Tryptophan silver nanoparticles synthesized by photoreduction method
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2019
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International Journal of Tryptophan Research
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The high rates of antibiotics use in hospitals have resulted in a condition where multidrug-resistant pathogens have become a severe
threat to the human health worldwide. Therefore, there is an increasing necessity to identify new antimicrobial agents that can inhibit the multidrugresistant
bacteria and biofilm formation. In this study, antibacterial and anti-biofilm activities of tryptophan silver nanoparticles (TrpAgNP) were
investigated. The TrpAgNPs were synthesized by photoreduction method, and the influence of irradiation time and concentration of reagents
were analyzed. The nanoparticles were characterized by transmission electron microscopy, Zeta Potential and (UV)-absorption spectra. The
antibacterial activity of TrpAgNPs was tested for antibiotic-resistant and susceptible pathogens, Staphylococcus aureus, Staphylococcus
epidermidis, Escherichia coli, Citrobacter freundii, Klebsiella pneumoniae, Salmonella typhimurium, and Pseudomonas aeruginosa, evaluating
the influence of photoreduction parameters in bactericidal effect. The results have shown that TrpAgNPs solutions with lower tryptophan/silver
nitrate (AgNO3) ratio and higher AgNO3 concentration have higher bactericidal action against bacteria with inhibition of ~100% in almost all
studied bacterial strains. The antimicrobial activity of TrpAgNPs within biofilms generated under static conditions of antibiotic-resistant and
susceptible strains of S. aureus, S. epidermidis, E. coli, K. pneumoniae, C. freundii, and P. aeruginosa was also investigated. The results showed
that TrpAgNPs have an inhibitory effect against biofilm formation, exceeding 50% in the case of Gram-negative bacteria (E. coli, K. pneumoniae,
C. freundii, and P. aeruginosa—54.8% to 98.8%). For Gram-positive species, an inhibition of biofilm formation of 68.7% to 72.2 % was observed
for S. aureus and 20.0% to 40.2% for S. epidermidis.
Como referenciar
COURROL, DANIELLA dos S.; LOPES, CARLA R.B.; PEREIRA, CAMILA B.P.; FRANZOLIN, MARCIA R.; SILVA, FLAVIA R. de O.; COURROL, LILIA C. Tryptophan silver nanoparticles synthesized by photoreduction method: characterization and determination of bactericidal and anti-biofilm activities on resistant and susceptible bacteria. International Journal of Tryptophan Research, v. 12, p. 1-8, 2019. DOI: 10.1177/1178646919831677. Disponível em: http://repositorio.ipen.br/handle/123456789/30045. Acesso em: 24 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.