Effects of ionizing radiation on proteins in lyophilized or frozen demineralized
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2016
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Revista Brasileira de Ortopedia
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The aim was to study the effects of application of ionizing radiation (gamma andelectrons) as sterilizing agents at doses of 15 kGy, 25 kGy and 50 kGy, on lyophilized or frozendemineralized bone tissue for use in transplants.Methods: Five human femoral diaphyses from different donors of musculoskeletal tissuewere demineralized and preserved as lyophilized or frozen at −80◦C. The samples weredivided into two groups: non-irradiated (control) and irradiated by means of gamma rays oran electron beam. The bone proteins were extracted and used to determine the concentra-tions of total protein and BMP 2 and 7.Results: Decreases in total protein and BMP 2 and 7 concentrations were observed. Thedecreases in total protein concentrations, in comparison with the respective control groups,were significant in the lyophilized and frozen samples that were irradiated at a dose of50 kGy of gamma radiation and electron beam, with reductions of more than 30%. Signifi-cant decreases in the levels of BMP 2 and 7 were also observed at higher doses and especiallythrough use of the electron beam.Conclusion: The reductions in the concentrations of total proteins and osteoinductive pro-teins (BMP 2 and 7) were related to the radiation dose, i.e. they increased with higher dosesof ionizing radiation type and the type of bone preservation. The largest reductions in con-centrations were observed in the bones irradiated by means of an electron beam and at adose of 50 kGy. However, this type of radiation and this high dose are not usual practices forsterilization of bone tissue
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ANTEBI, URI; MATHOR, MONICA B.; SILVA, ANDRE F. da; GUIMARAES, RODRIGO P.; HONDA, EMERSON K. Effects of ionizing radiation on proteins in lyophilized or frozen demineralized. Revista Brasileira de Ortopedia, v. 51, n. 2, p. 224-230, 2016. DOI: 10.1016/j.rboe.2016.02.006. Disponível em: http://repositorio.ipen.br/handle/123456789/26772. Acesso em: 19 Apr 2024.
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