A comparison between two populations of mesenchymal stem cells that can improve the osteogenesis imperfecta mouse phenotype
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2021
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CONGRESS OF THE BRAZILIAN SOCIETY FOR CELL BIOLOGY, 20th
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Resumo
Osteogenesis imperfecta (OI) is an inherited disease characterized by fragility,
deformity and low bone density, besides other clinical manifestations. Type-I OI is
the mildest and most common form of the disease, caused by a mutation in the
COL1A1 gene and resulting in half normal-collagen production. Our purpose was to
compare mesenchymal stem cells from bone marrow (BM-MSCs) with those from
adipose tissue (AD-MSCs), both used for improving heterozygous oim mice
phenotype, similar to human type-I OI. Mice were irradiated and cells injected into
femoral condyles, bone mineral density (BMD) and femoral length were measured
by X-ray, at the beginning and end of the assay. Femurs and quadriceps allowed
bone fragility evaluation by biomechanical test and collagen Col1a1 and Col1a2
quantification via ELISA. BMD showed no significant difference between the groups,
while femur length variation was higher in the BM-MSCs group, compared with the
control (P=0.0301). Fragility was improved with AD-MSCs when flexion extension
to fracture (P=0.0028) and time to fracture (P=0.0032) were evaluated. There was
no significant difference in the maximum load supported by femurs until fracture.
An increase in Col1a1 concentration with BM-MSCs in comparison with AD-MSCs
(P=0.0281) was obtained. Although no significant difference in Col1a2
concentration was observed between the groups, a higher expression level was
obtained with AD-MSCs. We can thus conclude that AD-MSCs were more efficient
than BM-MSCs for improving bone quality in type-I OI.
Como referenciar
GOMES, ALISSANDRA de M.; JESUS, GUSTAVO P.P. de; ZACARIAS, ENIO A.; YOSIDAKI, VANESSA L.; LIMA, DARLE B. de; COSTA, RAFAEL S.; RANGEL, ERIKA B.; SOUSA, POLIANA E.S.; SILVA, CHRISTIAN S.; BARTOLINI, PAOLO; PERONI, CIBELE N. A comparison between two populations of mesenchymal stem cells that can improve the osteogenesis imperfecta mouse phenotype. In: CONGRESS OF THE BRAZILIAN SOCIETY FOR CELL BIOLOGY, 20th, January 27-29, 2021, Online. Abstract... São Paulo, SP: Sociedade Brasileira de Biologia Celular, 2021. p. 249-250. Disponível em: http://repositorio.ipen.br/handle/123456789/32682. Acesso em: 19 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.