A new approach for purification of the catalytic site of the Angiotensin Conversion Enzyme, N domain, mediated by the ELP-Inten system
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2021
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CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th
Resumo
Angiotensin-converting enzyme I, ACE, is a key part of the renin-angiotensin system whose main function is to
regulate blood pressure and balance of salts in the body. ACE1 has two isoforms, somatic, sACE, and testicular,
tACE. sACE possesses two domains, N- C-, with catalytic sites which exhibit 60% sequence identity. These domains
differ in terms of chloride-ion activation profiles, rates of peptide hydrolysis and sensitivities to various inhibitors. N-domain
has specific action in the hydrolyze of Alzheimer’s diseases beta amyloid bodies and angiotensin 1-7, which
active the MAS receptor and triggering anti-thrombotic and anti-inflammatory actions. The objective this work was to
obtain catalytic site Ala361 to Gli468 of the N-domain region, csACEN, isolation without chromatographic and
denaturant chemical process. For that, a new methodology was used in the expression of the csACEN peptide, in
which the peptide was linked to the elastin-like polypeptide, ELP, and Intein, and expressed at 37C. The
characterization of catalytic site was made by SDS-PAGE and dot blotting. The culture temperature at 37C
significantly increased the expression of the ELP/Intein/csACEN fusion protein. This culture was lysed at a low
temperature allowing the fusion protein to become soluble. The precipitation of ELP at high concentrations of
ammonium sulfate were obtained in 0.57 M and 0.8 M. Intein autocleavage occurs at acidic pH and it is important to
pay attention to: pI 6.65 for csACEN and pI 6.87 for ELPcsACEN, which are very low. The best autocleavage
efficiency was with MES and TriHCl buffers, pH 6.3 and 6.8, respectively, in which pure csACEn peptide was
obtained. The strategy used to obtain the Ala361 to Gli468 catalytic site in soluble and pure form was obtained with
success and the protocol for obtaining similar peptides was established.
Como referenciar
SANTOS, CAROLINA M. dos; SAMPAIO, SUELEN de B.; SANTANA, FAGNER; LEITE, RODRIGO C.; PRATA, BEATRIZ A.; AFFONSO, REGINA. A new approach for purification of the catalytic site of the Angiotensin Conversion Enzyme, N domain, mediated by the ELP-Inten system. In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 143-143. Disponível em: http://repositorio.ipen.br/handle/123456789/32867. Acesso em: 25 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.