Periodontal treatment combined to antimicrobial photodynamic therapy
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2017
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Lasers in Surgery and Medicine
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Background: Antimicrobial photodynamic therapy (APDT) has
been used as an adjuvant treatment for periodontitis. It
combines a photosensitizer (PS) with a light source to induce
reactive oxygen species (ROS) and kill microbial cells. PpNetNI
is a protoporphyrin derivate, and it has a chemical binding site
at biofilm and great affinity to microbial cells. The aim of this
study was to investigate the effects of APDT as an adjuvant
treatment for periodontitis.
Study: Ten healthy male rats Wistar (Rattus norvegicus) were
used in this study (Approved by UNINOVE Ethical committee
AN0029/2015). Periodontitis was induced by placing a cotton
ligature around the first mandibular molar in a subgingival
position. The contralateral mandibular first molar received
neither a ligature nor any treatment, and was used as a
control. After 7 days, the ligature was removed and all
animals received scaling and root planning (SRP) and were
divided according to the following treatments: SRP group
(received SRP and irrigation with PpNetNI, 10 mM) and PDT
group (PpNetNI 10 mM followed by LED irradiation). PDT was
performed with a LED (630 nm) with an output power of
400mW (fluence-rate 200mW/cm2; fluence 18 J/cm2). Rats
were euthanized at 7 days postoperatively. The bone loss was
measured by Optical Coherence Tomography (OCT,
THORLABS LTD., Newton, US). Data were analyzed
statistically (Mann-Whitney test, p<0.05). in vestibular region
of the first molar
Results: The animals treated by APDT showed a bone gain of
approximately 30% compared to the SRP group following 7 days
from the treatment. OCT was able to detect bone loss in the
samples and it was nondestructive method for this experimental
model.
Conclusion: In conclusion, within the parameters used in this
study, APDT was an effective alternative to held periodontal
health after treatment, and it was able to regenerate supportive
periodontal tissue.
Como referenciar
GODOY-MIRANDA, BIANCA; BELINELLO-SOUZA, ESTEFANI; ALVARENGA, LETICIA; LEAL, CINTIA; LEITE, CAROLINA; LIMA, TAIRINE; PREVIATI-OLIVEIRA, JHOSEPHER; FREITAS, ANDERSON; PRETTO, LUCAS de; FERNANDES, ADJACI; PRATES, RENATO. Periodontal treatment combined to antimicrobial photodynamic therapy: experimental model. Lasers in Surgery and Medicine, v. 49, n. S28, p. 42-43, 2017. DOI: 10.1002/lsm.22650. Disponível em: http://repositorio.ipen.br/handle/123456789/28802. Acesso em: 18 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.