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  • IPEN-DOC 23938

    MUCCILLO, R. ; MUCCILLO, E.N.S. . Electric field assisted sintering of electroceramics and in situ analysis by impedance spectroscopy. Journal of Electroceramics, v. 38, n. 1, p. 24-42, 2017. DOI: 10.1007/s10832-016-0054-x

    Abstract: Since the first report several years ago on pressureless sintering of yttria-stabilized zirconia with several seconds at relatively low temperatures by application of an electric field, an increasing number of scientific reports have been published on sintering ion conducting, semiconducting and insulating polycrystalline electroceramics. The electric field-assisted sintering consists in applying an electric field either during heating up or under isothermal conditions at temperatures well below the ones applied during conventional sintering. Besides the lower temperatures, shorter times are required to achieve full densification without considerable grain growth, evidencing the potential of this technique for obtaining functional electroceramics with improved mechanical and electrical properties at lower costs. Even though some mechanisms have been suggested, the description of the phenomenon at the microscopic level leading to full densification with seconds remains a challenge. We report in situ electrochemical impedance spectroscopy of an ionic conductor (ZrO2: 8 mol% Y2O3), a proton conductor (BaCe0.8Zr0.1Y0.1O3-delta) and a semiconductor (SnO2: 0.5 mol% MnO2) performed during conventional and electric field-assisted sintering experiments. Attention is also directed on the description of the experimental setups and procedures and to the evaluation of microstructural details by scanning electron microscopy. The analysis of the in situ impedance spectroscopy diagrams under heating (before either conventional or electric field-assisted sintering) and under cooling (after) provides evidence of densification with pore elimination and welding of grains. Prospects for future experimental and simulation research work are outlined.

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  • IPEN-DOC 23937

    CONCEICAO, LEANDRO da ; LAY, SABINE; ROBAUT, FLORENCE; RENOU, GILLES; MUCCILLO, E.N.S. ; DJURADO, ELISABETH. Transmission electron microscopy characterization of protective La0.7Sr0.3MnO3−delta coatings prepared by electrostatic spray deposition on ferritic alloy. Functional Materials Letters, v. 10, n. 3, p. 1750012-1 - 1750012-5, 2017. DOI: 10.1142/S1793604717500126

    Abstract: The effect of La0:7Sr0:3MnO3 δ (LSM) coating on SS446 steel on air oxidation at 800○C was investigated by transmission electron microscopy. Dense and crack free thin LSM films were prepared by electrostatic spray deposition. The microstructural characterization was carried out on coated and uncoated interconnectors. A thin chromia scale at the alloy interface along with two spinel phases were observed after long term oxidation in both cases. Specimens exhibit, in addition, an SiO2 layer at the interface with steel due to the high content of Si in the steel. Significant changes in the thickness, morphology and composition occurred in the reaction layer for the LSM coated steel. These effects are explained on the basis of changes in the diffusive fluxes during exposure to the oxidation treatment. The implications of these effects for the degradation mechanism of LSM-based interconnects are discussed.

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  • IPEN-DOC 23936

    OLIVEIRA, ERICA A. ; FAINTUCH, BLUMA L. ; NUNEZ, EUTIMIO G.F. ; MORO, ANA M.; NANDA, PRASANT K.; SMITH, CHARLES J.. Radiotracers for different angiogenesis receptors in a melanoma model. Melanoma Research, v. 22, n. 1, p. 45-53, 2012. DOI: 10.1097/CMR.0b013e32834e6a7e

    Abstract: Early and reliable diagnosis of melanoma, a skin tumor with a poor prognosis, is extremely important. Phage display peptide libraries are a convenient screening resource for identifying bioactive peptides that interact with cancer targets. The aim of this study was to evaluate two technetium-99m tracers for angiogenesis detection in a melanoma model, using cyclic pegylated pentapeptide with RGD and NGR motifs conjugated with the bifunctional chelator mercaptoacetyltriglycine (MAG3). The conjugated peptides (10 ll of a lg/ll solution) were labeled with technetium-99m using a sodium tartrate buffer. Radiochemical evaluation was carried out by instant thin-layer chromatography and confirmed by high-performance liquid chromatography. The partition coefficient was determined and internalization assays were performed in two melanoma cell lines (B16F10 and SKMEL28). Biodistribution evaluation of the tracers was carried out in healthy animals at different time points and also in tumor-bearing mice, 120 min post injection. Blocking studies were also conducted by coinjection of cold peptides. The conjugates displayed a rather similar pharmacokinetic profile. They were radiolabeled with high radiochemical purity (> 97%) and both were hydrophilic with preferential renal excretion. Yet, tumor uptake was higher for human than for murine melanoma cells, especially for [99mTc]-MAG3-PEG8-c(RGDyk) (7.85± 2.34%injected dose/g 120 min post injection). The performance of [99mTc]-MAG3-PEG8-c(RGDyk) was better than the NGR tracer with regard to human melanoma uptake. In this sense, it should be considered for future radiotracer studies of tumor diagnosis. Melanoma Res 22:45–53 c 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.

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  • IPEN-DOC 23935

    SEO, DANIELE ; FAINTUCH, BLUMA L. ; OLIVEIRA, ERICA A. de; FAINTUCH, JOEL. Pancreas and liver uptake of new radiolabeled incretins (GLP-1 and Exendin-4) in models of diet-induced and diet-restricted obesity. Nuclear Medicine and Biology, v. 49, p. 57-64, 2017. DOI: 10.1016/j.nucmedbio.2017.03.002

    Abstract: Introduction: Radiolabeled GLP-1 and its analog Exendin-4, have been employed in diabetes and insulinoma. No protocol in conventional Diet-Induced Obesity (DIO), and Diet-Restricted Obesity (DRO), has been identified. Aiming to assess pancreatic beta cell uptake in DIO and DRO, a protocol was designed. Methods: GLP-1-βAla-HYNIC and HYNIC-βAla-Exendin-4 were labeled with technetium-99m. Four Swiss mouse models were adopted: Controls (C), Alloxan Diabetes Controls (ADC), DIO and DRO. Biodistribution and ex-vivo planar imaging were documented. Results: Radiolabeling yield was in the range of 97% and both agents were hydrophilic. Fasting Blood Glucose (FBG) was 79.2 ± 8.2 mg/dl in C, 590.4 ± 23.3 mg/dl in ADC, 234.3 ± 66.7 mg/dl in DIO, and 96.6 ± 9.3 in DRO (p = 0.010). Biodistribution confirmed predominantly urinary excretion. DIO mice exhibited depressed uptake in liver and pancreas, for both radiomarkers, in the range of ADC. DRO only partially restored such values. 99mTc-HYNIC-βAla-Exendin-4 demonstrated better results than GLP-1-βAla-HYNIC-99mTc. Conclusions: 1) Diet-induced obesity remarkably depressed beta cell uptake; 2) Restriction of obesity failed to normalize uptake, despite robust improvement of FBG; 3) HYNIC-βAla-Exendin-4 was the most useful marker; 4) Further studies are recommended in obesity and dieting, including bariatric surgery.

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  • IPEN-DOC 23934

    FAINTUCH, BLUMA L. ; OLIVEIRA, ERICA A. ; MUNOZ, JULIAN E.; TRAVASSOS, LUIZ R.; TABORDA, CARLOS P.. Radiochemical pharmacokinetic profile of P10 peptide with antifungal properties. Medical Mycology, v. 52, n. 5, p. 1-5, 2014. DOI: 10.1093/mmy/myu024

    Abstract: Paracoccidioidomycosis (PCM) is a chronic granulomatous disease that is caused by the thermally dimorphic fungus Paracoccidioides brasiliensis. It is endemic in some countries of Latin America and can cause a high-burden fungal infection with significant morbidity and mortality. The peptide P10, which demonstrates immune protection against experimental PCM, was radiolabeled with a radioisotope and evaluated in vivo. The radiolabeling was conducted to trace the pharmacokinetics of the molecule in principal organs and tissues. This was achieved with high radiochemical purity. Biodistribution and scintigraphic imaging showed fast blood clearance that was mainly renal; however, hepatobiliar excretion was also, withmarked uptake in cervical lymph nodes. This profile may be useful for the development of a prophylactic drug or vaccine for patients exposed to PCM.

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  • IPEN-DOC 23933

    FAINTUCH, BLUMA L. ; SEO, DANIELE ; OLIVEIRA, ERICA A. de ; TARGINO, ROSELAINE C.; MORO, ANA M.. Evaluation of the influence of the conjugation site of the chelator agent HYNIC to GLP1 antagonist radiotracer for insulinoma diagnosis. Current Radiopharmaceuticals, v. 10, n. 1, p. 65-72, 2017. DOI: 10.2174/1874471010666170126143636

    Abstract: Background and Objective: Radiotracer diagnosis of insulinoma, can be done using somatostatin or glucagon-like peptide 1 (GLP-1). Performance of GLP-1 antagonists tends to be better than of agonists. Methods: We investigated the uptake of the antagonist exendin (9-39), radiolabeled with technetium-99m. Two different sites of the biomolecule were selected for chelator attachment. Results: HYNIC-beta Ala chelator attached to serine (C-terminus) of exendin, was associated with higher tumor uptake than to aspartate (N-terminus). Conclusion: The chelator position in the biomolecule influenced receptor uptake.

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  • IPEN-DOC 23932

    ROSSI, DIEGO C.; MUNOZ, JULIAN E.; CARVALHO, DANIELLE D.; BELMONTE, RODRIGO; FAINTUCH, BLUMA ; BORELLI, PRIMAVERA; MIRANDA, ANTONIO; TABORDA, CARLOS P.; DAFFRE, SIRLEI. Therapeutic use of a cationic antimicrobial peptide from the spider Acanthoscurria gomesiana in the control of experimental candidiasis. BMC Microbiology, v. 12, 2012. DOI: 10.1186/1471-2180-12-28

    Abstract: Background: Antimicrobial peptides are present in animals, plants and microorganisms and play a fundamental role in the innate immune response. Gomesin is a cationic antimicrobial peptide purified from haemocytes of the spider Acanthoscurria gomesiana. It has a broad-spectrum of activity against bacteria, fungi, protozoa and tumour cells. Candida albicans is a commensal yeast that is part of the human microbiota. However, in immunocompromised patients, this fungus may cause skin, mucosal or systemic infections. The typical treatment for this mycosis comprises three major categories of antifungal drugs: polyenes, azoles and echinocandins; however cases of resistance to these drugs are frequently reported. With the emergence of microorganisms that are resistant to conventional antibiotics, the development of alternative treatments for candidiasis is important. In this study, we evaluate the efficacy of gomesin treatment on disseminated and vaginal candidiasis as well as its toxicity and biodistribution. Results: Treatment with gomesin effectively reduced Candida albicans in the kidneys, spleen, liver and vagina of infected mice. The biodistribution of gomesin labelled with technetium-99 m showed that the peptide is captured in the kidneys, spleen and liver. Enhanced production of TNF-a, IFN-g and IL-6 was detected in infected mice treated with gomesin, suggesting an immunomodulatory activity. Moreover, immunosuppressed and C. albicansinfected mice showed an increase in survival after treatment with gomesin and fluconazole. Systemic administration of gomesin was also not toxic to the mice Conclusions: Gomesin proved to be effective against experimental Candida albicans infection. It can be used as an alternative therapy for candidiasis, either alone or in combination with fluconazole. Gomesin’s mechanism is not fully understood, but we hypothesise that the peptide acts through the permeabilisation of the yeast membrane leading to death and/or releasing the yeast antigens that trigger the host immune response against infection. Therefore, data presented in this study reinforces the potential of gomesin as a therapeutic antifungal agent in both humans and animals.

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  • IPEN-DOC 23931

    FAINTUCH, B.L. ; OLIVEIRA, E.A. ; TARGINO, R.C.; MORO, A.M.. Radiolabeled NGR phage display peptide sequence for tumor targeting. Applied Radiation and Isotopes, v. 86, p. 41-45, 2014. DOI: 10.1016/j.apradiso.2013.12.035

    Abstract: The asparagine-glycine-arginine (NGR) peptide sequence found by phage display, was radiolabeled with technetium-99m and tested in different tumor models. Similar uptake occurred with ovarian and lung tumor cells. Biodistribution of the radiotracer revealed predominant renal excretion with more substantial uptake in animals bearing ovarian tumor cells. In contrast imaging studies indicated better visualization for lung tumor. NGR peptide was characterized as a promising diagnostic candidate, particularly for lung cancer. Improvements are envisaged using NGR combined with RGD as a heterodimer molecule.

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  • IPEN-DOC 23930

    OLIVEIRA, ERICA A. de ; LAZOVIC, JELENA; GUO, LEA; SOTO, HORACIO; FAINTUCH, BLUMA L. ; AKHTARI, MASSOUD; POPE, WHITNEY. Evaluation of magnetonanoparticles conjugated with new angiogenesis peptides in intracranial glioma tumors by MRI. Applied Biochemistry and Biotechnology, 2017. DOI: 10.1007/s12010-017-2443-2

    Abstract: Angiogenesis plays a critical role in progression of malignant gliomas. The development of glioma-specific labeling molecules that can aid detection and visualization of angiogenesis can help surgical planning and improve treatment outcome. The aim of this study was to evaluate if two peptides (GX1 and RGD-GX1) linked to angiogenesis can be used as an MR-imaging markers of angiogenesis. MR imaging was performed in U87 glioblastoma-bearing NOD-SCID mice at different time points between 15 and 120 min post-injection to visualize particle distribution. GX1 and RGD-GX1 exhibited the highest accumulation in U87 glioblastoma at 120 min post i.v. administration. GX1-conjugated agents lead to higher decrease in transverse relaxation time (T2) (i.e., stronger contrast enhancement) than RGD-GX1-conjugated agents in U87 glioblastoma tumor model. In addition, we tested if U87-IDH1R132 mutated cell line had different pattern of GX1 or RGD-GX1 particle accumulation. Responses in U87-IDH1WT followed a similar pattern with GX1 contrast agents; however, lower contrast enhancement was observed with RGD-GX1 agents. The specific binding of these peptides to human glioblastoma xenograft in the brain was confirmed by magnetic resonance imaging. The contrast enhancement following injection of magnetonanoparticles conjugated to GX1 peptide matched well with CD31 staining and iron staining.

