is little information on the potential therapeutic efficacy of low-dose gamma-irradiation in the formation of mineralization nodules. Objective: To evaluate the effects of irradiation with 60Co γ-rays in low doses in the formation of mineralization nodules in culture of osteoblasts. Methods: MC3T3-E1 cells were bought by the Banco de Células do Rio de Janeiro, Brazil (MC3T3-E1 Subclone 14). The cells were cultured in α-MEM medium consisting of 10% FBS and without β-glycerophosphate and L-ascorbic acid (GIBCO, Custom Product, Catalog No. A1049001) (Zhao Y, Guan H, Liu S et al. Biol. Pharm. Bull. 2005, 28(8):1371-1376). Plating efficiency assays: cells were plated at a density of 100 cell/plate into 60 mm Petri dishes. After 14 days the places were stained with violet crystal and the colonies were counted. -glycerophosphate and 50 mg/ml ascorbic acid, and analyzed on days 7, 14 and 21. Osteoblast culture irradiation assay: cells were plated at a density of 1x 105 cells/plate on 60 mm dishes and the next day were irradiated by 60Co source with 0 (as the control), 0.5, 1.0, 1.5 and 2.0 Gy using the GammaCell 220 – Irradiation Unit of Canadian-Atomic Energy Commission Ltd. (CTR-IPEN). On day 21 of culture, undifferentiated (without ascorbic acid and β-glycerophosphate), differentiating cells (0 Gy) and irradiated cells at different doses, the medium was removed, cells were washed with phosphate buffer saline, fixed with 70% ethyl alcohol and stained with Alizarin red S (Sigma). All in three biological replicates (a total of 54 samples) and multiple comparisons were assessed by One-way ANOVA followed by Bonferroni´s tests with GraphPad Prism version 6.0 software. P< 0.05 was considered statistically significant. Results: Plating efficiency (PF) analysis is generally considered to be the gold standard of assays for testing the sensitivity of cell lines to ionizing radiation or other cytotoxic agents in vitro. The results obtained were a PF of 30% for non-irradiated culture, however, the irradiated culture obtained 40% in relation to the no-irradiated one, already with 0.5 Gy, and this percentage was maintained in the other larger doses. Regarding the evaluation of the formation of mineralization nodules, significant difference in 0.5 Gy group was observed compared with the control group (0 Gy), 64.7±1.8 and 53.0±0.9, respectively. The groups of 1.0, 1.5 and 2.0 Gy obtained a decrease in the mineralization nodules. The data obtained with increasing irradiation produced an increase of mineralization nodules up to 0.5 Gy and in the higher doses had a decrease. Applying the data in a non-linear function it is observed that the line has a decreasing tendency with the negative angular coefficient. This analysis is in agreement with the hormesis model, in which low doses induce a stimulatory effect while high doses cause inhibition4. Conclusions: This study is one among the first that investigating the biophysics of low-dose gamma-irradiation on MC3T3-E1 culture, focusing on the potential applications in bone replacement therapy.

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  • IPEN-DOC 24265

    SILVA, FELIPE D. ; SUZUKI, MIRIAM ; OLIVEIRA, JOAO ; FREIRE, RENAN ; BARTOLINI, PAOLO ; SOARES, CARLOS ; AFFONSO, REGINA . Expression of human prolactin in HEK293T using different transfection reagents. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 46th, July 27-30, 2017, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular, 2017.

    Abstract: INTRODUCTION Prolactin is a hormone produced by the pituitary gland with numerous functions, such as lactation, reproduction, osmotic and immune regulation. This hormone is upregulated in cases of lack of lactation, infertility and cancer. Recombinant prolactin has been produced in Escherichia coli with an initial methionine which may cause immunological reactions, or in its authentic form in mammalian cells. Our laboratory has already synthesized human prolactin (hPRL) without initial methionine in E. coli periplasm and Chinese hamster ovary cells. CHO cells have been widely used in the synthesis of human recombinant proteins because of their similarity with human post-translational modifications as glycosylation. The HEK293, a human embryonic kidney cell, can do diverse glycosylation depend on culture conditions. OBJECTIVES This work compares different transfection reagents in the production of hPRL in HEK293T. MATERIALS AND METHODS The hPRL cDNA was inserted into the pEDdc vector donated by the Genetics Institute, USA. Three transfection reagents were used: LipofectamineTM (Thermo), XfectTM (Clontech), and ExpiFectamineTM (Thermo). HEK293T cells, a human strain, were cultured in 10 cm² Ø petri dishes with RPMI 1640 medium with 10% fetal bovine serum (FBS). After transfection, the medium was changed to serum free CHO-S-SFM II (Invitrogen, USA). 100% of the medium was collected and changed every two days. The collected medium was stored at -80°C. Samples were analyzed by SDS-PAGE, Western blotting and HPLC. DISCUSSION AND RESULTS The glycosylated and non-glycosylated hPRL forms secreted into the culture medium were confirmed by Western blot and RPHPLC in the three transfected cultures in recombinant human cells. The reagent with the best result was Xfect (2 μg/mL), followed by Lipofectamine (1.6 μg/mL) and Expifectamine (1.2 μg/mL). CONCLUSION The transient expression of hPRL using HEK293T cells enable laboratory production of glycosylated hPRL for future studies of N-glycans produced by these cells.

