The deficiency of galectin-3 in stromal cells leads to enhanced tumor growth and bone marrow metastasis
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2016
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BMC Cancer
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Background: Galectin-3 is a multifunctional β-galactoside-binding lectin that once synthesized, is expressed in the
nucleus, cytoplasm, cell surface and in the extracellular environment. Because of its unique structure, galectin-3 can
oligomerize forming lattice upon binding to multivalent oligossacharides and influence several pathologic events
such as tumorigenesis, invasion and metastasis.
Methods: In our study, balb/c Lgals3+/+ and Lgals3−/− female mice were inoculated in the fourth mammary fat
pad with 4T1 breast cancer cell line. The primary tumor, inguinal lymph nodes and iliac bone marrow were
evaluated 15, 21 and 28 days post-injection. The primary tumor growth was evaluated by measuring the external
diameter, internal growth by ultrasound and weight of the excised tumor. The presence of cancer cells in the
draining lymph nodes and iliac crest bone marrow were performed by immunohistochemistry, PCR and clonogenic
metastatic assay.
Results: In this study we demonstrated that the deletion of galectin-3 in the host affected drastically the in vivo
growth rate of 4T1 tumors. The primary tumors in Lgals3−/− mice displayed a higher proliferative rate (p < 0,05),
an increased necrotic area (p < 0,01) and new blood vessels with a wider lumen in comparison with tumors from
Lgals3+/+ mice (P < 0,05). Moreover, we detected a higher number of 4T1-derived metastatic colonies in the lymph
nodes and the bone marrow of Lgals3−/− mice (p < 0,05). Additionally, healthy Lgals3−/− control mice presented
an altered spatial distribution of CXCL12 in the bone marrow, which may explain at least in part the initial
colonization of this organ in Lgals3−/− injected with 4T1 cells.
Conclusions: Taken together, our results demonstrate for the first time that the absence of galectin-3 in the host
microenvironment favors the growth of the primary tumors, the metastatic spread to the inguinal lymph nodes
and bone marrow colonization by metastatic 4T1 tumor cells.
Como referenciar
PEREIRA, JONATHAS X.; AZEREDO, MARIA C.B.; MARTINS, FELIPE S.; CHAMMAS, ROGER; OLIVEIRA, FELIPE L.; SANTOS, SOFIA N.; BERNARDES, EMERSON S.; EL-CHEIKH, MARCIA C. The deficiency of galectin-3 in stromal cells leads to enhanced tumor growth and bone marrow metastasis. BMC Cancer, v. 16, n. 1, 2016. DOI: 10.1186/s12885-016-2679-1. Disponível em: http://repositorio.ipen.br/handle/123456789/26800. Acesso em: 28 Mar 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.