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  • IPEN-DOC 23929

    OLIVEIRA, ERICA A. de ; FAINTUCH, BLUMA L. ; TARGINO, ROSELAINE C.; MORO, ANA M.; MARTINEZ, RAQUEL C.R.; PAGANO, ROSANA L.; FONOFF, ERICH T.; CARNEIRO, CAMILA de G.; GARCEZ, ALEXANDRE T.; FARIA, DANIELE de P.; BUCHPIGUEL, CARLOS A.. Evaluation of GX1 and RGD‑GX1 peptides as new radiotracers for angiogenesis evaluation in experimental glioma models. Amino Acids, v. 48, n. 3, p. 821-831, 2016. DOI: 10.1007/s00726-015-2130-y

    Abstract: Gliomas are the most common type among all central nervous system tumors. The aggressiveness of gliomas is correlated with the level of angiogenesis and is often associated with prognosis. The aim of this study is to evaluate the novel GX1 peptide and the heterodimer RGD-GX1 radiolabeled with technetium-99m, for angiogenesis detection in glioma models. Radiolabeling and radiochemical controls were assessed for both radioconjugates. In vitro binding studies in glioma tumor cells were performed, as well as biodistribution in SCID mice bearing tumor cells, in order to evaluate the biological behavior and tumor uptake of the radiocomplexes. Blocking and imaging studies were also conducted. MicroSPECT/ CT images were acquired in animals with experimentally implanted intracranial tumor. Open field activity was performed to evaluate behavior, as well as perfusion and histology analysis. The radiochemical purity of both radiotracers was greater than 96 %. In vitro binding studies revealed rather similar binding profile for each molecule. The highest binding was for RGD-GX1 peptide at 120 min in U87MG cells (1.14 ± 0.35 %). Tumor uptake was also favorable for RGD-GX1 peptide in U87MG cells, reaching 2.96 ± 0.70 % at 1 h p.i. with 47 % of blocking. Imaging studies also indicated better visualization for RGD-GX1 peptide in U87MG cells. Behavior evaluation pointed brain damage and histology studies confirmed actual tumor in the uptake site. The results with the angiogenesis seeking molecule 99mTc-HYNIC-E- [c(RGDfk)-c(GX1)] were successful, and better than with 99mTc-HYNIC-PEG4-c(GX1). Future studies targeting angiogenesis in other glioma and nonglioma tumor models are recommended.

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  • IPEN-DOC 23928

    SCHIPER, LUIS; FAINTUCH, BLUMA L. ; BADARO, ROBERTO J. da S.; OLIVEIRA, ERICA A. de ; CHAVEZ, VICTOR E.A.; CHINEN, ELISANGELA; FAINTUCH, JOEL. Functional investigation of bone implant viability using radiotracers in a new model of osteonecrosis. Clinics, v. 71, n. 10, p. 617-625, 2016. DOI: 10.6061/clinics/2016(10)11

    Abstract: OBJECTIVES: Conventional imaging methods are excellent for the morphological characterization of the consequences of osteonecrosis; however, only specialized techniques have been considered useful for obtaining functional information. To explore the affinity of radiotracers for severely devascularized bone, a new mouse model of isolated femur implanted in a subcutaneous abdominal pocket was devised. To maintain animal mobility and longevity, the femur was harvested from syngeneic donors. Two technetium-99m-labeled tracers targeting angiogenesis and bone matrix were selected. METHODS: Medronic acid and a homodimer peptide conjugated with RGDfK were radiolabeled with technetium-99m, and biodistribution was evaluated in Swiss mice. The grafted and control femurs were evaluated after 15, 30 and 60 days, including computed tomography (CT) and histological analysis. RESULTS: Radiolabeling achieved high (>95%) radiochemical purity. The biodistribution confirmed good blood clearance 1 hour after administration. For Tc-99m-hydrazinonicotinic acid (HYNIC)-E-[c(RGDfK)(2), remarkable renal excretion was observed compared to Tc-99m-methylene diphosphonate (MDP), but the latter, as expected, revealed higher bone uptake. The results obtained in the control femur were equal at all time points. In the implanted femur, Tc-99m-HYNIC-E-[c(RGDfK)(2) uptake was highest after 15 days, consistent with early angiogenesis. Regarding Tc-99m-MDP in the implant, similar uptake was documented at all time points, consistent with sustained bone viability; however, the uptake was lower than that detected in the control femur, as confirmed by histology. CONCLUSIONS: 1) Graft viability was successfully diagnosed using radiotracers in severely ischemic bone at all time points. 2) Analogously, indirect information about angiogenesis could be gathered using Tc-999m-HYNIC-E-[c(RGDfK)(2). 3) These techniques appear promising and warrant further studies to determine their potential clinical applications.

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  • IPEN-DOC 21229

    FAINTUCH, BLUMA L. ; OLIVEIRA, ERICA A. ; NUNEZ, EUTIMIO G.F. ; MORO, ANA M.; NANDA, P.K.; SMITH, CHARLES J.. Comparison of two peptide radiotracers for prostate carcinoma targeting. Clinics, v. 67, n. 2, p. 163-170, 2012. DOI: 10.6061/clinics/2012(02)12

    Abstract: OBJECTIVES: Scintigraphy is generally not the first choice treatment for prostate cancer, although successful studies using bombesin analog radiopeptides have been performed. Recently, a novel peptide obtained using a phage display library demonstrated an affinity for prostate tumor cells. The aim of this study was to compare the use of a bombesin analog to that of a phage display library peptide (DUP-1) radiolabeled with technetium-99m for the treatment of prostate carcinoma. The peptides were first conjugated to S-acetyl-MAG3 with a 6-carbon spacer, namely aminohexanoic acid. METHODS: The technetium-99m labeling required a sodium tartrate buffer. Radiochemical evaluation was performed using ITLC and was confirmed by high-performance liquid chromatography. The coefficient partition was determined, and in vitro studies were performed using human prostate tumor cells. Biodistribution was evaluated in healthy animals at various time points and also in mice bearing tumors. RESULTS: The radiochemical purity of both radiotracers was greater than 95%. The DUP-1 tracer was more hydrophilic (log P = -2.41) than the bombesin tracer (log P = -0.39). The biodistribution evaluation confirmed this hydrophilicity by revealing the greater kidney uptake of DUP-1. The bombesin concentration in the pancreas was greater than that of DUP-1 due to specific gastrin-releasing peptide receptors. Bombesin internalization occurred for 78.32% of the total binding in tumor cells. The DUP-1 tracer showed very low binding to tumor cells during the in vitro evaluation, although tumor uptake for both tracers was similar. The tumors were primarily blocked by DUP- 1 and the bombesin radiotracer primarily targeted the pancreas. CONCLUSION: Further studies with the radiolabeled DUP-1 peptide are recommended. With further structural changes, this molecule could become an efficient alternative tracer for prostate tumor diagnosis.

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  • IPEN-DOC 21321

    GOTARDO, A.T.; LIPPI, L.L.; VIOLIN, K.B. ; GORNIAK, S.L.. Effects of ipomoea carnea in pla-cental tissue. Evaluation in rodents and ruminants. Applied Research in Toxicology, v. 1, 1, p. 122-122, 2015.

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  • IPEN-DOC 21244

    TEIXEIRA, BERNARDO A.N.; BLUM, JOSE R.C.; BRESAOLA JUNIOR, RUBEN; OHBA, MARCIA; SALVI, CLEBER E.R.; GONÇALVES, CRISTINA; OLIVEIRA, DANIELLA P. de; LIMA, FABIANA A.S.; KUMMROW, FABIO; ALVES, IVANIO R.; MELLO, GABRIELA S.L. de; SPRANEMBERG, GILSON; UMBUZEIRO, GISELA de A.; CRIVELARO, HUMBERTO; GOBBI, JOSE E.; AMEDI, KLEBER V.; DI BERNARDO, LUIZ; MORIBE, MARCIA; PIRES, MARIA A.F. ; SATO, MARIA I.Z.; COTRIM, MARYCEL ; DAMATO, MURILO; SILVERIO, PATRICIA F.; SARCINELLI, PAULA de N.; CASTAGNARI, PAULO F.F.; ZAGATTO, PEDRO A.; MONTEIRO, REGINA; SOUZA, ROSEANE M.G.L. de; KUNO, RUBIA; BARROS, SILVIA B. de; FERRARETTO, TANIA R.G.; TOMINAGA, MARIA; RODRIGUES, ANDRE L.G.; FERNA, KHALIL. Subsídios para o Padrão Químico: critérios de qualidade de água para substâncias químicas. Revista ABES-SP, v. 1, n. 1, p. 20-43, 2010.

    Observação: Coordenadoras do Grupo de Trabalho Químico: Gisela de Aragão Umbuzeiro e Patrícia Ferreira Silvério

    Abstract: As substâncias químicas para as quais se estabelecem critérios de qualidade de água são aquelas que têm probabilidade de ocorrência no país ou região e são capazes de causar efeitos adversos ou desconforto aos seres vivos. Estas substâncias podem estar presentes na água devido a características geológicas (naturais) ou por fontes de contaminação. A derivação desses critérios depende da informação obtida em ensaios toxicológicos adotada pelo país chamado de Ingresso Diário Tolerável (TDI) ou Dose de Referência (RfD) e do cenário de exposição, como peso corpóreo, consumo de água, porcentagem de ingresso via água. Também é necessário considerar a viabilidade técnica e econômica de se quantificar quimicamente a substância na água bem como de removê-la aos níveis desejados. Os critérios são transformados em padrões legais após análise dos gestores públicos. A TDI ou RfD, que nada mais é do que a quantidade que pode ingressar no organismo na qual não se espera a ocorrência de efeito adverso, expressa em mg de substância por Kg de peso por dia (mg/Kg/dia). Ela é calculada utilizando a informação existente sobre a toxicidade da substância – expressa normalmente em Nível de efeito adverso não observado (NOAEL). O NOAEL é obtido através de experimentos com animais e dados epidemiológicos, quando disponíveis. Esse valor é dividido por fatores de incerteza os quais são escolhidos em função da qualidade dos dados disponíveis e variabilidade da resposta inter e intra-espécie, entre outras incertezas. Esses fatores variam usualmente de 10 a 1000, são definidos caso a caso e requerem o julgamento científico de um grupo de especialistas. Os critérios de qualidade de água são geralmente calculados multiplicando-se então a TDI pelo peso corpóreo considerado e pela fração ou porcentagem da TDI associada ao uso da água, divididos pelo consumo de água diário adotado.

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  • IPEN-DOC 21318

    FRANCO, EGBERTO G. ; LINARDI, MARCELO . Catalysts for fuel cell: production and characterization / Catalisadores para células a combustível: produção e caracterização. Revista Ciências Exatas - Journal of Exact Sciences, v. 11, n. 1, p. 89-95, 2005.

    Abstract: Fuel cells technology is one of the most promising technologies for this century to deal with energy sustainability. In order to improve fuel cells performances the search for new electrocatalysts has a great importance in this technology. The colloidal method was chosen to produce the catalysts because a good nanocrystal distribution can be obtained. This method consists in the production of a stable colloid that will be anchored in the support (carbon black Vulcan XC 72 R). The catalysts were investigated by the following techniques: energy dispersive analysis (EDX), X-rays powder diffraction (XRD), X-rays photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM) and polarization curves (Exi). The binary and ternary catalysts systems synthesized in this work were tested for the oxidation of hydrogen, H2/CO mixtures (150 ppm of CO), methanol and ethanol. The catalyst systems produced by the colloidal method presents a highly-dispersed nanocrystals structure with an average size of 2.0 to 3.1 nm. The electrocatalytic activity of the synthesized Pt-Ru-Mo catalysts for reformate and methanol oxidation was evidenced by polarization curves showing a very promising system to be optimized. PtRuDy behavior is compared to state of the art catalysts for direct ethanol fuel cells (DEFC) and should be tested in long term experiments in direct ethanol fuel cells. Only both structural and electrochemical characterization together will result in a detailed understanding of the effect of the structure on the eletrocatalytic activity of catalysts in real fuel cell systems. This work is part of a systematic research of the fuel cell group of IPEN/CNEN-SP and further investigations in the performance of these catalysts will be done. The PtRuMo system could be used for technical applications.

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  • IPEN-DOC 22414

    FRANCO, EGBERTO G.; DANTAS FILHO, PAULO L.; RIBEIRO, CARLOS E.R.; BURANI, GERALDO F.; LINARDI, MARCELO . Proton exchange membrane fuel cell catalyst: synthesis and characterization. In: INTERNATIONAL FUEL CELL SCIENCE, ENGINEERING AND TECHNOLOGY CONFERENCE, 6th, June 16-18, 2008, Denver, Colorado, USA. Proceedings... 2008. p. 1-6.

