Preclinical investigation of methylene blue-mediated antimicrobial photodynamic therapy on Leishmania parasites using real-time bioluminescence
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2020
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Photochemistry and Photobiology
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Cutaneous leishmaniasis (CL) is a neglected disease that promotes
destructive lesions. Difficulties in treatment are related
to accessibility of drugs, resistance and toxicity. Antimicrobial
photodynamic therapy (APDT) has been emerging as a
promising treatment for CL. In this work, we evaluated
methylene blue (MB)-mediated APDT (MB-APDT) on Leishmania
amazonensis in vitro and in vivo by bioluminescence
technique. In vitro, MB-APDT was performed using a red
LED (k = 660 11 nm, 100 mW cm 2) and MB (100 μM) at
different light doses. In vivo, mice were infected and 4 weeks
later, randomly divided into three groups: control, APDT 1
(single session) and APDT 2 (two sessions of MB-APDT). MB
was used at 100 μM and energy dose was established at
150 J cm 2. Parasite burden, lesion size and pain were evaluated
weekly for 4 weeks. In vitro, lethal dose for 90% parasite
inactivation was achieved at 48.8 J cm 2. In vivo,
although APDT 1 and APDT 2 groups have showed similar
parasite burden after 4 weeks, two sessions were clinically
better, especially considering the inflammatory process associated
to CL. Our findings reinforce MB-APDT as a costeffective
treatment to combat CL.
Como referenciar
CABRAL, FERNANDA V.; SABINO, CAETANO P.; DIMMER, JESICA A.; SAUTER, ISMAEL P.; CORTEZ, MAURO J.; RIBEIRO, MARTHA S. Preclinical investigation of methylene blue-mediated antimicrobial photodynamic therapy on Leishmania parasites using real-time bioluminescence. Photochemistry and Photobiology, v. 96, n. 3, p. 604-610, 2020. DOI: 10.1111/php.13188. Disponível em: http://repositorio.ipen.br/handle/123456789/31330. Acesso em: 18 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.