Intramuscular growth hormone plasmid DNA eletrotransfer effects on bone quality in a murine model of osteogenesis imperfecta
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2019
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Journal of Cell Science & Therapy
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Osteogenesis imperfecta (OI) is a congenital dysplasia of connective tissue characterized mainly by fragility and low bone density. The type I OI, a mild form of the disease, is associated with the quantitative decrease of type
I collagen in the extracellular matrix, a characteristic also observed in the oim mice used in pre-clinical OI research.
Previous studies have shown the efficiency of recombinant human growth hormone (GH) treatment for OI, both in
animal and human models, reducing bone fragility, increasing bone density and stimulating α 1 and α 2 procollagen
synthesis. This work aimed at using plasmid containing the murine GH (mGH) gene to treat oim heterozygous
mice. Serological quantification of mGH by ELISA was carried out to evaluate the plasmid expression, bone density
by DEXA and three-point flexion test were performed on the femurs to assess bone quality. In a short-term (3-day)
trial, mGH levels of treated animals were 20.6±6.6 ng/mL, versus 3.3±2.2 ng/mL for those receiving saline and
2.3±1.3 ng/mL for the untreated wild-type group. A 90-day assay with plasmid applications at 0, 30 and 60 days
was performed in parallel. The results of bone density showed greater effectiveness of the hormone during the first
month of treatment, with a 34% increase in comparison with the saline group, whereas in the second month there
was no significant statistical difference (p>0.05). The three-point bending test and the final analysis of bone density
are currently being carried out.
Como referenciar
JESUS, GUSTAVO P.P. de; GOMES, ALISSANDRA de M.; ZACARIAS, ENIO A.; YOSIDAKI, VANESSA de L.; BARTOLINI, PAOLO; PERONI, CIBELE N. Intramuscular growth hormone plasmid DNA eletrotransfer effects on bone quality in a murine model of osteogenesis imperfecta. Journal of Cell Science & Therapy, v. 10, p. 36-36, 2019. DOI: 10.4172/2157-7013-C2-053. Disponível em: http://repositorio.ipen.br/handle/123456789/30303. Acesso em: 23 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.