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  • IPEN-DOC 12742

    COSTA, ANA M.M.; SILVA, ISMAEL D.C.G. da; AFFONSO, REGINA ; SOARES JUNIOR, JOSE M.; NUNES, MAIRA G. ; LIMA, GERALDO R. de; BARACAT, EDMUND C.. Gene analysis in patients with premature ovarium failure or gonadal dysgenesis: A preliminary study. Maturitas, v. 57, n. 4, p. 399-404, 2007.

    Palavras-Chave: genes; ovaries; failures; gene mutations; gonads

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  • IPEN-DOC 25134

    RICCI, GIANNINA; SANTOS, DANIEL W.; KOVACS, JOSEPH A.; NISHIKAKU, ANGELA S.; SANDES-FREITAS, TAINA V. de; RODRIGUES, ANDERSON M.; KUTTY, GEETHA; AFFONSO, REGINA ; SILVA, HELIO T.; MEDINA-PESTANA, JOSE O.; FRANCO, MARCELLO F. de; COLOMBO, ARNALDO L.. Genetic diversity of Pneumocystis jirovecii from a cluster of cases of pneumonia in renal transplant patients: Cross-sectional study. Mycoses, v. 61, n. 11, p. 845-852, 2018. DOI: 10.1111/myc.12823

    Abstract: Pneumocystis jirovecii can cause severe potentially life-threatening pneumonia (PCP) in kidney transplant patients. Prophylaxis of patients against PCP in this setting is usually performed during 6 months after transplantation. The aim of this study is to describe the molecular epidemiology of a cluster of PCP in renal transplant recipients in Brazil. Renal transplant patients who developed PCP between May and December 2011 had their formalin-fixed paraffin-embedded (FFPE) lung biopsy samples analysed. Pneumocystis jirovecii 23S mitochondrial large subunit of ribosomal RNA (23S mtLSU-rRNA), 26S rRNA, and dihydropteroate synthase (DHPS) genes were amplified by polymerase chain reaction (PCR), sequenced, and analysed for genetic variation. During the study period, 17 patients developed PCP (only four infections were documented within the first year after transplantation) and six (35.3%) died. Thirty FFPE samples from 11 patients, including one external control HIV-infected patient, had fungal DNA successfully extracted for further amplification and sequencing for all three genes. A total of five genotypes were identified among the 10 infected patients. Of note, four patients were infected by more than one genotype and seven patients were infected by the same genotype. DNA extracted from FFPE samples can be used for genotyping; this approach allowed us to demonstrate that multiple P. jirovecii strains were responsible for this cluster, and one genotype was found infecting seven patients. The knowledge of the causative agents of PCP may help to develop new initiatives for control and prevention of PCP among patients undergoing renal transplant and improve routine PCP prophylaxis.

    Palavras-Chave: kidneys; transplants; pneumonia; diseases; genetics; epidemiology; patients; immune system diseases

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  • IPEN-DOC 25046

    AFFONSO, REGINA ; SOARES, CARLOS R. ; RIBELA, MARIA T. ; BARTOLINI, PAOLO . High production and optimization of the method for obtaining pure recombinant human prolactin. Protein Expression and Purification, v. 152, p. 131-136, 2018. DOI: 10.1016/j.pep.2018.07.015

    Abstract: Prolactin is a pituitary hormone that is involved diverse physiological functions, such as lactation, reproduction, metabolism, osmoregulation, immunoregulation, and behavior. Its level of glycosylation is low in vivo, which favors its expression in bacterial systems. In the present work recombinant human prolactin (rec-hPRL) was expressed from the p1813-hPRL vector in Escherichia coli strain in inclusion bodies with 530.67 mg of rec-hPRL per liter of induced bacterial culture. The solubilization and renaturation of rec-hPRL followed by two methods described in the literature for this protein: one with detergent and basic pH, and other urea and dialyses was done by studying. The protocol with detergent/basic pH was not successful, whereas protocol with urea/dialyses was obtained pure protein and this was optimized. Rec-hPRL was obtained in a soluble, pure and active form, when the sample was 8-fold concentrated in the solubilization phase, allowing 33% recovery, 3-fold more that the original method. The pure protein was obtained with 38.37 i. u./mg activity, which is three times greater than that of the PRL standard from the WHO. In conclusion, this work obtained the highest production of rechPRL, and concentrating the sample eight times in the solubilization stage was decisive for obtaining a highly concentrated, active protein for future work.

