Treatment of a burn animal model with functionalized tridimensional electrospun biomaterials
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2017
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Journal of Biomaterials Applications
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Laminin-functionalized poly-D,L-lactic acid scaffolds were produced. Following this, mesenchymal stem cells and keratinocytes
were seeded on biomaterials for the in vivo experiments, where the biomaterials with or without cells were
implanted. The analysis is comprised of the visual aspect and mean size of the lesion plus the histology and gene
expression. The results showed that the cells occupied all the structure of the scaffolds in all the groups. After nine
days of in vivo experiments, the defect size did not show statistical difference among the groups, although the groups
with the poly-D,L-lactic acid/Lam biomaterial had the lowest lesion size and presented the best visual aspect of the
wound. Gene expression analysis showed considerable increase of tumor growth factor beta 1 expression, increased
vascular endothelial growth factor and balance of the BAX/Bcl-2 ratio when compared to the lesion group. Histological
analysis showed well-formed tissue in the groups where the biomaterials and biomaterials plus cells were used. In some
animals, in which biomaterials and cells were used, the epidermis was formed throughout the length of the wound. In
conclusion, these biomaterials were found to be capable of providing support for the growth of cells and stimulated the
healing of the skin, which was improved by the use of cells.
Como referenciar
STEFFENS, DANIELA; MATHOR, MONICA B.; SOSTER, PAULA R. da L.; VERGANI, GUSTAVO; LUCO, DAYANE P.; PRANKE, PATRICIA. Treatment of a burn animal model with functionalized tridimensional electrospun biomaterials. Journal of Biomaterials Applications, v. 32, n. 5, p. 663-676, 2017. DOI: 10.1177/0885328217735933. Disponível em: http://repositorio.ipen.br/handle/123456789/28535. Acesso em: 20 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.