Photodynamic activity on biofilm in endotracheal tubes of patients admitted to an intensive care unit
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2020
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Photochemistry and Photobiology
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Resumo
Ventilator-associated pneumonia (VAP) is an infection that
arises after endotracheal intubation affecting patients under
intensive care. The presence of the endotracheal tube
(ETT) is a risk factor since it is colonized by multispecies
biofilm. Antimicrobial photodynamic therapy (aPDT) could
be a strategy to decontaminate ETTs. We verify if methylene
blue (MB) associated with external illumination of the
ETT could be an alternative to destroy biofilm. We performed
an in vitro and ex vivo study. In vitro study was
performed with P. aeruginosa biofilm grew over ETT for
7 days. After treatment, the surviving cells were cultured
for 3 days and the biofilm was analyzed by crystal violet
absorbance. Ex vivo study employed ETT obtained from
extubated patients. aPDT was performed with MB (100 μM)
and red LED (k = 640±20 nm). We quantified the biofilm
thickness and used scanning electron microscopy and fluorescence
technique to verify morphological and functional
changes after aPDT. Our results showed that bacteria
remain susceptible to aPDT after sequential treatments. We
also attested that aPDT can reduce biofilm thickness,
disrupt biofilm attachment from ETT surface and kill
microbial cells. These data suggest that aPDT should
be investigated to decrease VAP incidence via ETT
decontamination.
Como referenciar
SOARES, ROSANE B.; COSTA, DENIS H.; MIYAKAWA, WALTER; DELGADO, MARIA G.T.; GARCEZ, AGUINALDO S.; YOSHIMURA, TANIA M.; RIBEIRO, MARTHA S.; NUNEZ, SILVIA C. Photodynamic activity on biofilm in endotracheal tubes of patients admitted to an intensive care unit. Photochemistry and Photobiology, v. 96, n. 3, p. 618-624, 2020. SI. DOI: 10.1111/php.13239. Disponível em: http://repositorio.ipen.br/handle/123456789/31414. Acesso em: 17 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.