    Palavras-Chave: lth; hormones; biological effects; bacteria; proteins; in vitro; bioassay; statistical data; lactogens; pituitary hormones; biotechnology

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  • IPEN-DOC 17399

    OGUIURA, NANCY; MITAKE, MALVINA B. ; AFFONSO, REGINA ; ZHANG, GUOLONG. In vitro antibacterial and hemolytic activities of crotamine, a small basic myotoxin from rattlesnake Crotalus durissus. Journal of Antibiotics, v. 64, n. 4, p. 327-331, 2011.

    Palavras-Chave: antimicrobial agents; peptides; hemolysis; escherichia coli; snakes; venoms; toxins

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  • IPEN-DOC 25985

    SANTOS, CAROLINA M. dos ; FIGUEIREDO, ALINE M. de ; LEITE, RODRIGO C. ; CAMARGO, NATANE M. de; AFFONSO, REGINA . A new approach to obtain the catalytic site of human Angiotensin Converting Enzyme. In: EUROPEAN SYMPOSIUM ON BIOCHEMICAL ENGINEERING SCIENCES, 12th, September 9-12, 2018, Lisbon, Portugal. Abstract... Frankfurt am Main, Germany: European Society of Biochemical Engineering Sciences - ESBES, 2018.

    Abstract: Angiotensin-converting enzyme I (ACE) is a key part of the renin-angiotensin system whose main function is to regulate blood pressure. The sACE possesses two domains, N- C-, with catalytic sites which exhibit 60% sequence identity. These domains differ in terms of chloride-ion activation profiles, rates of peptide hydrolysis of angiotensin I, bradykinin, angiotensina (1-7), beta-amyloid peptide and sensitivities to various inhibitors. A more detailed analysis shows that these regions are composed of HEMGH and EAIGD sequences, which are the catalytic sites. Our question is: If the synthesis of catalytic sites with corrects structure and activity could be a good model per si to study new drugs. In our laboratory the catalytic site of the C-domain was obtained with correct structural conformation and with enzymatic activity. The objective this work is to obtain the Ala361 to Gli468 catalytic site, N-domain, in a structural conformation that resembles its native form. The 380 pb cDNA to catalytic site was cloned in the pE1 vector (kindly provided by Dr. David Wood), elastinlike-polyptide (ELP) tag sequence was linked with catalytic site, ELP~csACEn recombinant protein, this was expressed in bacteria with Terrificus broth with 0.1 mM IPTG for 20h. Harvested cells were resuspended in TE buffer, after sonication and centrifugation the pellet was resuspended the same buffer. The ELP~csACEn was precipitated with 0.8 M ammonium sulfate and the tag sequence was cleaved by pH change, pH 6.2. All steps were analyzed by SDS-PAGE, Dot and Western blotting. The catalytic site was synthesized from bacterial system with ELP sequence tag in soluble form. The purification process was done by ammonium sulfate precipitation and cleavage of the ELP sequence by changing to acidic pH. The characterization of catalytic site by SDS-PAGE shows that this is pure and Western blotting immunological assay confirmed the identity of the protein as csACEn. The strategy used to obtain the Ala361 to Gli468 catalytic site in soluble and pure form was successful. The next steps: we will continue with the Maldi-tof and structural conformation analyzes.

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  • IPEN-DOC 26944

    AFFONSO, REGINA ; SAMPAIO, SUELEN de B. ; JANUARIO, FAGNER S. ; PEREIRA, LARISSA M.; ARAGÃO, DANIELLE S.; CASARINI, DULCE E.; ELIAS, CAROLINE C. . A new approach to obtain the catalytic sites region of human sACE with correct fold and activity. Journal of Biotechnology and Biomaterials, v. 7, n. 1, p. 96-96, 2017. DOI: 10.4172/2155-952X.C1.071