    Abstract: Fuel cells are a promising technology to deal with energy sustainability, especially for mobility purposes the Proton Exchange Membrane Fuel Cell and hydrogen produced from biomass could be coupled to overcome the amount of CO2 emissions. In order to improve fuel cells performances the search for new electrocatalysts has a great importance in this technology the challenge for a fuel cell catalyst that is less poisoned by CO is one of the most important field in low temperature fuel cell developments that use alcohol and hydrocarbons as primary fuels. In this work PtSm, PtTb, PtDy, PtU, PtRuMo and PtRuDy systems have been synthesized by the colloid method, investigated by the following techniques: X-rays fluorescence analysis (XFA), X-rays powder diffraction (XRD), X-rays photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM), cyclic voltammetry (CV) and polarization curves (Exi). The results obtained in this work shows that PtRuMo is the best choice for direct methanol oxidation. For direct ethanol oxidation the higher activity was found in PtRuDy system. PtU system was investigated and showed an interesting behaviour in ethanol oxidation. After two cycles of H2/O2 and ethanol/O2 the catalyst was able to reach the initial figures on hydrogen/oxygen oxidation which means that no degradation of the catalyst was indentified.

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  • IPEN-DOC 22701

    SENNA, ROQUE M. de ; LINARDI, MARCELO ; CASSIANO, DOUGLAS A.; SANTOS, IVAN ; MOTA, HENRIQUE de S. ; JERONIMO, ROSIMEIRE A.. Mathematical modeling of a 500 We power module composed by a PEM fuel cell combined with a DC-DC enhanced potential output converter. In: INTERNATIONAL CONFERENCE ON INTEGRATED MODELING AND ANALYSIS IN APPLIED CONTROL AND AUTOMATION, 6th, September 19-21, 2012, Wien, Austria. Proceedings... 2012.

    Abstract: This work presents the development of a mathematical modeling of a 500 We PEMFC fuel cell stack (MCC500) system combined with a dc-dc enhanced potential output converter. The MCC500 was developed at IPEN (Nuclear and Energy Research Institute) and the company Electrocell, using only Brazilian technology. Mathematical developments and modeling have been performed, relying on experimental data collected at IPEN laboratory. The first step was to prepare an electrical system (pre-design) for the proposed model, which included the MCC500 parameters, like: membrane ohmic resistance, activation resistance, electric double layer capacitance, open circuit potential, as well as DC-DC converter parameters, like: inductor and transistor switching frequency. Using the obtained parameters and a linear differential equation system with some mathematical manipulations, an electrical system model was determined. Simulations experiments demonstrated that the system was very stable. This toll contribution showed to be very important to generate useful potential for practical purposes, increasing the overall system electrical efficiency.

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  • IPEN-DOC 23926

    NUNES, EDILENE de C.D.; SOUZA, ALANA G. de; COIADO, RENATA D.S.; MOURA, ESPERIDIANA A.B. ; ROSA, DERVAL dos S.. Evaluation of the Poly (Lactic Acid) and calcium carbonate effects on the mechanical and morphological properties in PBAT blends and composites. International Journal of Innovative Science, Engineering & Technology, v. 4, n. 6, p. 313-318, 2017.

    Abstract: In this study, the effect of the organic (poly(lactic acid)) and the inorganic (calcium carbonate (CaCOR3R)) fillers were evaluated in mechanical, thermal, morphological and surface properties of the poly(butylene adipate-co-terephthalate) (PBAT) matrix. Mechanical results showed that the fillers has an influence on the PBAT matrix. The use of CaCOR3R showed higher elongation at break than PLA. However, the blends showed higher elastic modulus, resulting in greater stiffness to the PBAT matrix. Both fillers thermally destabilized the matrix. The morphological properties showed a uniform dispersion of the fillers in the composites and blends, however it is weaker with PLA. Because the PBAT matrix surface is, intrinsically, hydrophobic, the composites presented a smaller degree of hydrophobicity, while the PLA makes the blends hydrophilic.

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  • IPEN-DOC 23927

    OLIVEIRA, EDUARDO S. ; SAMAD, RICARDO E. ; VIEIRA JUNIOR, NILSON D. ; ROSSI, WAGNER de . Ablação seletiva de filme fino de TiN depositado sobre um substrato de WC. In: ABM WEEK; CONGRESSO ANUAL DA ABM, 71.; ENCONTRO NACIONAL DE ESTUDANTES DE ENGENHARIA METALURGICA, DE MATERIAIS E DE MINAS, 16., 26-30 de setembro, 2016, Rio de Janeiro, RJ. Anais... São Paulo: Associação Brasileira de Metalurgia, Materiais e Mineração, 2016.

    Abstract: Devido à possibilidade de se remover quantidades muito pequenas de material de cada vez, a ablação seletiva por laser tem se mostrado um método muito eficaz para remoção de finas camadas de materiais depositados sobre um substrato. Neste trabalho, um laser de femtossegundo foi utilizado para remoção de um filme fino de nitreto de titânio (TiN) depositado sobre um substrato de carboneto de tungstênio (WC), configuração comum em pastilhas de usinagem mecânica, objetivando fornecer um método eficiente para tal processo. Os limiares de ablação do filme e do substrato foram determinados, evidenciando a problemática do trabalho, remover um filme com limiar maior que o substrato. Um sistema de controle de espectrometria de emissão atômica induzida por laser (LIBS) foi testada, com a intenção de controlar em tempo real, a espécie do material ablacionado, mostrando-se muito eficiente. Os parâmetros do laser bem como da varredura de usinagem ainda estão sendo testados para definir a viabilidade do processo.

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  • IPEN-DOC 23925

    SUZIN E SILVA, MIRNA M. ; CARVALHO, ELITA F.U. de ; RIELLA, HUMBERTO G. . Tratamento oxidativo em águas residuais da mineração de urânio com oxidação avançada de ozônio. In: PINHEIRO, ALEX G. (Colab.); SILVA, CARLA M. (Colab.); MENDES, ERLON (Colab.); WAJC, ISAC M. (Colab.); YAMANAKA, MARCOS H. (Colab.); QUADRI, MARINTHO B. (Colab.); SUZIN E SILVA, MIRNA M. (Colab.); GUIMARAES, NATALIA R. (Colab.); MOREIRA JUNIOR, PAULO F. (Colab.); MANFIOLETTI, SUELEN Z. (Colab.)ANGIOLETTO, ELIDIO (Coord.); CARVALHO, ELITA F.U. de (Coord.); BIAZINI FILHO, FRANCISCO L. (Coord.); LAGE FILHO, FREDERICO A. (Coord.); RIELLA, HUMBERTO G. (Coord.); SANTOS, IVAN (Coord.); DEPINTOR, JOSE C. (Coord.); BOSCOV, MARIA E.G. (Coord.); MENASCE, SAMY (Coord.); . Ozônio na recuperação de solos e recursos hídricos contaminados por mineração. Criciúma, SC: UNESC, 2016. p. 71-109.

    Notas de conteúdo: disponível em formato impresso e on-line

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  • IPEN-DOC 23924

    PINHEIRO, ALEX G. (Colab.); SILVA, CARLA M. (Colab.); MENDES, ERLON (Colab.); WAJC, ISAC M. (Colab.); YAMANAKA, MARCOS H. (Colab.); QUADRI, MARINTHO B. (Colab.); SUZIN E SILVA, MIRNA M. (Colab.); GUIMARAES, NATALIA R. (Colab.); MOREIRA JUNIOR, PAULO F. (Colab.); MANFIOLETTI, SUELEN Z. (Colab.)ANGIOLETTO, ELIDIO (Coord.); CARVALHO, ELITA F.U. de (Coord.); BIAZINI FILHO, FRANCISCO L. (Coord.); LAGE FILHO, FREDERICO A. (Coord.); RIELLA, HUMBERTO G. (Coord.); SANTOS, IVAN (Coord.); DEPINTOR, JOSE C. (Coord.); BOSCOV, MARIA E.G. (Coord.); MENASCE, SAMY (Coord.); . Ozônio na recuperação de solos e recursos hídricos contaminados por mineração. 2016. Criciúma, SC: UNESC, 250 p.

    Notas de conteúdo: disponível em formato impresso e on-line

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  • IPEN-DOC 23923

    AQUINO, AFONSO R. de ; PIEDADE, ANA L.F.; BUONGERMINO, CAIO R.P.; SANTOS, DYMES R.A. dos ; ROCHA, ERNESTO D.; KIIPER, FELIPE de M.; PALETTA, FRANCISCO C.; AFFONSO, GUSTAVO S.; BORDON, ISABELLA C.A.C. ; COELHO, JOAQUIM M.S.; ALMEIDA, JOSIMAR R. de; PIDONE, LEANDRO A.; OLIVEIRA, MARIA J.A. de ; RIBEIRO, RENAN B.; YAMAGUISHI, RENATA B. ; MULLER, RENATA M.L.; MATTIOLO, SANDRA R.; BOEMER, VERONICA A.; LA RUBBIA, WALDIR M.. Risco Ambiental. 2017. São Paulo: Blucher, 134 p.

    Notas de conteúdo: disponível em formato impresso e on-line

    Abstract: O estudo do risco está quase sempre relacionado à sua percepção, pois esta, em muitos momentos, determina a maneira como se deve agir, o que tem influenciado a aplicação de novas tecnologias. O reconhecimento de algo como perigoso é influenciado pelo contexto sócio-histórico de cada um e pela percepção individual. Nas últimas décadas, o risco ambiental tem despertado atenção maior da comunidade científica dos governantes e de toda a população por ameaçar diretamente a vida no planeta, afetar o equilíbrio da biodiversidade e a exploração de recursos renováveis, bem como por contribuir para a alteração global do clima. Em função dos riscos acarretados pelas mudanças climáticas, acordos mundiais de proteção ao clima foram adotados, principalmente, a partir da década de 1980. A experiência na avaliação de risco vem sendo aplicada a situações em que a contaminação química por produtos perigosos é o principal agente e está associada à saúde humana e ao meio ambiente. Neste livro, os conceitos de risco são apresentados. Ele abrange desde riscos ambientais urbanos e empresariais, até riscos envolvendo aparelhos eletrônicos, transporte de produtos perigosos e biomarcadores. Além disso, aqui, a gestão de riscos é apresentada junto de uma metodologia de análise que visa estimar a probabilidade de ocorrência de incidentes e seus possíveis impactos, estudando medidas para evitar, reduzir, assumir ou transferir os riscos.

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  • IPEN-DOC 23922

    AQUINO, AFONSO R. de ; LANGE, CAMILA N. ; LIMA, CLARICE M. de; AMORIM, EDUARDO P. de; PALETTA, FRANCISCO C.; FERREIRA, HENRIQUE P. ; BORDON, ISABELLA C.A.C. ; ALMEIDA, JOSIMAR R. de; GOMES, MARCO A.U.G.; ZAMPIERI, MARIA C.T. ; OLIVEIRA, MARIA J.A. de ; CORREIA JUNIOR, PAULO de A.C.; SOUZA, RENATA R. de; MATTIOLO, SANDRA R.; RODRIGUES, SILVIA G.. Vulnerabilidade Ambiental. 2017. São Paulo: Blucher, 112 p.

    Notas de conteúdo: disponível em formato impresso e on-line

    Abstract: A questão ambiental é ampla e complexa, mas aos poucos tem sido colocada em pauta nos mais diversos setores sociais e, dessa maneira, a humanidade caminha para um desenvolvimento ambiental. Um mecanismo adotado para alcançar esse desenvolvimento é subdividir o tema em diversos aspectos, como gestão ambiental, riscos, políticas e práticas ambientais, sustentabilidade e preservação. É importante lembrar que todas essas subdivisões interagem entre si e que a abordagem de vulnerabilidade é mais uma nota que visa auxiliar a composição da complexa sinfonia. Este livro foi estruturado de forma a discutir as vulnerabilidades natural, ambiental e social, proporcionando ao leitor uma breve introdução ao conceito de vulnerabilidade. Além disso, são apresentados aqui os indicadores e índices de vulnerabilidade ambiental, ferramentas cruciais para avaliar quanto o ser humano está vulnerável ou quão fragilizado ele torna o meio em que se encontra. Isso ajuda a identificar as comunidades-alvo em que são necessárias medidas proativas, principalmente para prevenir as devastadoras consequências de eventos extremos que possam vir a ocorrer. O livro tem seu enfoque nas interfaces entre vulnerabilidade e mudanças climáticas, assim como entre ações humanas e poder público. Isso porque a avaliação da vulnerabilidade pode se tornar o núcleo de políticas públicas de âmbito regional, nacional ou internacional, funcionando como um “sistema de alerta precoce”. Da mesma maneira, a análise de vulnerabilidade ambiental pode servir como guia para cientistas e para o poder público, direcionando-os a uma melhor gestão dos recursos naturais.

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  • IPEN-DOC 23921

    SANTOS, S.C. ; YAMAGATA, C. ; ACCHAR, W.; MELLO-CASTANHO, S.R.H. . Yttria nettings by replica processing. Materials Science Forum, v. 798-799, p. 687-690, 2014. DOI: 10.4028/www.scientific.net/MSF.798-799.687

    Abstract: In the context of green economy supported and widespread by United Nations (UN), the renewable energy sources are the unique mechanism to universalize the access to energy. Face to this strategic direction of change is essential to develop efficient components for saving energy such as porous ceramics that associate light with mechanical strength. Thus, the objective of this work concerns to improve the homogeneity of porous size distribution of yttria porous ceramics by evaluating their morphology during replica processing. Samples were subject to immersion into 30vol% yttria aqueous suspensions during an interval from 1min to 120min and sintered through careful thermal conditions. Based on the results, the weight and morphology of porous structure samples were directly influenced by immersion time, whereby intervals from 30-120min showed the best final products.

