Vacuumed collagen-impregnated bioglass scaffolds
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2019
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Journal of Biomedical Materials Research Part B: applied biomaterials
Resumo
This study evaluated physical–chemical characteristics
of a vacuumed collagen-impregnated bioglass (BG) scaffolds
and bone marrow stromal cells (BMSCs) behavior on those composites.
scanning electron microscope and energy dispersive xray
spectroscope demonstrated collagen (Col) was successfully
introduced into BG. Vacuum impregnation system has showed
efficiency for Col impregnation in BG scaffolds (approximately
20 wt %). Furthermore, mass weight decreasing and more stabilized
pH were observed over time for BG/Col upon incubation in
phosphate buffered saline compared to plain BG under same
conditions. Calcium evaluation (Ca assay) demonstrated higher
calcium uptake for BG/Col samples compared to BG. In addition,
BG samples presented hydroxyapatite crystals formation on its
surface after 14 days in simulated body fluid solution, and signs
of initial degradation were observed for BG and BG/Col after 21
days. Fourier transform infrared spectroscopy spectra for both
groups indicated peaks for hydroxyapatite formation. Finally, a
significant increase of BMSCs viability for both composites was
observed compared to control group, but no increase of osteogenic
differentiation-related gene expressions were found. In
summary, BG/Col scaffolds have improved degradation, pH equilibrium
and Ca mineralization over time, accompanied by
hydroxyapatite formation. Moreover, both BG and BG/Col scaffolds
were biocompatible and noncytotoxic, promoting a higher
cell viability compared to control. Future investigations should
focus on additional molecular and in vivo studies in order to
evaluate biomaterial performance for bone tissue engineering
applications.
Como referenciar
KIDO, HUELITON W.; GABBAI-ARMELIN, PAULO R.; AVANZI, INGRID R.; SILVA, ANTONIO C. da; FERNANDES, KELLY R.; FORTULAN, CARLOS A.; RENNO, ANA C.M. Vacuumed collagen-impregnated bioglass scaffolds: characterization and influence on proliferation and differentiation of bone marrow stromal cells. Journal of Biomedical Materials Research Part B: applied biomaterials, v. 107, n. 2, p. 211-222, 2019. DOI: 10.1002/jbm.b.34112. Disponível em: http://repositorio.ipen.br/handle/123456789/28958. Acesso em: 28 Mar 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.