Er,Cr:YSGG laser irradiation associated to fluoride for in situ model using gamma sterilized dentin and enamel
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2019
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Photobiomodulation, Photomedicine, and Laser Surgery
Resumo
The in situ intraoral model uses human dental enamel samples
(HDE) in order to analyse the de-remineralization processes using
the buccal environment without interfering into the patients’
natural dentition. The main ethical concern from this model is the
biosafety. Gamma radiation is a very efficient sterilization method
that is not expected to alter the mineral content of the hard tissues,
avoiding biases in the results. Thus 40 HDE samples were irradiated
through a source of 60Co multipurpose irradiator aiming
complete sterilization (25 KGy/h) with the purpose of accumulating
the native plaque on them at an in situ study. An Er,Cr:YSGG laser
was used alone and in combination with the topical applications of:
1-dentifrice (1,100 lg F-/g) or 2-APF (12,300 lg F-/g). Morphological
analyses were performed by scanning electron microscopy
(SEM), determination of alkali-soluble fluoride concentration by
specific ion electrode and microhardness determination. Then, the
15 volunteers used palatal devices containing previously treated
HDE samples and remained using F dentifrice. The FTIR findings
established that gamma radiation could be used aiming HDE
sterilization. The Knoop hardness number was within the range
of that of natural dentin of human origin. X-ray fluorescence
shows that irradiated dentin has great similarity with natural
dentin from the point of view of chemical composition. SEM
analyses showed that there was no thermal damage or interprismatic
morphological changes in the hydroxyapatite structure of
human dental dentin outside the buccal environment when using
doses of gamma irradiation up to 25 kGy.
Como referenciar
ZAMATARO, CLAUDIA B.; RABELO, THAIS F.; ZANINI, NATHALIA; JUVINO, AMANDA C.; KUCHAR, NIELSEN G.; CASTRO, PEDRO A.L.; ANA, PATRICIA A. da; ZEZELL, DENISE M. Er,Cr:YSGG laser irradiation associated to fluoride for in situ model using gamma sterilized dentin and enamel. Photobiomodulation, Photomedicine, and Laser Surgery, v. 37, n. 10, p. A13-A13, 2019. DOI: 10.1089/photob.2019.29013.abstracts. Disponível em: http://repositorio.ipen.br/handle/123456789/32221. Acesso em: 20 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.