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  • IPEN-DOC 23920

    RODRIGUES, L.R.; DIAS, C.G.B.T.; CERAGIOLI, H.J.; RODAS, A.C.D. ; MONTEIRO, F.J.M.; ZAVAGLIA, C.A.C.. FTIR analysis and cytotoxicity test of titanium dioxide nanoparticles. Key Engineering Materials, v. 493-494, p. 768-774, 2012. DOI: 10.4028/www.scientific.net/KEM.493-494.768

    Abstract: Titanium dioxide is a material widely used in electronics industry and little explored in the biomedical area, which is the objective of this work. Nowadays one can find surgical instruments coated with thin films that have bactericidal properties when they are activated in the presence of ultraviolet light. For crystalline phase control TiO2 was calcinated at 500°C. The crystallite mean size for sample calcinated at 500°C was 27nm. With the results of cytotoxicity it is possible to say that biomedical applications are possible. Electron microscopy images showed nanoparticles obtained by sol-gel process and the compounds were identified by FTIR analysis. Raman spectroscopy confirmed the existence of anatase titania phase and X-ray diffraction showed this material to be composed of a crystalline phase. X-ray fluorescence identified chemical contaminants.

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  • IPEN-DOC 23919

    MEIRA, CAMILA R. de; GOMES, DANNYLO T.; BRAGA, FRANCISCO J.C. ; PURQUERIO, BENEDITO de M.; FORTULAN, CARLOS A.. Direct manufacture of hydroxyapatite scaffolds using blue laser. Materials Science Forum, v. 805, p. 128-133, 2015. DOI: 10.4028/www.scientific.net/MSF.805.128

    Abstract: The study deals with the direct manufacturing of hydroxyapatite scaffolds using selective polymerization of the slurry liquid phase. The bovine hydroxyapatite has great similarity with the human bone structure, making it able for a direct connection with the bone tissues. This study aims to obtain scaffolds using a new technique of rapid prototyping, obtained by polymerization of acrylic resin (liquid phase of slurry) by ultraviolet light present in a range of the band spectrum emitted by the blue laser light. Sub-micrometer hydroxyapatite was obtained by the calcination and grinding of bovine bone in a vibratory mill. Mixtures of hydroxyapatite and resin were prototyped in three-dimensional pieces and sintered afterword and subjected to blue laser emission path directed in a CNC equipment. Grounded particles obtained in the grinding vibratory mill, with equivalent diameter of 0.35 microns, were reactive enough to compensate the low green densification bellow 50 vol%. Polymerization tests realized indicated that the incidence of the laser with fluency of 170 mW.s/mm2 promoted the curing of the 0.5 mm diameter pieces in depth about 0.5 mm, which allowed the prototyping of the scaffolds with sufficient mechanical strength for handling.

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  • IPEN-DOC 23918

    GODOY, ANA L.E. ; BRESSIANI, J.C. ; BRESSIANI, A.H. de A. . Influence of addition of polysiloxane on sintering. Materials Science Forum, v. 798-799, p. 696-702, 2014. DOI: 10.4028/www.scientific.net/MSF.798-799.696

    Abstract: The effects of adding polysiloxane (polymethylsilsesquioxane) on the sintering behavior, microstructure and hardness of alumina ceramics have been studied. The sintering kinetics of coldpressed specimens was studied by dilatometry. Polymer sintering aid was used for alumina based ceramics sintered at 1650º C. The sintered materials were characterized using helium picnometry, x-ray diffraction analysis, scanning electron microscopy, transmission electron microscopy, energy dispersive x-ray spectrometry and Vickers indentation. The addition of polymethylsilsesquioxane yielded alumina/Si2Al4O4N4 composites. The sintering studies in nitrogen atmosphere (1650 °C/1h) revealed that the highest densities were obtained for specimens with 1wt% PMS. The materials prepared with ceramic polymer precursors had higher hardness than the standard specimen. The preparation of ceramic composites using small amounts of polymer precursors is suited for alumina based ceramics. The processing route being simple, it has the potential to enable the manufacture of complex shaped pieces.

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  • IPEN-DOC 23917

    BRITO, VERA L.O. de; CUNHA, STEPHANIE A.; UCHOAS, ANA P.R.; ARAUJO, FABIANA F. de; NUNES, CRISTINA B.; GENOVA, LUIS A. . Evaluation of the sinterability of copper-substituted ferrites by means of dilatometric thermal analysis. Materials Science Forum, v. 805, p. 254-259, 2015. DOI: 10.4028/www.scientific.net/MSF.805.254

    Abstract: Cobalt and cobalt-manganese spinel ferrites have magnetostrictive properties suitable for application in magneto-electric and magneto-mechanical transducers. In this work, coppersubstituted ferrites of these compositions were processed by means of the ceramic method and their sinterabilities were evaluated by dilatometric thermal analyses. The results obtained suggest that copper affects the solid-state reactions for the spinel formation and lowers the required sintering temperature for the ferrites. However, the densification obtained with sintering of the coppersubstituted ferrites at 950 oC for 6h was only 64%, which indicates that further adjustments on the processing route must be made in order to obtain higher densities.

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  • IPEN-DOC 23902

    MOREIRA, T.M.; SEO, E.S.M. . Corn leaf fibers preparation and characterization for composite obtention. Materials Science Forum, v. 881, p. 271-276, 2017. DOI: 10.4028/www.scientific.net/MSF.881.271

    Abstract: There is a global trend in seeking plant fibers to replace the synthetic fibers to obtain reinforced composites, aiming the use of renewable resources. In this context, this paper objective is to develop the process of corn leaf fibers preparations , characterize and adapt them for applications in the construction industry. Corn leaves were dried in room temperature, treated by mercerising (chemical process of attack on the fiber surface making it rough); then neutralized with acid solution and washed in running water. The characterization of the corn leaf fibers was performed by X-ray diffraction, X-ray fluorescence Spectrometry, Scanning Electron Microscopy, Thermogravimetry, specific mass and Fourier Transform Infrared Spectrometry. The mercerizing treatment was effective, because the corn fibers have similar characteristics to synthetic fibers, leading to a possibility of new technological uses, besides the application in gas pipe manufacturing.

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  • IPEN-DOC 23916

    REIS, A.S.; GUILHEN, S.N.; ORTIZ, N. . Water treatment adsorption and UV photodecomposition processes for antibiotics: Cephalexin and Amoxicillin. In: IWA LEADING EDGE CONFERENCE ON WATER AND WASTEWATER TECHNOLOGIES, 13th, June 13-16, 2016, Jerez de la Frontera, Spain. Resumo expandido... 2016.

    Abstract: The developed of water treatment process to adsorb and decompose the pharmaceutical antibiotic amoxicillin and cephalexin using iron oxide –hematite, quitosan and UV radiation. The equilibrium time te= 180 min and the removal percentage for amoxicillin was 90% similar with those found in literature. In same experimental parameters the Cephalexin removal percentage was 25% some parameter have been alter to promote the photodecomposition such antibiotic mixture. The results confirm an indicate the possible use of UV photodecomposition reaction as a promising water treatment to treat, adsorb and decompose antibiotic residues in the environment.

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  • IPEN-DOC 23915

    TOMINAGA, F.K.; BOIANI, N.F.; SOLE, S.V.D.; BORRELY, S.I. . Efeito da irradiação com feixe de elétrons na degradação e toxicidade de fármacos em solução aquosa: cloridrato de fluoxetina, diclofenaco de sódio e mistura de ambos. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 722-723.

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  • IPEN-DOC 23914

    SOLE, S.V.D.; TOMINAGA, F.K.; BOIANI, N.F.; BORRELY, S.I. . Avaliação de toxicidade do efluente têxtil antes e após a irradiação com feixe de elétrons. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 1239-1240.

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  • IPEN-DOC 23913

    SANTOS, D.R.A.; PUSCEDDU, F.H.; FRANCO, H.R.; NOBRE, C.R.; MORENO, B.B.; ROSA, J.L.; CORTEZ, F.S.; PEREIRA, C.D.S.; CESAR, A.; SANTOS, A.R.; ROGERO, S.O. ; ROGERO, J.R. . Análise da citotoxicidade de sedimentos marinhos contaminados com fluoxetina para mexilhões Mytella charruana. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 841-842.

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  • IPEN-DOC 23912

    SANTANA, N.S.; TOMINAGA, F.K.; COLOMBO, R.; BORRELY, S.I. ; NOLASCO, M.A.. Avaliação de remoção de toxicidade de esgoto sanitário tratado por meio de sistemas descentralizados. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 1647-1649.

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  • IPEN-DOC 23911

    PUSCEDDU, F.H.; MARANHO, L.A.; ABESSA, D.M.S.; NOBRE, C.R.; SANTOS, D.R.A.; PEREIRA, C.D.S.; CORTEZ, F.S.; SANTOS, A.R.; ROGERO, J.R. ; CESAR, A.. Biomarcadores de exposição e efeito em mexilhões Mytella charruana expostos a sedimentos marinhos contaminados com Triclosan, Ibuprofeno e 17α-ethinylestradiol. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 768-770.

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  • IPEN-DOC 23910

    NAKATSUBO, M.A.S.; SARKIS, J.E.S. . Bioacessibilidade in vitro como ferramenta para avaliar e quantificar metais potencialmente toxicos nos pescados. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 144-146.

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  • IPEN-DOC 23909

    FRANCO, H.R.; SANTOS, D.R.A.; PUSCEDDU, F.H.; CORTEZ, F.S.; PEREIRA, C.D.S.; CESAR, A.; SANTOS, A.R.; ROGERO, S.O. ; ROGERO, J.R. . Avaliação do efeito crônico de sedimento marcado com fluoxetina sobre o desenvolvimento embriolarval de Echinometra lucunter. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 831-832.

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  • IPEN-DOC 23908

    DUARTE, L.F.A.; OSTANIK, P.A.G.; PAÇO, M.S.; FERREIRA, B.D.; EVANGELISTA, M.V.; MENDES, A.L.A.; NOBRE, C.R.; SEMMLER, M.G.M.C.; MOREIRA, E.G. ; PEREIRA, C.D.S.. Tolerância biológica, efeitos subletais e bioacumulação em Ucides cordatus (LINNAEUS, 1763) expostos ao metal cádmio. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 247-257.

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  • IPEN-DOC 23907

    CONICELLI, B.P.; SCHENBERG, A.C.G.; BIONDO, R.; SILVA, F.A.; HORTELLANI, M.A. ; SARKIS, J.E.S. . Efeito do PH na biossorção de HG(II) NA Cupriavidus metallidurans recombinante. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 1622-1623.

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  • IPEN-DOC 23906

    CAVALCANTE, A.K.; MARTINI, G.A.; MAZIERO, J.S.; CARDOSO, K.D.; SANGES; FERREIRA, M.V.A.L.; SANGES, G.; ROGERO, S.O. ; ROGERO, J.R. . Avaliação das deformidades em larvas de Danio rerio após exposição à substância de referência (NACL). In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 1423-1424.

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  • IPEN-DOC 23870

    BOIANI, N.F.; SOLE, S.V.D.; TOMINAGA, F.K.; BORRELY, S.I. . Remoção da toxicidade de fármacos em solução aquosa empregando irradiação com feixe de elétrons. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 14., 07-10 de setembro, 2016, Curitiba, PR. Resumo expandido... Campinas: Sociedade Brasileira de Ecotoxicologia, 2016. p. 716-718.

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  • IPEN-DOC 23884

    ROSTELATO, MARIA E.C.M. ; ZEITUNI, CARLOS A. ; MOURA, JOAO A. ; FEHER, ANSELMO ; COSTA, OSVALDO L. da ; MOURA, EDUARDO S. de ; SOUZA, CARLA D. de ; KARAM JUNIOR, DIB . Estudo, desenvolvimento e produção de fontes radioativas para radioterapia. In: ENCONTRO NACIONAL DE FÍSICA NA INDÚSTRIA, 1., 04-05 de abril, 2013, São Carlos, SP. Resumo... São Paulo: Sociedade Brasileira de Física, 2013. p. 44-44.

    Abstract: Desde 1997, o IPEN-CNEN/SP por meio do seu “Laboratório de Produção Fontes para Radioterapia - LPFR”, produz fontes radioativas de Irídio-192, na forma de fi os, e fornece aos hospitais para utilização em radioterapia de baixa taxa de dose (LDR). Está em fase de conclusão, a montagem de uma instalação para a produção de fontes radioativas de Iodo-125, na forma de sementes, para uso em tratamento de câncer de próstata, oftálmico e cerebral. Essa instalação consiste de três glove-box (uma caixa estanque). A primeira, semi-automatizada, é a reação do iodo radioativo com o núcleo de prata que forma a semente. É a parte do processo que apresenta maior risco, tendo diversos sistemas redundantes de segurança como duplo fi ltro, depressão de ar e proteção física. A segunda é a solda da cápsula de titânio que envolve esse núcleo. Essa parte é inteiramente automatizada e o controle visual da qualidade da solda é feito pelo operador através de um sistema de câmaras. A terceira fase é o controle de qualidade, totalmente automatizado, que garante a estanqueidade das sementes prontas de acordo com a norma ISO 9978 - “Sealed Radioactive Sources-Leak Test Methods”. Como fase extra a dosimetria da semente está em desenvolvimento. Ela engloba o estudo de diversos parâmetros que auxiliam no cálculo da dose exata que o paciente recebeu ao utilizar esse tratamento. O grupo espera a conclusão para 2014. Atualmente encontra-se em desenvolvimento e implementação um laboratório para produção de fontes de Irídio-192, na forma de “pellets”, para utilização em radioterapia de alta taxa de dose (HDR). Estas fontes são importadas acarretando altos custos para a sociedade. Estas fontes são usadas para tratar vários tipos de câncer. Por exemplo: colo de útero, mama, pulmão e outros. A produção local de fontes radioativas para radioterapia reduzirá sensivelmente seus custos, permitindo um aumento do número de pacientes tratados no país. A proteção implementada no caso do Irídio-192 é maior quando comparada com o iodo-125. O primeiro isótopo apresenta energia de emissão entre 0,137 a 0,65 MeV enquanto o segundo 0,03MeV. Por essa razão o irídio será manipulado em hot cell (célula quente) que são enormes estruturas totalmente revestidas de chumbo pesando aproximadamente 25 toneladas. O operador usará um telemanipulador e o sistema do ar será totalmente selado. O vidro para visualização contém chumbo na formulação. Como resultado o operador leva praticamente nenhuma dose de radiação. O grupo espera a conclusão do novo prédio em 2018.

