Calcium phosphate fibers coated with collagen: In vivo evaluation of the effects on bone repair
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2016
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Bio-Medical Materials and Engineering
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The aim of this study was to assess the characteristics of the CaP/Col composites, in powder and fiber form, via
scanning electron microscopy (SEM), pH and calcium release evaluation after immersion in SBF and to evaluate the performance
of these materials on the bone repair process in a tibial bone defect model. For this, four different formulations (CaP
powder – CaPp, CaP powder with collagen – CaPp/Col, CaP fibers – CaPf and CaP fibers with collagen – CaPf/Col) were
developed. SEM images indicated that both material forms were successfully coated with collagen and that CaPp and CaPf
presented HCA precursor crystals on their surface. Although presenting different forms, FTIR analysis indicated that CaPp and
CaPf maintained the characteristic peaks for this class of material. Additionally, the calcium assay study demonstrated a higher
Ca uptake for CaPp compared to CaPf for up to 5 days. Furthermore, pH measurements revealed that the collagen coating
prevented the acidification of the medium, leading to higher pH values for CaPp/Col and CaPf/Col. The histological analysis
showed that CaPf/Col demonstrated a higher amount of newly formed bone in the region of the defect and a reduced presence
of material. In summary, the results indicated that the fibrous CaP enriched with the organic part (collagen) glassy scaffold
presented good degradability and bone-forming properties and also supported Runx2 and RANKL expression. These results
show that the present CaP/Col fibrous composite may be used as a bone graft for inducing bone repair.
Como referenciar
UENO, FABIO R.; KIDO, HUELITON W.; GRANITO, RENATA N.; GABBAI-ARMELIN, PAULO R.; MAGRI, ANGELA M.P.; FERNANDES, KELLY R.; SILVA, ANTONIO C. da; BRAGA, FRANCISCO J.C.; RENNO, ANA C.M. Calcium phosphate fibers coated with collagen: In vivo evaluation of the effects on bone repair. Bio-Medical Materials and Engineering, v. 27, n. 2-3, p. 259-273, 2016. DOI: 10.3233/BME-161581. Disponível em: http://repositorio.ipen.br/handle/123456789/27181. Acesso em: 23 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.