The effects of radiation and experimental conditions over papain nanoparticle formation
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2020
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Radiation Physics and Chemistry
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Papain is a natural enzyme extracted from the fruit of Carica papaya Linnaeus, successfully applied in the
pharmaceutical area as a drug carrier and debridement agent for wounds. In recent studies papain nanoparticles
were synthesized and crosslinked with the use of ionizing radiation in the search for biopharmaceutical advantages
as well as the development of bioactive nanocarriers. This study addresses the effects of buffer molarity
and irradiation conditions on papain nanoparticles formation. Nanoparticles were synthesized on ice bath using
ethanol (20%, v/v) as a cosolvent and crosslinked by gamma radiation using a 60Co source. Experimental
variables included the synthesis in deionized water and in 1, 10, 25 and 50mM phosphate buffer, under different
temperatures of −20 °C, 0 °C and 20 °C before and throughout the irradiation period, and using radiation dose
rates of 0.8, 2, 5 and 10 kGy h−1 to reach the dose of 10 kGy. Proteolytic activity was quantified using Nα-
benzoyl-DL-arginine-p-nitroanilide hydrochloride. Nanoparticle size and crosslinking by means of bityrosine
were evaluated by dynamic light scattering and fluorescence measurements, respectively. Buffer molarity and
radiation dose rate were identified to influence bityrosine formation and proteolytic activity without impacting
nanoparticle size. Variations in temperature impacted bityrosine formation exclusively. Optimized conditions for
papain nanoparticle synthesis were achieved using 50mM phosphate buffer at the dose rate of 5 kGy h−1 and
temperature of 0 °C throughout the process.
Como referenciar
FAZOLIN, GABRIELA N.; VARCA, GUSTAVO H.C.; KADLUBOWSKI, SLAWOMIR; SOWINSKI, SEBASTIAN; LUGAO, ADEMAR B. The effects of radiation and experimental conditions over papain nanoparticle formation: towards a new generation synthesis. Radiation Physics and Chemistry, v. 169, p. 1-8, 2020. DOI: 10.1016/j.radphyschem.2018.08.033. Disponível em: http://repositorio.ipen.br/handle/123456789/31072. Acesso em: 23 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.