Use of SNOM for analysis of biomarker molecules when excited by Raman
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2021
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WORKSHOP ON LIDAR MEASUREMENTS IN LATIN AMERICA, 11th
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During the last decade, many studies have made significant progress in understanding the causes
and implications of our environment, with respect to the atmosphere. Bioaerosols are fundamental in the
reproduction of plants and microorganisms (pollen, spores, etc.), which in the atmosphere are transported across
borders and long distances. For this reason, bioaerosols have great importance in genetic diffusion between
habitats and biomas geographic change, contributing as main elements in the development, evolution and
ecosystems dynamics. The consequences related to public health and agriculture are associated by the dispersion
of plants, animals, pathogens and human allergens, showed an increase of allergies and asthma cases during
storms, due to high concentrations of bioaerosols, especially when attributed to fungal spores. The biological
content found in the air of global land surfaces corresponds to a quarter of the total particles found in the air,
consisting mainly of proteins, arising from pollens, fungal spores, bacteria, viruses or fragments of plant or
animal matter. Some proteins act as marker molecules which autofluoresce if excited at a specific wavelength. A
signature of biomarker proteins, existing in the cell wall of fungal spores of medical interest, will be developed
using scanning near-field optical microscopy (SNOM). This new high resolution optical methodology will allow
detailed analysis of biomarker molecules (ergosterol, mannitol and arabitol), as well as their behavior when
excited by Raman. In possession of a molecular standard, a database of these characteristics will be created,
which will provide technical support for the remote Raman sensing of these species in the atmosphere of the
Metropolitan Region of São Paulo (MRSP).
Como referenciar
MACEDO, FERNANDA M.; LANDULFO, EDUARDO. Use of SNOM for analysis of biomarker molecules when excited by Raman. In: WORKSHOP ON LIDAR MEASUREMENTS IN LATIN AMERICA, 11th, October 19-22, 2021, Punta Arenas, Chile. Abstract... Punta Arenas, Chile: Universidad de Magallanes, 2021. p. 27-27. Disponível em: http://repositorio.ipen.br/handle/123456789/33314. Acesso em: 23 Apr 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.