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  • IPEN-DOC 23871

    FERRAZ, CAROLINE C.; LUGAO, ADEMAR B. ; BUCIO, EMILIO. Radiation-grafting of thermo- and pH-sensitive poly(N-vinylcaprolactam-co-acrylic acid) on silicone rubber and polypropylene films. In: AMERICAN CHEMICAL SOCIETY NATIONAL MEETING, 245th, April 07-11, 2013, New Orleans, Louisiana, USA. Abstract... 2013.

    Abstract: This work focuses on the effect of gamma-ray radiation conditions on the stimuli-responsiveness of polypropylene (PP) films and silicone (SR) rubber substrates grafted with N-vinylcaprolactam (NVCL) and acrylic acid (AAc). PP films and SR rubber were weighed and placed into glass ampoules and exposed to Co-60 gamma-source (Gammabeam 651 PT, MDS Nordion) in the presence of air at room temperature, at dose rate around 12 kGy/h and dose between 5 and 70 kGy. Solutions of NVCL and AAc (1/1, v/v), 50 % monomer concentration (v/v) in toluene were added to samples, the ampoules were degassed by repeated freeze-thaw cycles (5 times per 20 min) and sealed. The ampoules were heated at 60 and 70 °C at reaction time per 12 h. To extract the residual monomer and homopolymer formed during the grafting, the samples were soaked in ethanol for 24 h and then in distilled water, followed by drying under vacuum to constant weight. Samples where characterized by FTIR-ATR, DSC, swelling, LCST, and pH critical point.

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  • IPEN-DOC 23900

    STEFFENS, D.; SANTI, B.T.; LUCO, D.P.; MATHOR, M.B. ; PRANKE, P.. Co-cultivation of mesenchymal stem cells and skin keratinocytes on electrospun scaffolds. In: TERMIS-AM CONFERENCE, December 13-16, 2014, Washington, DC, USA. Abstract... 2014. p. S-89 - S-89.

    Abstract: Available treatments in skin regeneration are insufficient for promoting healing. This study has aimed to produce a cutaneous substitute combining mesenchymal stem cells (MSCs), keratinocytes and a PDLLA biomaterial, constructed by electrospinning, into 3 groups: 1) PDLLA, 2) PDLLA/NaOH, scaffolds with hydrolyzed surfaces and 3) PDLLA/Lam, hydrolyzed and with laminin binding. The MSCs were seeded at the bottom of the scaffolds. After 24 hours, skin keratinocytes were seeded at the top. The scaffolds were characterized by morphology, fiber diameter, cell adhesion on the day of the seeding and viability on days 7, 14 and 21. As a result, the scaffolds presented randomly distributed, well formed fibers. The fiber diameter for all the groups was 4.58 mmfor the largest fibers and 574 nm for the smallest. The pore sizes of the scaffolds obtained were approximately 27.5 mm and 3.44 mm, for the largest and smallest fibers, respectively. In biological analysis, cell adhesion was greater in the PDLLA/Lam scaffolds with absorbance of 2.268 – 0.494, in comparison with 1.264 – 0.473 for the PDLLA and 1.159 – 0.120 for the PDLLA/NaOH scaffolds. There was an improvement in the number of viable cells from day 7 to day 21 of analysis. In general, the PDLLA/Lam group showed the best results for cell adhesion and viability tests. In conclusion, the PDLLA scaffolds, mainly the PDLLA/Lam groups, showed good results for the co-cultivation of the cells, with good cell adhesion and the presence of viable cells. Therefore, these scaffolds show promise to be suitable biomaterials for use in tissue engineering.

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  • IPEN-DOC 23899

    PRANKE, PATRICIA; STEFFENS, DANIELA; SANTI, BRUNA; LUCO, DAYANE P. ; MATHOR, MONICA B. . Evaluation of electrospun matrices for the co-cultivation of mesenchymal stem cells and skin keratinocytes. In: INTERNATIONAL SOCIETY FOR STEM CELL RESEARCH, 12th, June 18-21, 2014, Vancouver, Canada. Proceedings... 2014.

    Abstract: The regeneration of skin is an important field for tissue engineering. Currently available treatments are insufficient to prevent scar formation and promote healing of the patient, especially in large burns and chronic wounds. Due to the great need for skin substitutes with the ability of regenerating large amounts of skin, as well as the lack of an ideal replacement, the current study has aimed to produce a cutaneous substitute with a PDLLA polymer as a biomaterial. These, in turn, must be able to serve as a suitable support for cellular growth for the period of time required for tissue regeneration. For this purpose, scaffolds were constructed by the electrospinning technique and divided into 3 groups: 1) PDLLA matrices, 2) PDLLA/NaOH, which were PDLLA scaffolds hydrolyzed with a solution of NaOH 0.75M and 3) PDLLA/Lam, also hydrolyzed with NaOH and in which the protein laminin was linked by covalent binding. They were all constructed with 2 different fiber diameters, with the smallest at the top of the scaffold. These scaffolds were characterized by morphology and fiber diameter and their hydrophilicity or hydrophobicity features. Mesenchymal stem cells were then seeded onto the bottom of the scaffold and, after 24 hours, skin keratinocytes were seeded on the other side. This procedure was performed in all the groups. The groups were evaluated for cell adhesion on the day of the seeding and on days 7, 14 and 21 for viability with WST-8 assay. From day 7, the scaffolds were submitted to an air/liquid system of culture. As a result, the scaffolds presented well formed fibers which were randomly distributed. The treatment of the matrices with NaOH for 15 minutes did not substantially affect the structure of the fibers, but it was enough to hydrophilize the surface of the biomaterials, which is necessary for laminin linkage. The fiber diameter for all the groups was 4.58 μm for the largest fibers and 574 nm for the smallest. The pore size of the scaffolds obtained were approximately 27.5 μm and 3.44 μm, respectively, for the largest and smallest fibers. The linkage of the laminin was confirmed by immunofluorescence assay. For the biological analysis, cell adhesion was greater in the PDLLA/Lam scaffolds with absorbance of 2.268± 0.494, in comparison with 1.264±0.473 for the control (PDLLA scaffold) and 1.159±0.120 for the PDLLA/NaOH scaffold. On day 7 of the viability analysis, the absorbance for the PDLLA scaffold was 1.148±0.411, the PDLLA/NaOH group was 1.380±0.501 and the PDLLA/Lam was 1.990±0.255. On day 14, the absorbance for groups 1, 2 and 3 were 1.032±0.169, 0.755±0.016, and 1.636±0.313, respectively. On day 21, the results were 2.204±0.317, 1.437±0.024, 2.811±0.477 respectively for groups 1, 2, and 3. In general, in terms of the biological analysis, the PDLLA/Lam group showed the best results for cell adhesion and viability tests. Histological analysis is being processed for greater understanding of the behavior of the cells interacted within the scaffolds. In conclusion, the PDLLA scaffolds, mainly the PDLLA/Lam groups, showed good results for the cocultivation of the cells, with good cell adhesion and the presence of viable cells. These biomaterials were capable of providing support for the growth of the cells, which was observed by the increase in the absorbance over time. Therefore, although histological analysis is still in progress, these scaffolds promise to be suitable biomaterials for use in tissue engineering.

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  • IPEN-DOC 23903

    SCAGLIUSI, SANDRA R. ; CARDOSO, ELIZABETH C.L. ; LUGAO, ADEMAR B. . Radiation effects on crosslinking of butyl rubber compounds. In: TMS ANNUAL MEETING AND EXHIBITION, 146th, February 26 - March 2, 2017, San Diego, CA, USA. Proceedings... 2017.

    Abstract: When butyl rubbers are subjected to high energy radiation, they form easy free radicals that initiate various chemical reactions. These reactions alter the molecular distribution of irradiated rubbers by crosslinking or scission affect their physical and mechanical properties. This work aims to the analysis of effect induced by -exposure on the crosslinking density in butyl rubbers by swelling measurements accomplished before and after irradiation at 25, 50, 100 and 200 kGy, with further evaluation of crosslinking density accomplished by Flory-Rehner equation; this is a proper procedure for the qualification of radiation resistance. It can be noticed that changes in material structure was due to build-up of new three-dimensional network in studied rubbers. Changes in crosslinking density of butyl rubber compounds emphasize that degradation mechanism is strongly influenced by gamma-radiation doses higher than 50 kGy, since chain scission process predominates over crosslinking reaction.

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  • IPEN-DOC 23905

    ZAMBONI, CIBELE B. ; SILVA, DALTON G.N. da ; MORGADO, RAFAEL S. ; ZANATA, THIAGO; LEAL, LUIS G.M. ; MOREIRA, DAVISON de M.. Quantitative analysis of light elements using compact XRF spectrometers. In: RIO SYMPOSIUM ON ATOMIC SPECTROMETRY, 14th, 02-07 de abril, 2017, Vitória, ES. Abstract... 2017.

    Abstract: The use of analytical techniques to investigate specific ions in body fluids has increase in last year’s presenting significant progress in clinical tests. This motivated us to check the performance of two compacts XRF spectrometers (X-123SDD, Amptek) consisting of Ag and Au X-ray mini-tubes associated with a Si Drift detector (25 mm2 x 500 μm) with Be window (12.5 μm) for this clinical finality. Using the Energy Dispersive X-Ray Fluorescence technique (EDXRF) specific ions of clinical relevance, such as, Ca, Cl, Fe and K were investigated using standards (certified solutions). A simplified sample preparation procedure was proposed, i.e., the liquid samples were dripped in filter paper (Whatman, n° 41) and dried for a few seconds using infrared lamp and dry for few seconds using infrared lamp. The performance of these spectrometers were checked by evaluation of several parameter (linearity, reproducibility, accuracy, precision, sensitivity and detection limit) usually considered for validation procedures on analytical methods, in according to ISO 17025 and EURACHEM/CITAC norms. For simultaneous determination of these ions, the optimized excitation condition was established by 300s using 5μA and 30kV. The method was proved to give reliable results with limits of detection at levels of 0.23 to 0.58 mgL−1 for. Considering that the ranges of the body fluids, such as: blood, saliva, serum and urine, are in the order of hundreds of mg L-1, this procedure is very promising for ions dosage requiring a small amount of sample (50μL, 10 times less comparatively to the conventional tests), simultaneous analysis, short time of analysis (minutes) and simple sample preparation. In addition, this procedure offers a non-destructive alternative for clinical usage. Finally, these compact spectrometers have potential use when the biological material is scarce, case of the pediatric practice in newborns and premature infants (blood collection is the main cause of transfusions) and in vivo tests, in small size animal model (mice and rats), in order to evaluate the safety of new drugs, vaccines and others medical supplies.

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  • IPEN-DOC 23904

    SIMONS, SIMONE M.; LEAO, ANICARINE R.; WEN, FAN H.; ZAMBONI, CIBELE B. . Comparative study of cl determination in antilonomic serum for caterpillars lonomia obliqua walker using EDXRF. In: RIO SYMPOSIUM ON ATOMIC SPECTROMETRY, 14th, 02-07 de abril, 2017, Vitória, ES. Abstract... 2017.

    Abstract: The caterpillars Lonomia obliqua Walker (L. obliqua) species is very poisonous and has the ability to cause serious and fatal hemorrhagic effects in humans after contact [1]. The Butantan Institute (Research Center, São Paulo city) produces antilonomic serum (antidote), to reverse such effects, using caterpillars mainly from Paraná (PR) where the prevalence of incidents is high. However, in the last year, this species has present in various regions (Santa Catarina, São Paulo and Rio Grande do Sul) from Brazil and there is no data of its elemental composition to ensure that the antivenom produced (considering the different origins) may be used to manufacture serum unchanged in the final product. Recent measurements identified mainly Cl was majority [2]. In this investigation, we intend to check the Cl range of the antigen obtained from Parana (standardize the antigen) using EDXRF. The XRF measurements were performed using MINI X-ray spectrometer (PXRFS) from Amptek (Bedford, MA, USA) model X-123 SDD with Ag X-ray tube. The characteristics X-ray fluorescent intensity of Cl was measured with a Si detector (Si Drift 25 mm2 x 500 μm/ 0.5 mil. The excitation was performed using 30 kV and 5 μA excitation for a counting time of 100s. The quantitative analysis was performed using WinQxas software program (IAEA, version 1.3). A comparison using data from Neutron Activation Analysis emphasizes a good agreement, suggesting the viability of using the compact spectrometer to monitor the antigens from different origins in fast and efficient way. These data can be used to standardize a specific antilonomic serum for caterpillars (L. obliqua) coming from different regions of Brazil, based in Cl range, which reduce cost in the serum production process, meeting the standards of good manufacturing practices and good laboratory practice.

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  • IPEN-DOC 23901

    GIOVANNI, DALTON N. da S.; METAIRON, SABRINA ; ZAMBONI, CIBELE B. ; SUZUKI, MIRIAM F. ; BUENO JUNIOR, CARLOS R.; RIZZUTTO, MARCIA de A.. Iron determination in whole blood samples of dystrophic mice strains using X-ray fluorescence spectrometry. In: RIO SYMPOSIUM ON ATOMIC SPECTROMETRY, 14th, 02-07 de abril, 2017, Vitória, ES. Abstract... 2017.

