Knockdown of NF-κB1 by shRNA inhibits the growth of renal cell carcinoma in vitro and in vivo
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2018
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Oncology Research
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Renal cell carcinoma (RCC) accounts for approximately 2-3% of human malignancies
and is the most aggressive among urologic tumors. Biological heterogeneity, drug
resistance and chemotherapy side effects are the biggest obstacles to the effective
treatment of RCC. The NF-кB transcription factor is one of several molecules identified
to be responsible for the aggressive phenotype of this tumor. In the past decade, several
studies have demonstrated the activation of NF-kB in RCC, and many implicated NF-
κB1 (p50) as an important molecule in tumor progression and metastasis. In the present
study, a lentivirus was used to deliver shRNA targeting NF-κB1 into mouse renal cell
carcinoma (Renca) cells. It was determined that the knockdown of the NF-κB1 gene led
to a reduction in cell proliferation and late apoptosis/necrosis in vitro. Flow cytometry
analysis demonstrated G2/M arrest in the cells. In addition, immunoblotting analysis
revealed a significant increase in cyclin B1 and Bax. In vivo experiments showed that
Renca-shRNA-NF-кB1 cells have significantly diminished tumorigenicity. Moreover,
immunohistochemical analysis revealed an increase in necrotic areas of Renca-shRNANF-
кB1 tumors. Thus, this study indicates that downregulation of NF-кB1 can suppress
RCC tumorigenesis by inducing late apoptosis/necrosis. Therefore, NF-кB1 may be a
potential therapeutic target for RCC.
Como referenciar
IKEGAMI, AMANDA; TEIXEIRA, LUIZ F.S.; BRAGA, MARINA S.; DIAS, MATHEUS H. dos S.; LOPES, EDUARDO C.; BELLINI, MARIA H. Knockdown of NF-κB1 by shRNA inhibits the growth of renal cell carcinoma in vitro and in vivo. Oncology Research, v. 26, n. 5, p. 743-751, 2018. DOI: 10.3727/096504017X15120379906339. Disponível em: http://repositorio.ipen.br/handle/123456789/28516. Acesso em: 18 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.