Low level light therapy on breast tumor. In vitro and in vivo studies
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2018
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CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOFISICA, 43.
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Resumo
Low Level Light Therapy (LLLT) has been gaining
prominence in health areas due to search for less
invasive and cost-effective treatments. However, its
use is not indicated to treat patients with cancer due
to a few studies in vivo. The aim of this work was to
evaluate the effects of LLLT on breast tumor 4T1 cells
in vitro and in a murine model. For the in vitro assay,
4T1 cells were submitted to a single session of LLLT
with red light (660 ± 20 nm) delivering energies of
1.2 J and 6 J. The cell viability was measured by MTT
assay. For in vivo study, nine BALB/c female mice
(6 weeks old) received 4T1 cells transfected with
luciferase (4T1-Luc) into the mammary fat pad. After
two weeks, the animals were divided into control (n=3)
and test groups were submitted to LLLT with same
parameters described above. The tumor progression
was monitored by a caliper and bioluminescence. The
anti-fatigue effect of LLLT was assessed by a forced
swimming test. Our results showed that LLLT was not
able to increase cell viability regardless the energy
used. In vivo, the lower energy promoted an increase
of the tumor volume compared to control group. On the
other hand, the higher energy was able to arrest tumor
progression as well as increase the swimming time.
Taken together, our results suggest that LLLT triggers
opposite effects on breast tumor depending on the type
of assay.
Como referenciar
PEREIRA, SAULO; CONTATORI, CAROLINA; RIBEIRO, MARTHA S. Low level light therapy on breast tumor. In vitro and in vivo studies. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOFISICA, 43., 27-30 de setembro, 2018, Santos, SP. Resumo... Rio de Janeiro: Sociedade Brasileira de Biofísica, 2018. p. 62-62. Disponível em: http://repositorio.ipen.br/handle/123456789/29722. Acesso em: 25 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.