    Abstract: The use of alternative analytical techniques to investigate specific electrolytes in body fluids has increased in past years with the goal of adding advantages and simplifications, compared to the procedures used in conventional clinical practice. Specifically, in this study, a portable X-ray spectrometer (PXRFS) from Amptek (model X-123 SDD) with Ag X-ray mini-tube has been employed for the whole blood iron measurements in mice species with Muscular Dystrophy (Dmdmdx/J, SJL/J and A/J). The term whole blood refers to solid (cells) and liquid (plasma) components in blood. Usually the conventional procedures for clinical tests are performed in serum using samples of 0.5 to 1.0 mL. However, when the biological material is scarce (case of small size animal model, i.e. mice), the possibility to use whole blood became a fascinating alternative for clinical practice: the Energy Dispersive X-Ray Fluorescence (EDFRX) analyses, using PXRFS, can be done using 50 μL (the total body blood in mice is around 1.0 mL). In this study, we investigated whole blood samples of dystrophic mice strains, a genetic disease that cannot be reversed. In this investigation, twenty samples were investigated. The blood samples were obtained from Jackson Laboratory (Maine, USA) and further inbred at IPEN. The whole blood was collected by the retro–orbital venous plexus and immediately transferred to filter paper (Whatman n41), prior to its coagulation. No anticoagulants or reagents have been used to prevent interference on the results. This procedure simplify the preparation and conservation of biological samples: it is not a destructive procedure and the sample does not need refrigeration. The collection was performing according to the rules approved by Animal Research Ethics Committee (087/99). A comparative study between EDXFX and INAA (Instrumental Neutron Activation Analysis) data was also carried out and the results are in good agreement. This alternative procedure was capable, in a few minutes, to determine whole blood Fe concentrations higher than 6.5 mgL-1, without chemical digestion using direct and non-destructive analysis. The study of Fe behavior in whole blood samples of mice with muscular dystrophy contributes to its diagnosis.

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  • IPEN-DOC 23894

    BARROS, LIVIA F. ; PECEQUILO, BRIGITTE R.S. . The influence of environmental aspects in the variation of natural radioactivity levels in selected brazilian beach sand samples. In: CESSA, MARGARET (Ed.). Beaches: erosion, management practices and environmental implications. Hauppauge, Nova Iorque, EUA: Nova Science Publishers, 2014. p. 73-99.

    Abstract: At beaches, environmental implications added to shoreline erosion and artificial interventions on native structure may alter the concentration of the natural radionuclides in certain regions of the coast. In this work, eleven locations at Camburi beach, known to be a naturally high background region, were studied in 2011. The influence of environmental aspects, as geology, average temperatures, variations of the height of the tides and the rainfall levels were related with the variation of 226Ra, 232Th and 40K activities concentrations, during the year 2011 and annual effective dose were evaluated from the 226Ra, 232Th and 40K sands activities concentrations. Sand samples were monthly collected during 2011, dried, sealed in standard 100 mL HPDE polyethylene flasks and measured by high resolution gamma spectrometry after a 4 weeks in-growth period. The results, considering samples gamma-rays self-attenuation, show activities concentrations in the range from 4 Bq kg-1 to 1380 Bq kg-1 for 226Ra, 9 Bq kg-1 to 7453 Bq kg-1 for 232Th, and 6 Bq kg-1 to 504 Bq kg-1 for 40K. The absorbed dose rate (D) in outdoor air values laid from 9 nGy.h-1 to 5160 nGy.h-1 .The annual effective dose values for the sand samples considering a daily exposure of 24 hours all year round laid from 0.05 mSv.y-1 to 31.64 mSv.y-1. The annual effective dose values for the sand samples considering daily exposure of one hour all year round laid from 0.002 mSv.y-1 to 1.318 mSv.y-1.The annual effective dose (E) for the beach collection points for a resident of Vitoria city, with one hour daily exposure all year round by collection point laid from 0.003 mSv.y-1 to 0.232 mSv.y-1. The annual effective dose (E) for the collection points from Camburi beach for a tourist with 8 hours daily exposure on january, july or both, laid from 0.004 mSv.y-1 to 0.446 mSv.y-1. For a resident of Vitoria city and for a tourist, in the conditions of exposure of this work, all values of E are lower than the limit of 1 mSv y-1, recommended for ICRP 60 for the general public. In some months of the year 2011, low average temperatures, low rainfall levels and low variations of the height of the tides can be related with high 226Ra, 232Th and 40K activities concentrations. In April and in October, high rainfall levels and high variations of the height of the tides can be related with low 226Ra, 232Th and 40K activities concentrations.

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  • IPEN-DOC 23898

    MARTINI, G.D.; ROGERO, S.O. ; AZEVEDO, J.D.; ROGERO, J.R. . Evaluation of DEET cytotoxicity on Perna perna mussels. In: ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th, May 11-15, 2014, Basel, Switzerland. Abstract... 2014. p. 164-164.

    Abstract: Recent studies have identified the presence of several emerging pollutants in aquatic environments. The occurrence in different environmental matrices has been continuously reported, highlighting the need for toxicity studies. Developed in the 1940s and present in many commercially available formulations, the DEET (N,N-diethyl-meta-toluamide) is the active agent used in most insect repellents. Several studies have been identified the DEET presence in surface water and in wastewater treatment plant (WWTP) effluent, so this compound is considered an emerging pollutant and studies indicate that DEET is only slightly toxic to aquatic organisms. Even nowadays the environmental destiny of DEET is not completely understood. Although DEET is considered a compound resistant to degradation and commonly found in surface water, there are not complete assessments for ecological risk of DEET, including studies evaluating chronic toxicity to aquatic organisms. In this study was evaluated the DEET toxicity and the effects caused in lysosomes of Perna perna mussels hemocytes. For this purpose, firstly was performed the acute toxicity assay to identify the DEET concentration that causes 50% mortality of exposed organisms to DEET concentrations in the range of 75 to 400 mg L-1 and the obtained lethal dose (LC50) was 114.27 mg L-1. To assess the stability of the lysosomal membrane in organisms exposed to this compound was carried out the cytotoxicity assay utilizing DEET concentrations in a range of 0.001 to 1.0 mg L-1. In fact, is important reinforce that the data of DEET cytotoxicity showed disturbances in Perna perna mussels in all tested concentrations.

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  • IPEN-DOC 23893

    AZEVEDO, J.D.; SILBIGER, H.N.; SARKIS, J.E. . Hepatic histopathological findings in Ariidae Cathorops spixii submitted to anthropogenic metal exposure in two Brazilian estuaries. In: ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th, May 11-15, 2014, Basel, Switzerland. Abstract... 2014. p. 150-150.

    Abstract: Histopathological lesions are used as biomarkers of contamination by organic and inorganic chemical compounds in the aquatic systems and can reflect chronic effects in sentinel species. Previous work demonstrated the ability of the catfish Cathorops spixii to be an efficient bioindicator species for metals contamination. Cananéia estuary is considered an area of environmental protection, and UNESCO World Heritage Site (WHS), and as such, several biomonitoring studies in the São Paulo State, Brazil, use this estuary as a reference environment due to the fact that it shows very little human influence. However, in recent years, several studies have been indicating the presence of some toxic metals, for instance mercury (Hg) and lead (Pb). Santos-São Vicente estuary has a long history of contamination and release of pollutants, for instance metals such as Pb, Cd, Ni, Mn, Cu and Hg as a consequence of different anthropogenic activities such as petrochemical, metallurgical and harbor. In the present work, histopathological lesions in liver were investigated in the Ariidae Cathorops spixii from Cananéia estuary and in two sites affected by industrial and domestic sewage disposal, in order to verify the possible impact of the introduction of metals to the local fish species. Fish were collected in Cananéia and in two sites of the Santos São-Vicente estuary subjected to different anthropogenic influence (industrial and domestic sewage disposal). Biometric data (total length –TL- and total weight -TW- of each fish) was obtained and the fish dissected by collection of the hepatic liver to histopathological and metals analysis. Analyses of Hg was performed by FIA-CV AAS and Pb, Cd, Cu, Mn and Ni concentrations were determined by ICP MS. Fish from Cananéia showed higher levels of Mn (80%) and Cu (66%). The most important injuries observed in individuals from the impacted areas (industrial and domestic sewage) were the presence of necrotic areas, hepatitis focal, vacuolization and rupture of blood vessels. The occurrences of lesions were significantly higher in individuals from domestic and industrial area than in fish from Cananéia estuary (p>0.05).

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  • IPEN-DOC 23892

    AZEVEDO, J.D.; SARKIS, J.E. ; HORTELLANI, M.A. . Hepatic bioavailability of Total Mercury (THg) in Cichla sp from Tapajós River Region, Brazilian Amazon. In: ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th, May 11-15, 2014, Basel, Switzerland. Abstract... 2014. p. 360-361.

    Abstract: Most of the Hg occurring in the Amazon is of natural origin. However, the intense gold mining activity in the region has increased the levels of this toxic metal, especially in the middle section of the Tapajós River. Specimens of the Cichla genus have great economic and ecological significance, as they are the most consumed fish in the Brazilian Amazon region and are at the top of the Amazon food chain. In addition, they are a good indicator of Hg bioaccumulation because the concentrations of Hg in their tissues tend to reflect the accumulation process of successive exposure. A total of 26 adult specimens of Cichla sp were obtained by fisheries in the Tapajós River region’s contaminated gold mining area. In the laboratory, morphometric data of each fish was taken, for instance total length, total weight and sex. The liver tissue was removed from each specimen, washed with distilled water, packed in polyethylene identified bags and kept at - 20ºC for subsequent total mercury (THg) analysis. Assays were carry out in the total liver and in the cytosol hepatic fraction obtained by ultracentrifugation of the tissue in buffer Tris-HCl 10 mM, pH 7.4. The analyses were performed by flow injection Cold Vapor Atomic Absorption Spectrometry (FIA-CV AAS) with detection (DL) and quantification limits (QL) of 0.527 and 2.585 μg Kg-1, respectively. Individuals of C. sp had active gonadal maturation, including spawning and post-spawning individuals. The average length and total weight was 395 ± 51 mm and 840 ± 418 g, respectively. High levels of THg were obtained in the total liver ranging from 1.7 mg Kg-1 to 3.8 mg Kg-1. However, only 0.2% of the THg were available to the cytosol hepatic fraction. A significant and positive correlation (rs = 0.65) was found between the concentration of the THg in the total tissue and in the hepatic cytosol fraction. These data are a preliminary study with respect to the bioavailability of total mercury (THg) in different compartments of the hepatic tissue of C. sp in order to understand some aspects of the detoxification mechanism of this toxic metal in bioindicator species from the Amazon region. In fact, it was possible to observe differences in the transference of this important toxic metal into the cell and its linkage in the compartments.

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  • IPEN-DOC 23891

    AZEVEDO, J.D.; SARKIS, J.E. ; ROGERO, S.O. . Evaluation of the detoxification mechanisms of metals in aquatic organisms by characterization of hepatic metallothionein (MT). In: ANNUAL MEETING OF THE SOCIETY OF ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24th, May 11-15, 2014, Basel, Switzerland. Abstract... 2014. p. 151-151.

    Abstract: Metallothionein (MT) has been thoroughly used as a biomarker of metals exposure. MT is a low-molecular-weight protein which has many sulfhydryl groups due to the large amount of cysteine in the molecule. These sulfhydryl groups bind a variety of metals and therefore, presumably, make them less toxic to other cellular constituents. However, biochemistry aspects of the protein as isoforms identification and quantification and their specific mechanisms of detoxification in sentinel species are, as yet, weak. Previous data showed that fish, such as the catfish Cathrorops spixil, are efficient bioindicator species to metals contamination in coastal aquatic areas under anthropogenic influence, for instance inputs of Pb, Cd, Hg, Ni, Fe, Zn, Cu and Mn. C. spixil is the most common catfish in the Brazilian coast and has a feeding habit mainly of materials and organisms upon the sediment, where the availability of contaminants is high. In order to understand intrinsic aspects of the detoxification process of toxic metals in sentinel species in natural and anthropogenic conditions, hepatic samples of C. spixii were collected in a non-polluted (Cananeia), as well as one polluted, estuary (Santos-São Vicente) and tested under different assays in order to establish an effective bioanalytical technique to purify the protein like-MT, identify and quantify the specific isoforms and the metal contents in the cell. Therefore, hepatic samples were submitted to ultracentrifugation, thermocoagulation and a chromatographic purification and identification of MT isoforms by size-exclusion-HPLC and anion-exchange-HPLC, respectively. The elution of the protein was made with online UV/Vis detection. Metals in hepatic cytosols were also quantified by HR-ICP-MS. Obtained results showed that the established protocol of bioanalytical technique was effective to purify MT-like protein by SE-HPLC and to identify its isoforms by AE-HPLC. With these analytical strategies, it was possible to identify two kinds of MT isoforms (MT-1 and MT-2) in the C. spixil. Fish MT-1 from polluted areas showed a strong linkage with the levels of Cu, Hg, Pb, Fe and Ni suggesting that the MT-1 is an effective biomarker of metal contamination.

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  • IPEN-DOC 23889

    VASCONCELLOS, MARINA B.A. ; ISHIKAWA, N.M.; PAIVA, M.J.T.R. de; LOMBARDI, J.V.; MOREIRA, E.G. . Study of methylmercury biomagnificationww in wistar rats (Rattus novergicus) fed with methylmercury contaminated Tilapia (Oreochromis niloticus). In: ANNUAL INTERNATIONAL SYMPOSIUM ON ENVIRONMENT, 8th, May 13-16, 2013, Athens, Greece. Abstract... 2013. p. 55-55.

