Reconstitution of Leishmania plasma membrane to understand the photodynamic effect
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2021
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CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th
Resumo
Leishmaniasis is an important neglected disease. Photodynamic therapy (PDT) has been used to fight cutaneous
leishmaniasis showing good results. However, PDT mechanisms in Leishmania parasites are not yet completely
clarified. In this work, our objective was to develop a protocol to produce giant plasma membrane vesicles (GPMVs)
from Leishmania amazonensis promastigotes to understand the mechanisms of action of methylene blue (MB)-
mediated PDT on the cell membrane of parasites. For membrane extraction, several techniques were tested. The
osmotic shock was the technique that presented the best yield and effectiveness. Phosphate and protein
measurements were performed to confirm membrane extraction. For the growth of GPMVs, the best technique was
electroforming using different frequencies and voltages in 4 cycles. Reconstituted GPMVs were observed by phasecontrast
light microscopy. Subsequently, PDT was applied to GPMVs dispersed in an aqueous solution containing
50 μM MB and we verified the changes in permeability before and after exposure to light. The same process was
applied to giant unilamellar vesicles (GUVs) with lipid compositions similar to the parasite membrane. The
electroforming technique with the protocol developed in this work made it possible to obtain GPMVs from a
promastigote membrane isolate of L. amazonensis. The membrane isolation technique was effective to extract the
parasite's membrane while preserving lipids and proteins. In GUVs we observe an increase in the area during PDT
in different compositions and loss of contrast. The GPMVs showed a loss of contrast as well as the GUVs but did not
show an increase in area. This factor could be explained by the high degree of complexity of the membrane, which
contains membrane proteins in addition to containing lipids.
Como referenciar
SOUZA, MARESSA D.F. de; ITRI, ROSANGELA; RIBEIRO, MARTHA S. Reconstitution of Leishmania plasma membrane to understand the photodynamic effect. In: CONGRESS OF THE INTERNATIONAL UNION FOR PURE APPLIED BIOPHYSICS, 20th; ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, 50th; CONGRESS OF BRAZILIAN BIOPHYSICS SOCIETY, 45th; BRAZILIAN SOCIETY ON NUCLEAR BIOSCIENCES CONGRESS, 13th, October 4-8, 2021, São Paulo, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq), 2021. p. 255-255. Disponível em: http://repositorio.ipen.br/handle/123456789/32871. Acesso em: 28 Mar 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.