Application of photodynamic therapy with led and methylene blue in streptococcus mutans in the presence of glucose- in vitro study
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2017
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Lasers in Surgery and Medicine
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Background: Streptococcus mutans is a microorganism
associated with tooth decay; many genes that express adhesion,
biofilm formation, extracellular polysaccharide, carbohydrate
uptake and acid tolerance regulate its cariogenic properties. S.
mutans inhabits a complex biofilm and it produces a large
amount of exopolysaccharides to promote adhesion and enabling
transport pumps. Photodynamic therapy involves the use of a
photosensitizer (PS), which is absorbed by specific cells followed
by irradiation with visible light, resulting in cell death. The aim of this study was to investigate antimicrobial photodynamic
therapy on Streptococcus mutans in the presence of glucose.
Study: Streptococcus mutans was grown in brain heart infusion
(BHI) at 37 8C for 48 h. Inocula were prepared with pure
colonies, which were suspended in phosphate buffered saline
(PBS) with and without 50mM glucose. One-hundred
micromolar methylene blue was used as photosensitizer and the
experiments were performed with groups (control, irradiated
with LED, FS without irradiation, and PDT 30, 60, and 120 s).
Colony form units were counted and statistically analyzed (oneway
ANOVA and Tukey 5%).
Results: The irradiation as well as the photosensitizer in the
dark did not cause cell death. In contrast, in experiments
without glucose, PDT caused cell death proportional the amount
of light used. The more light, the higher the inactivation of S.
mutans and after 2 min a reduction of 7 orders of magnitude
(100%) was observed. In experiments with glucose cell death
was observed even increasing the radiant exposure.
Conclusion: We concluded that PDT is a viable solution for
inactivation of S. mutans in suspension, and that the presence
of glucose activates efflux pumps in the bacterial cell wall, and
it drastically reduces the effect of PDT.
Como referenciar
LEAL, CINTIA; ALVARENGA, LETICIA; SILVA, TAMIRES; BUSSADORI, SANDRA; KATO, ILKA; RIBEIRO, MARTHA; PRATES, RENATO. Application of photodynamic therapy with led and methylene blue in streptococcus mutans in the presence of glucose- in vitro study. Lasers in Surgery and Medicine, v. 49, n. S28, p. 47-47, 2017. DOI: 10.1002/lsm.22650. Disponível em: http://repositorio.ipen.br/handle/123456789/27823. Acesso em: 24 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.