    Abstract: In the present paper, the biomagnification of methylmercury was studied in Wistar rats (Rattus norvergicus) fed with tilapia fish (Oreochromics niloticus) that were fed with diet contaminated with methylmercury. Wistar rats have several characteristics common to mammals in general and are frequently used in biomedical studies of physiological and morphological nature. Two groups of fish were fed with contaminated diet (Hg concentration 1= 1.03 ± 0.15 μg g-1 and Hg concentration 2 = 8.27 ± 1.25 μg g-1) and one group was fed with diet not contaminated with methylmercury (control group). The experiment was conducted for a period of forty two days. Afterwards, two groups of Wistar rats were fed, via gavage, with tilapia fish muscle diets, contaminated with methylmercury from the previous experiment (Hg concentration 1 = 0.31 ± 0.03 μg g -1 and Hg concentration 2 = 3.04 ± 0.94 μg g -1) for twenty eight days. Another group was fed in the same way with fish muscle diet from the controls of the tilapia experiment. The rats were fed with one gram of crushed and homogenized fish feed and afterwards they were fed ad libitum with their usual pelletized feed. In the rats, the blood and biochemical parameters as well as somatic relations were analyzed periodically and did not present significant differences between the evaluated groups. In the other hand, the rats that were fed with fish muscle containing the highest level of added methylmercury presented higher bioaccumulation of Hg in the kidney, liver, spleen, muscle and brain if compared to the group with lower added Hg. Methylmercury was determined as total Hg by CV AAS. The results showed the following comparative degree of accumulation of Hg: kidney > liver > spleen > muscle = brain.

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  • IPEN-DOC 23896

    JORGE, ANA E.S.; CAMPOS, CAROLINA P.; FREITAS, ANDERSON Z. de ; BAGNATO, VANDERLEI S.. Photodynamic therapy induces epidermal thickening in hairless mice skin: an optical coherence tomography assessment. In: IZATT, JOSEPH A. (Ed.); FUJIMOTO, JAMES G. (Ed.); TUCHIN, VALERY V. (Ed.) OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE DOMAIN OPTICAL METHODS IN BIOMEDICINE, 18th, February 01, 2014, San Francisco, CA, USA. Proceedings... Bellingham, WA, USA: International Society for Optics and Photonics, 2014. p. 89343N-1 - 89343-3. (SPIE Proceedings Series, 8934).

    Abstract: Photodynamic therapy (PDT) promotes skin improvement according to many practitioners, however the immediately in vivo assessment of its response remains clinically inaccessible. As a non-invasive modality, optical coherence tomography (OCT) has been shown a feasible optical diagnostic technique that provides images in real time, avoiding tissue biopsies. For this reason, our investigation focused on evaluates the PDT effect on a rodent model by means of OCT. Therefore, a normal hairless mouse skin has undergone a single-session PDT, which was performed with topical 5- aminolevulinic acid (ALA) cream using a red (630 nm) light emitting diode (LED) which reached the light dose of 75 J/cm2. As the optical imaging tool, an OCT (930 nm) with axial resolution of 6.0 microns in air was used, generating images with contact to the mouse skin before, immediately after, 24 hours, and 2 weeks after the correspondent procedure. Our result demonstrates that, within 24 hours after ALA-PDT, the mouse skin from the PDT group has shown epidermal thickness (ET), which has substantially increased after 2 weeks from the treatment day. Moreover, the skin surface has become evener after ALA-PDT. Concluding, this investigation demonstrates that the OCT is a feasible and reliable technique that allows real-time cross-sectional imaging of skin, which can quantify an outcome and predict whether the PDT reaches its goal.

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  • IPEN-DOC 23888

    SOUSA, MARCELO V.P.; PRATES, RENATO ; KATO, ILKA T. ; SABINO, CAETANO P. ; YOSHIMURA, TANIA M. ; SUZUKI, LUIS C. ; MAGALHAES, ANA C.; YOSHIMURA, ELISABETH M.; RIBEIRO, MARTHA S. . Inhomogeneity in optical properties of rat brain: a study for LLLT dosimetry. In: HAMBLIN, MICHAEL R. (Ed.); ARANY, PRAVEEN R. (Ed.); CARROLL, JAMES D. (Ed.) MECHANISMS FOR LOW-LIGHT THERAPY, 8th, February 02, 2013, San Francisco, CA, USA. Proceedings... Bellingham, WA, USA: International Society for Optics and Photonics, 2013. p. 856905-1 - 856905-6. (SPIE Proceedings Series, 8569).

    Abstract: Over the last few years, low-level light therapy (LLLT) has shown an incredible suitability for a wide range of applications for central nervous system (CNS) related diseases. In this therapeutic modality light dosimetry is extremely critical so the study of light propagation through the CNS organs is of great importance. To better understand how light intensity is delivered to the most relevant neural sites we evaluated optical transmission through slices of rat brain point by point. We experimented red (λ = 660 nm) and near infrared (λ = 808 nm) diode laser light analyzing the light penetration and distribution in the whole brain. A fresh Wistar rat (Rattus novergicus) brain was cut in sagittal slices and illuminated with a broad light beam. A high-resolution digital camera was employed to acquire data of transmitted light. Spatial profiles of the light transmitted through the sample were obtained from the images. Peaks and valleys in the profiles show sites where light was less or more attenuated. The peak intensities provide information about total attenuation and the peak widths are correlated to the scattering coefficient at that individual portion of the sample. The outcomes of this study provide remarkable information for LLLT dose-dependent studies involving CNS and highlight the importance of LLLT dosimetry in CNS organs for large range of applications in animal and human diseases.

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  • IPEN-DOC 23890

    XAVIER, CESAR C. ; MOTTA, CLAUDIO C.. A DC design of an X-band traveling-wave tube. In: INTERNATIONAL MICROWAVE AND OPTOELECTRONICS CONFERENCE, August 04-07, 2013, Rio de Janeiro, RJ. Proceedings... Piscataway, NJ: IEEE, 2013.

    Abstract: In this paper a dc design procedure of a X-band traveling-wave tube is described. This procedure consists in determine the geometry of the electron gun, the periodic permanent magnetic and the electron collector. It was found that an electron gun with a 0.26 μPerv, 9.1 kV, 225 mA and 0.6 mm beam radius without electrostatic beam control was suitable for this design. The PPM, constructed with samarium-cobalt magnetics shown a good agreement with theoretical design. Finally a three stage depressed collector, to recover part of nonused electron energy and improve the overall electronic efficiency of TWT, is presented. The simulations were run with Particle-incell Studio.

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  • IPEN-DOC 23887

    SILVA, ROBSON K.B. e ; MOTTA, CLAUDIO C.. Design and simulation of a 1.3 GHz input klystron cavity. In: INTERNATIONAL MICROWAVE AND OPTOELECTRONICS CONFERENCE, August 04-07, 2013, Rio de Janeiro, RJ. Proceedings... Piscataway, NJ: IEEE, 2013.

    Abstract: This paper presents a sequence of steps to begin the project of a cylindrical reentrant klystron cavity for 1.3 GHz. One analytical method and different CAD tools such as SUPERFISH and 3D eigenmode solver have been used for designing the cavity. The external coupling problem of the cavity is also discussed and is proposed a technique to get the critical coupling.

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  • IPEN-DOC 23886

    SENE, FRANK F. ; SANTOS, VINICIUS O.; MOTTA, CLAUDIO C.. Synthesis and characterization of Ni-Mo sintered alloy foils for microwave tube manufacturing. In: INTERNATIONAL MICROWAVE AND OPTOELECTRONICS CONFERENCE, August 04-07, 2013, Rio de Janeiro, RJ. Proceedings... Piscataway, NJ: IEEE, 2013.

    Abstract: The Ni-Mo composite alloy, to be used in microwave vacuum tube manufacturing, was prepared from metal powders previously homogenized, pressed and sintered at 1200oC by induction heating. The Ni-Mo sintered discs were annealed at 1150oC for 20min, and submitted to the roller process in 5 cycles to obtain a thin foil with 0.10 mm of thickness, for brazing process adopted to join the cathode components. The samples were submitted to scanning electron microscopy (SEM) coupled to energy dispersive spectroscopy analysis (EDS) to characterize the composition and microstructure. X-ray diffraction (XRD) was performed to determine the crystalline structure and energy dispersive X-ray fluorescence spectroscopy (EDX) was performed to determine the chemical composition and the homogeneity of the composite alloy.

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  • IPEN-DOC 23883

    PRETTO, A. ; IZIDORO, J.C. ; GATTI, L.V. ; CORREIA, C.S.C. ; MARTINEWSKI, A. ; BASSO, L.S. ; BORGES, V.F. ; ROSSATI, C.; MILLER, J.B.; CROTWELL, A.; TANS, P.. Analysis of the influence of co2 concentration and others external factors on the N2O quantification. In: MEETING ON CARBON DIOXIDE, OTHER GREENHOUSE GASES AND RELATED TRACERS MEASUREMENT TECHNIQUES, 17th, June 10-13, 2013, Beijing, China. Proceedings... 2013.

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  • IPEN-DOC 23882

    POLITANO, R. . Nitriding modelling in nanoscale. In: INTERNATIONAL CONFERENCE ON MATHEMATICAL MODELLING IN PHYSICAL SCIENCES, 1st, September 3-7, 2012, Budapest, Hungary. Proceedings... Bristol, UK: IOP Publishing, 2013. p. 1-8.

    Abstract: Modelling on Plasma and gas nitriding of austenitic and low alloy steels have strong influence of interfacial - nanoescale phenomena. Gamma prime and epsilon nitrogen-iron phases evolution during the nitriding process wasn't found explicitly simulated on literature. On present work we simulated nitrind process on low alloy steels - with their precipitation phenomena and nitrides moving interfaces - using diffusional models plus cellular automata. Surface effects and heterogeneities and local transition phenomena considered on our models show that some experimental results considered "errors" on literature are predicted by our simulations. In present work parameters like diffusion coefficient and surface conditions and gradient of nitrogen concentrations between different phases was measured.

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  • IPEN-DOC 23873

    IMAM, MUHAMMAD A.; GOMES, M.G.; MOURA, ESPERIDIANA A.B. ; JEELANI, SHAIK; RANGARI, VIJAY K.. Effect of electron-beam irradiation on Nylon-6/diamond coated CNTS composite fiber. In: SAMPE Tech, October 21-24, 2013, Wichita, KS, USA. Proceedings... 2013.

    Abstract: Polyamides (Nylon-6) are engineering plastic with excellent properties which are useful in several industrial applications and are valued for their high strength and processability. The addition of filler such as diamond coated Carbon Nano Tubes (CNTs) to form molded composites has increased the range of polyamide applications due to the resulting increase in strength. The effects of electron-beam irradiation on these thermoplastic nanocomposites are either increases the cross-linking or causes chain scission. In this study, DN-coated CNTs were synthesized using sonochemical technique in the presence of cationic surfactant CTAB. These hybrid nanoparticles were characterized using TEM analysis. The nanoparticles were then introduced into Nylon-6 polymer through a melt extrusion process to form nanocomposite fibers that were tested for their mechanical & thermal properties (e.g. tensile and Differential Scanning Calorimetry). These composites were further exposed to the electron-beam (160 KGy, 132 KGy and 99 KGy) using a 1.5 MeV electron beam accelerator, at room temperature, in presence of air and tested for their thermal and mechanical properties. The ultimate tensile strength were found to be 521 MPa, 690 MPa and 425 MPa for radiated (99 KGy,132 KGy and 160 KGy) DN/CNTs/Nylon-6 single fibers as compared to 346 MPa for DN/CNTs/Nylon-6 single fibers without irradiation and 240 MPa for neat Nylon-6 single fibers respectively. Differential Scanning Calorimetry (DSC) analysis results were showed that degree of cure was increased because of cross-linking which was expected at high electron-beam radiation dose.

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  • IPEN-DOC 23868

    CARDENAS, JOSE P.N. ; MADI FILHO, TUFIC ; PETRI, ANNA R. ; SANTOS, ROBINSON A. dos ; MARTINS, JOAO F.T. ; CARVALHO, DIEGO V.S.; ALVARENGA, T.; BELLEZZO, M.; LARANJO, G.; LIMA, M.; OLIVEIRA, P.; PEREIRA, MARIA da C.C. . Experimental and MCNP studies of paraffin and polyethylene in neutron moderation and BF3 detector efficiency. In: INTERNATIONAL CONFERENCE ON ADVANCEMENTS IN NUCLEAR INSTRUMENTATION, MEASUREMENT METHODS AND THEIR APPLICATIONS, 3rd, June 23-27, 2013, Marseille, France. Proceedings... Piscataway, NJ: IEEE, 2013.

    Abstract: The Nuclear and Energy Research Institute – IPEN, offers post-graduate programs, namely: Nuclear Technology - Applications (TNA), Nuclear Technology - Materials (TNM), Nuclear Technology - Reactors (TNR). The Institute programs mission is to form expert technicians and engineers with a strong knowledge in their discipline to work in the nuclear area. The course: “Theoretical Fundaments and Practices of the Instrumentation used in Nuclear Data Acquisition” covers the use of laboratory nuclear instrumentation and the accomplishment of experiments to obtain nuclear parameters. One of these experiments is object of this work: “Experimental and MCNP Studies of Paraffin and Polyethylene Neutron Moderation and BF3 Detector Efficiency”. Neutrons are uncharged particles and, therefore, cannot be detected by Coulomb interactions. Thus, the detector assembly used must contain some kind of material with high cross section for interaction with neutrons, called converters. A boron trifluoride (BF3) detector was used in this experiment to detect neutron in real time. However, the response of this arrangement varies according to the energy range of incident neutrons. Their efficiency for thermal neutrons is above 90%, but, this result decreases, significantly, for neutrons of energy greater than 0.5 eV. The neutron moderation and, consequently, its energy variation were obtained by interposing different thicknesses of moderator material (Paraffin or Polyethylene) between the source and the detector.

