Paratrygon aiereba irradiated anti-mucus serum reduce edematogenic activity induced in experimental model
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2020
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Toxicon
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Accidents by freshwater stingrays are common in northern Brazil, there is no specific therapy for high morbidity
and local tissue destruction. The irradiation of venoms and toxins by ionizing radiation has been used to produce
appropriate immunogens for the production of antisera. We planned to study the efficacy of stinging mucus
irradiation in the production of antisera, with serum neutralization assays of edematogenic activity and quantification
of cytokines performed in animal models of immunization with native and irradiated mucus of Paratrygon
aiereba, a large freshwater stingray. Antiserum potency and its cross-reactivity with mucus from other
freshwater stingrays were detected by ELISA. Immunization models demonstrated the ability to stimulate a
strong humoral response with elevated levels of serum IgG detectable by ELISA, and both native and irradiated
mucus were immunogenic and capable of recognizing mucus proteins from other freshwater neotropical stingrays.
Mucus P. aiereba causes cellular and humoral adaptive immune responses in cells of immunized mice
producing antibodies and cytokines such as TNF-α, IL-6 and IL-17. Rabbit antisera immunized with mucus from
P. aiereba irradiated at 2 kGy showed a significant reduction of mucus-induced edematogenic activity in mice.
Our data suggest that the use of antisera against freshwater stingray mucus show the possibility of specific
therapy for these accidents.
Como referenciar
THOMAZI, GABRIELA O.C.; COSTA, ANDREA da; RODRIGUES, JAQUELINE P.; ALVES, GLAUCIE J.; PREZOTTO NETO, JOSE P.; TURIBIO, THOMPSON de O.; ROCHA, ANDRE M.; AIRES, RAQUEL da S.; SEIBERT, CARLA S.; SPENCER, PATRICK J.; GALISTEO JUNIOR, ANDRES J.; ANDRADE JUNIOR, HEITOR F. de; NASCIMENTO, NANCI do. Paratrygon aiereba irradiated anti-mucus serum reduce edematogenic activity induced in experimental model. Toxicon, v. 178, p. 13-19, 2020. DOI: 10.1016/j.toxicon.2020.02.012. Disponível em: http://repositorio.ipen.br/handle/123456789/31450. Acesso em: 20 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.