Assessment of burn wounds status using mid-infrared spectroscopy
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2019
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SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE
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Resumo
Burns are one of the major causes of morbidity and the most costly traumatic
injuries worldwide. Better understanding of the molecular mechanisms associated with
wound healing might provide improved clinical strategies to speed up the tissue repair
process and reduce the global impact of burns on public health services. The traditional
techniques used to assess the biochemical events related to wound repair are laborious,
time-consuming and require multiple staining. Thus, the present study aims to evaluate
the feasibility of Fourier transform infrared (FTIR) spectroscopy in order to monitor the
progress and healing status of burn wounds. Third-degree burn injuries were induced on
Wistar rats by water vapor exposure. Afterwards, biopsies specimen was extracted for
further histopathological examination and spectroscopic evaluation at 4 time-points (3,
7, 14 and 21 days). Raw spectral data were offset-corrected and normalized by amide I
band area. The second derivatives were compared by the Principal Component Analysis (PCA). On days 3 and 7, when compared to healthy group, biomolecules bands were most
prominent. However, on days 14 and 21, these molecular bands decreased. Therefore,
our pairwise comparison revealed that metabolic activity induced by thermal injury
decreases as the healing process progresses. Our findings show that FTIR spectroscopy
can monitor the biochemical development induced by burn injury and detect the status of
wound repair.
Como referenciar
CASTRO, PEDRO; LIMA, CASSIO; ZORN, TELMA; ZEZELL, DENISE M. Assessment of burn wounds status using mid-infrared spectroscopy. In: SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE, October 7-9, 2019, São Paulo, SP. Abstract... Piscataway, NJ, USA: IEEE, 2019. p. 20-21. Disponível em: http://repositorio.ipen.br/handle/123456789/32225. Acesso em: 20 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.