    Abstract: Angiotensin-converting enzyme I (ACE) is a membrane-bound that catalyzes the conversion of angiotensin I to the potent vasopressor angiotensin II. ACE is a key part of the renin-angiotensin system, which regulates blood pressure and is widely distributed throughout the body. There are two isoforms of human ACE, including the somatic ACE (sACE) present in somatic tissue and the testicular ACE (tACE) present in male germinal cells. The sACE possesses two domains, N- C- domains, with catalytic sites which exhibit 60% sequence identity. These domains differ in terms of chloride-ion activation profiles, rates of peptide hydrolysis of angiotensin I, bradykinin, Goralatide, Luliberin, substance P, angiotensina, beta-amyloid peptide and sensitivities to various inhibitors. A more detailed analysis shows that these regions are composed of HEMGH and EAIGD sequences that bind zinc ions to facilitate catalytic activity (Fig. 1). Our question is: If the synthesis of catalytic sites with corrects structure and activity could be a good model per si to study new drugs. The objective was to obtain the Ala361 a Gli468 and Ala959 to Ser1066 catalytic regions sACE in a structural conformation that resembles its native form. The catalytic regions were obtained from bacterial system; the expression of this protein in soluble form enables completion of the solubilization/purification steps without the need for refolding. The characterization of Ala959 to Ser1066 region shows that this has an α-helix and β-strand structure, Fig. 1b, which zinc ion (essential for its activity) binds to, and with enzymatic activity. Our conclusion is that the strategy used to obtain the Ala959 to Ser1066 region in the correct structural conformation and with activity was successful.

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  • IPEN-DOC 13750

    CHURA-CHAMBI, ROSA M.; GENOVA, LUIS A. ; AFFONSO, REGINA ; MORGANTI, LIGIA . Refolding of endostatin from inclusion bodies using high hydrostatic pressure. Analytical Biochemistry, v. 379, n. 1, p. 32-39, 2008.

    Palavras-Chave: escherichia coli; proteins; hydrostatics; bacteria; inclusions; agglomeration; collagen; endothelium

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  • IPEN-DOC 20242

    PEREIRA, LARISSA M. ; SILVA, LUANA R. ; ALVES, JOSEANE F. ; MARIN, NELIDA; SILVA, FLAVIO S. ; MORGANTI, LIGIA ; SILVA, ISMAEL D.C.G.; AFFONSO, REGINA . A simple strategy for the purification of native recombinant full-length human RPL10 protein from inclusion bodies. Protein Expression and Purification, v. 101, p. 115-120, 2014.

    Palavras-Chave: ribosomes; messenger-rna; cloning; carcinomas; gene therapy; inclusions; folding model; tumor cells; inhibition; fluorescence spectroscopy

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  • IPEN-DOC 16093

    ANDRADE, MARIA C.C. de; AFFONSO, REGINA ; FERNANDES, FERNANDA B.; FEBBA, ANDREIA C.; SILVA, ISMAEL D.C.G. da; STELLA, REGINA C.R.; MARSON, ODAIR; JUBILUT, GUITA N.; HIRATA, IZAURA Y.; CARMONA, ADRIANA K.; CORRADI, HAZEL; ACHARYA, K.R.; STURROCK, EDWARD D.; CASARINI, DULCE E.. Spectroscopic and structural analysis of somatic and N-domain angiotensin I-converting enzyme isoforms from mesangial cells from Wistar and spontaneously hypertensive rats. International Journal of Biological Macromolecules, v. 47, n. 2, p. 238-243, 2010.

    Palavras-Chave: angiotensin; enzyme inhibitors; antihypertensive agents; dichroism; spectroscopy; rats

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  • IPEN-DOC 24238

    ELIASA, CAROLINE C. ; PEREIRA, LARISSA M. ; ARAGAO, DANIELLE S.; CASARINI, DULCE E.; AFFONSO, REGINA . Structural characterization and enzymatic activity of the recombinant Ala959 to Ser1066 region of human ace. Jacobs Journal of Enzymology and Enzyme Engineering, v. 3, n. 1, p. 1-13, 2017.

    Abstract: Angiotensin-converting enzyme catalyzes the conversion of angiotensin I to the vasoconstrictor angiotensin II and the hydrolysis of bradykinin (BK). Human somatic angiotensin-converting enzyme has two homologous domains (N and C) that share 60% identity. Although these two regions have high homology, the catalytic site of the C-domain exhibits three-fold greater activity than the N-domain in the hydrolysis of angiotensin I in vivo. The present study aimed to obtain the Ala959 to Ser1066 catalytic region of the C-domain of angiotensin-converting enzyme in a structural conformation that resembles its native form. We amplified the 324-bp sequence corresponding to the catalytic site of C-domain of angiotensin-converting enzyme and cloned this sequence into a pET28 vector. The catalytic site of C-domain of angiotensin-converting enzyme peptide was expressed in a bacterial system, and its purification was performed in one step using a His-tag affinity column. Structural analysis by circular dichroism and fluorescence confirmed that the purified protein is correctly folded, and catalytic site of C-domain of angiotensin-converting enzyme possesses enzymatic activity and is inhibited by lisinopril. This peptide can be used to test new inhibitors and C-domain of angiotensin-converting enzyme substrates because this peptide is easy to produce and this has proven efficient link with these molecules.

