Comparison between canine and molar swine tooth: tissue and stem cell view
Carregando...
Data
2016
Data de publicação:
Autores IPEN
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
Mathews Journal of Veterinary Science
Exportar
Resumo
Dental tissues are an abundant and rich source for easily and continually obtaining of mesenchymal stem cells (MSC),
which are able to differentiate in vitro into several types of tissues, such as fat, cartilage, bone, among others. In swine,
canine teeth display continuous growth, suggesting it could represent a different niche of stem cells. In this study, we
compare dental pulp mesenchymal stem cell (sDPSC) niches from canine and molar teeth in swine. Tooth tissues were
obtained and characterized by histological, microscopy and cellular analyses. Tissues were submitted to immunohistochemistry
analysis and showed expression for mesenchymal stem cells markers, such as CD73, CD90, CD105 and for
pluripotent markers (Oct-4, Nanog and Sox-2). Molar and canine sDPSC were also cultured and characterized according
to MSC properties, such as plastic adherence capability, fibroblast-like morphology and cell surface antigen profile. sDPSC
displayed an exponential growth pattern by MTT assay and increased in-vitro differentiation potential for adipogenic and
osteogenic lineages. Tumorigenic test indicated these cells were unable to generate tumor in nude mice. Thus far, stem
cells derived from canine and molar teeth in swine did not expose significant differences related to cell or plasticity markers
and they indicate to be safe for animal cellular therapy use since they are devoid of tumorigenic disposition.
Como referenciar
DIAS, JOAO L.R.M.; RUSSO, FABIELE B.; BRAGA, PATRICIA C.B.B.; CRUZ, RAPHAEL M. de A.R. da; FERNANDES, ISABELLA R.; CUGOLA, FERNANDA R.; NASCIMENTO, NANCI; MIGLINO, MARIA A.; PIGNATARI, GRACIELA C. Comparison between canine and molar swine tooth: tissue and stem cell view. Mathews Journal of Veterinary Science, v. 1, n. 1, p. 1-6, 2016. Disponível em: http://repositorio.ipen.br/handle/123456789/27143. Acesso em: 19 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.