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  • IPEN-DOC 26714

    LINARDI, MARCELO ; NETTO, ANA P.F.A. ; LUGAO, ADEMAR B. ; FREITAS, ANDERSON Z. de ; CARBONARI, ARTUR W. ; ZEITUNI, CARLOS A. ; SOARES, CARLOS R.J. ; SILVA, CECILIA C.G. e ; ZAMBONI, CIBELE B. ; PERONI, CIBELE N. ; VIEIRA, DANIEL P. ; ANDRADE, DELVONEI A. de ; ZEZELL, DENISE M. ; LAZAR, DOLORES R.R. ; PARRA, DUCLERC F. ; MOREIRA, EDSON G. ; LANDULFO, EDUARDO ; FONSECA, EDVALDO R.P. da ; PERINI, EFRAIN A. ; ARAUJO, ELAINE B. de ; MUCCILLO, ELIANA N. dos S. ; CARVALHO, ELITA F.U. de ; BERNARDES, EMERSON S. ; MOURA, ESPERIDIANA A.B. de ; SILVA, FABIANA M. da ; MOREIRA, FERNANDO J.F. ; SILVA, FLAVIA R. de O. ; GENEZINI, FREDERICO A. ; ALVES, GLAUCIE J. ; YORIYAZ, HELIO ; COSTA, ISOLDA ; MENGATTI, JAIR ; ROSSI, JESUALDO L. ; OLIVEIRA, JOAO E. de ; SARKIS, JORGE E. de S. ; PERROTTA, JOSE A. ; ROGERO, JOSE R. ; SHORTO, JULIAN M.B. ; SILVA, LEONARDO G. de A. e ; MOLNARY, LESLIE de ; RODRIGUES, LETICIA L.C. ; DIAS, LIGIA E.M.F. ; CALDAS, LINDA V.E. ; POZZO, LORENA ; GENOVA, LUIS A. ; MATSUDA, MARGARETH M.N. ; HAMADA, MARGARIDA M. ; FELINTO, MARIA C.F. da C. ; POTIENS, MARIA da P.A. ; ROSTELATO, MARIA E.C.M. ; MARUMO, MARIA H.B. ; VASCONCELLOS, MARINA B.A. ; RIBEIRO, MARTHA S. ; COTRIM, MARYCEL E.B. ; NEVES, MAURICIO D.M. das ; MORALLES, MAURICIO ; DIAS, MAURO da S. ; IGAMI, MERY P.Z. ; DURAZZO, MICHELANGELO ; SUZUKI, MIRIAM F. ; SAIKI, MITIKO ; MATHOR, MONICA B. ; VIEIRA JUNIOR, NILSON D. ; BARTOLINI, PAOLO ; SPENCER, PATRICK J. ; SILVA, PAULO S.C. da ; AFFONSO, REGINA ; CARNEIRO, REGINA C.G. ; ROGERO, SIZUE O. ; SAKATA, SOLANGE K. ; CASTANHO, SONIA R.H. de M. ; MAIHARA, VERA A. ; ROSSI, WAGNER de ; CALVO, WILSON A.P. ; KODAMA, YASKO . O IPEN e a saúde. São Paulo, SP: SENAI-SP Editora, 2019. 280 p.

    Palavras-Chave: radiopharmaceuticals; radioactive materials; laser radiation; nuclear medicine; radiotherapy; diagnosis; environmental policy; rmb reactors; reactors

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ATENÇÃO!

ESTE TEXTO "AJUDA" ESTÁ SUJEITO A ATUALIZAÇÕES CONSTANTES, A MEDIDA QUE NOVAS FUNCIONALIDADES E RECURSOS DE BUSCA FOREM SENDO DESENVOLVIDOS PELAS EQUIPES DA BIBLIOTECA E DA INFORMÁTICA.

O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.

A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

1. Portaria IPEN-CNEN/SP nº 387, que estabeleceu os princípios que nortearam a criação do RDI, clique aqui.


2. A experiência do Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) na criação de um Repositório Digital Institucional – RDI, clique aqui.