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  • IPEN-DOC 23876

    MATTOS, LUIS A.T. de ; MADI FILHO, TUFIC ; MELDONIAN, NELSON L. . Methodological proposal for identification and evaluation of environmental aspects and impacts of IPEN nuclear facilities: a case study applied to the nuclear fuel center. In: INTERNATIONAL CONFERENCE ON ADVANCEMENTS IN NUCLEAR INSTRUMENTATION, MEASUREMENT METHODS AND THEIR APPLICATIONS, 3rd, June 23-27, 2013, Marseille, France. Proceedings... Piscataway, NJ: IEEE, 2013.

    Abstract: This work presents an application of Failure Mode Effect Analysis (FMEA) to the process of identification of environmental aspects and impacts as a part of implementation and maintenance of an Environmental Management System (EMS) in accordance with the ISO 14001 standard. Also, it can contribute, as a complement, to the evaluation and improvement of safety of the installation focused. The study was applied to the Nuclear Fuel Center (CCN) of Nuclear and Energy Research Institute (IPEN) , situated at the Campus of University of Sao Paulo, Brazil. The CCN facility has the objective of promoting scientific research and of producing nuclear fuel elements for the IEA-R1 Research Reactor. To identify the environmental aspects of the facility activities, products, and services, a systematic data collection was carried out by means of personal interviews, documents, reports and operation data records consulting. Furthermore, the processes and their interactions, failure modes, besides their causes and effects to the environment, were identified. As a result of a careful evaluation of these causes it was possible to identify and to classify the major potential environmental impacts, in order to set up and put in practice an Environmental Control Plan for the installation under study. The results have demonstrated the validity of the FMEA application to nuclear facility processes, identifying environmental aspects and impacts, whose controls are critical to achieve compliance with the environmental requirements of the Integrated Management System of IPEN. It was demonstrated that the methodology used in this work is a powerful management tool for resolving issues related to the conformity with applicable regulatory and legal requirements of the Brazilian Nuclear Energy Commission (CNEN) and the Brazilian Institute of Environment (IBAMA).

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  • IPEN-DOC 23875

    MADI FILHO, TUFIC ; CARDENAS, JOSE P.N. ; PETRI, ANNA R. ; SANTOS, ROBINSON A. dos ; MARTINS, JOAO F.T. ; CARVALHO, DIEGO V.S.; ALVARENGA, T.; BELLEZZO, M.; LARANJO, G.; LIMA, M.; OLIVEIRA, P.; PEREIRA, MARIA da C.C. . Am-Be neutron irradiator used for nuclear instrumentation training. In: INTERNATIONAL CONFERENCE ON ADVANCEMENTS IN NUCLEAR INSTRUMENTATION, MEASUREMENT METHODS AND THEIR APPLICATIONS, 3rd, June 23-27, 2013, Marseille, France. Proceedings... Piscataway, NJ: IEEE, 2013.

    Abstract: A neutron irradiator was assembled at IPEN (Nuclear and Energy Research Institute) facility to perform qualitative-quantitative analysis of materials, using thermal and fast neutrons. In order to determine the 116mIn decay constant, a thermal flux obtained experimentally by Monte Carlo N-Particle Transport Code-MCNP, in a previous work, was used in the nuclear experiment. The activity calculated from the activation parameters was 13.51±0.17 kBq and the activity determined experimentally was 12.51± 0.36 kBq. The decay constant determined by the pulse height analyzer (PHA) measures was 211.4 μ·s-1, and that determined by fitting the data using a Multichannel Scaler (MCS) system was 200.3 ± 1.6 μ·s-1. The half-life of 116mIn found in the literature is 3256.8 s, which corresponds to a decay constant of 212.8 μ·s-1. The present experiment does not intend to establish a new value for the decay constant: it solely aims students’ practical exercises in nuclear properties of elements. This experiment is part of the nuclear experimental course.

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  • IPEN-DOC 23867

    CARDENAS, JOSE P.N. ; MADI FILHO, TUFIC ; RICCI FILHO, WALTER ; CARVALHO, MARCOS R. de ; BENEVENUTI, ERION de L. ; GOMES NETO, JOSE . Digital systems implemented at the IPEN nuclear research reactor (IEA-R1): results and necessities. In: INTERNATIONAL CONFERENCE ON ADVANCEMENTS IN NUCLEAR INSTRUMENTATION, MEASUREMENT METHODS AND THEIR APPLICATIONS, 3rd, June 23-27, 2013, Marseille, France. Proceedings... Piscataway, NJ: IEEE, 2013.

    Abstract: IPEN (Nuclear and Energy Research Institute) was founded in 1956 with the main purpose of doing research and development in the field of nuclear energy and its applications. It is located at the campus of University of Sao Paulo (USP), in the city of Sao Paulo, in an area of nearly 500,000 m2. It has over 1.000 employees and 40% of them have qualification at master or doctor level The institute is recognized as a national leader institution in research and development (R&D) in the areas of radiopharmaceuticals, industrial applications of radiation, basic nuclear research, nuclear reactor operation and nuclear applications, materials science and technology, laser technology and applications. Along with the R&D, it has a strong educational activity, having a graduate program in Nuclear Technology, in association with the University of Sao Paulo, ranked as the best university in the country. The Federal Government Evaluation institution CAPES, granted to this course grade 6, considering it a program of Excellence. This program started at 1976 and has awarded 458 Ph.D. degrees and 937 master degrees since them. The actual graduate enrollment is around 400 students. One of major nuclear installation at IPEN is the IEA-R1 research reactor; it is the only Brazilian research reactor with substantial power level suitable for its utilization in researches concerning physics, chemistry, biology and engineering as well as for producing some useful radioisotopes for medical and other applications. IEA-R1 reactor is a swimming pool type reactor moderated and cooled by light water and uses graphite and beryllium as reflectors. The first criticality was achieved on September 16, 1957. The reactor is currently operating at 4.5 MW power level with an operational schedule of continuous 64 hours a week. In 1996 a Modernization Program was started to establish recommendations in order to mitigate equipment and structures ageing effects in the reactor components, detect and evaluate obsolescence of some electrical and electronic systems. In this work we will show a retrospective and results of digital systems applied to IEA-R1 reactor concerning electronic equipments and systems refurbishment and modernization and the necessity of a new control console implementation.

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  • IPEN-DOC 23866

    BORGES, R.; SILVA, A.C. da ; MARCHI, J.. Caracterização de biovidros bioabsorvíveis para aplicação em medicina regenerativa. In: CONGRESSO BRASILEIRO DE CERAMICA, 57.; CONGRESSO IBEROAMERICANO DE CERAMICA, 5., 19-22 de maio, 2013, Natal, RN. Anais... São Paulo: ABCERAM, 2013.

    Abstract: Neste trabalho, foram estudadas três composições de vidros bioaborvíveis com potencial aplicação em medicina regenerativa. Vidros no sistema SiO2 - (37- x)Na2O- (5+x)CaO com 6% de P2O5 (x = 0, 5 e 10) foram obtidos por fusão seguida de superresfriamento e posterior recozimento. Sua biocompatibilidade foi avaliada por imersão em solução SBF e caracterização da superfície dos vidros após diferentes períodos de imersão. Os vidros foram caracterizados antes e após o ensaio de bioatividade pelas técnicas de difração de raio-x, espectroscopia de infravermelho na transformada de Fourier e microscopia eletrônica de varredura e por estudo de variação de massa. Os resultados obtidos indicam que nestes vidros bioabsorvíveis o mecanismo de bioatividade ocorre, primeiramente, através da formação de uma camada de silicato de cálcio que atua como nucleador de deposição de posterior segunda camada de hidroxiapatita. Também foi verificado que a cinética de nucleação de hidroxiapatita pode ser controlada através da razão Ca/Na.

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  • IPEN-DOC 23895

    CHOQUECHAMBI CATORCENO, LITZY L.; LIMA, NELSON B. de . Influence of rolling direction and temperature on the texture formation in rolled AZ31B magnesium alloy. In: ALDERMAN, MARTYN (Ed.); MANUEL, MICHELE V. (Ed.); HORT, NORBERT (Ed.); NEELAMEGGHAM, NEALE R. (Ed.) MAGNESIUM TECHNOLOGY; TMS ANNUAL MEETING & EXIBITION, February 16-20, 2014, San Diego, CA, USA. Proceedings... Hoboken, NJ, USA: John Wiley & Sons, Inc., 2014. p. 245-250.

    Abstract: In this study, effects of rolling direction and temperature on the texture formation were investigated for the purpose of enhancing formability of rolled AZ31B alloy. For this, specimens were cold and warm rolled from a rolled sheet, both parallel and perpendicular to the rolling direction and textures of the sheets processed were examined. The results showed that samples rolled parallel to the rolling direction generated strong basal texture during cold and warm rolling process. However, on sample rolled perpendicular to the rolling direction at 300°C resulted in the formation of basal textures with low intensities. The (0002) pole was inclined towards the transverse direction. The occurrence of recrystallization at high temperatures contributed to the formation of low intensities basal textures.

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  • IPEN-DOC 23863

    SARTORI, MARIANA N. ; OLIVEIRA, RENE R. ; DIAZ, FRANCISCO R.V.; ORTIZ, ANGEL V. ; RANGARI, VIJAYA K.; MOURA, ESPERIDIANA A.B. . Preparation and characterization of PBT/clay nanocomposite. In: WANG, SHIJIE (Ed.); DULRIZAC, JOHN E. (Ed.); FREE, MICHAEL L. (Ed.); HWANG, JAMES Y. (Ed.); KIM, DANIEL (Ed.) T.T. CHEN HONORARY SYMPOSIUM ON HYDROMETALLURGY, ELECTROMETALLURGY AND MATERIALS CHARACTERIZATION; TMS ANNUAL MEETING & EXIBITION, March 11-15, 2012, Orlando, Florida, USA. Proceedings... Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. p. 731-739.

    Abstract: This work presents the preparation and characterization of a nanocomposite based on Poly(butylene terephthalate) PBT and Brazilian smectitic clay (bentonite chocolate clay). Before being incorporated as clay nanoparticles in PBT resin, the clay was organically modified by the addition of a quaternary salt and sodium carbonate. PBT/Clay nanocomposite (96.3:3.70 wt %) was obtained by using a twin-screw extruder machine. After extrusion process, the nanocomposite was characterized by tensile, flexural and impact tests, SEM, Vicat, HDT, DSC, TGA and XRD tests. The results showed that the properties of the nanocomposite obtained were superior to those of neat PBT. Concerning the temperature of thermal distortion (HDT) an expressive gain of around 45 % was presented to PBT/Clay nanocomposite compared to PBT evidencing the interaction of nanofiller with the polymeric matrix.

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  • IPEN-DOC 23881

    ORTIZ, ANGEL ; ESCANHOELA, CORDELIA M.F. ; GOMES, MICHELLE ; OLIVEIRA, RENE R. ; DIAZ, FRANCISCO R.V.; MOURA, ESPERIDIANA A.B. . Comparison between HDPE/clay and HDPE/piassava fiber/clay treated by electron beam radiation. In: HWANG, JIANN-YANG (Ed.); BAI, CHENGGUANG (Ed.); CARPENTER, JOHN (Ed.); IKHMAYIES, SHADIA J. (Ed.); LI, BOWEN (Ed.); MONTEIRO, SERGIO N. (Ed.); PENG, ZHIWEI (Ed.); ZHANG, MINGMING (Ed.) CHARACTERIZATION OF MINERALS, METALS, AND MATERIALS. 142th; ANNUAL MEETING & EXIBITION, March 3-7, 2013, San Antonio, Texas, USA. Proceedings... Hoboken, NJ, USA: John Wiley & Sons, Inc., 2013. p. 425-432.

    Abstract: This work evaluates changes in morphological, mechanical and thermo-mechanical properties of electron-beam irradiated HDPE/nanoclay and HDPE/Piassava fiber/nanoclay. Material samples irradiated with 100 and 200 kGy using a 1.5 MeV electron beam accelerator (room temperature, presence of air) were submitted to tensile, flexural, and HDT tests. MFI, SEM and XRD analyses were carried out. Correlation between properties was discussed. Results showed that incorporation of piassava (Attalea funifera Mart) fiber and nanoclay, followed by irradiation represented significant gain in thermo-mechanical properties. Changes in surface morphology and reduction in MFI were observed. In conclusion, it may be claimed that the incorporation of piassava fiber in small size particles and nanoclay in the HDPE followed by electron-beam irradiation effectively improved the polymer properties and led to materials with superior properties suitable for several industrial applications.

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  • IPEN-DOC 23861

    PEREIRA, AMANDA C.S. ; CHINELLATO, ANNE C.; SORIA, ALEJANDRA T.; ABREU, ANIBAL V.; DEL MASTRO, NELIDA L. ; MOURA, ESPERIDIANA A.B. . Comparative study of the sugarcane bagasse FIBER/HDPE composite properties using electron-beam and gamma radiation treatments. In: HWANG, JIANN-YANG (Ed.); MONTEIRO, SERGIO N. (Ed.); BAI, CHENGGUANG (Ed.); CARPENTER, JOHN (Ed.); CAI, MINGDONG (Ed.); FIRRAO, DONATO (Ed.); KIM, BUOUNG-GON (Ed.) CHARACTERIZATION OF MINERALS, METALS, AND MATERIALS. 141th; ANNUAL MEETING & EXIBITION, March 11-15, 2012, Orlando, Florida, USA. Proceedings... Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. p. 403-410.

    Abstract: This work presents a comparative study of the thermo-mechanical and morphological properties of sugarcane bagasse fiber/HDPE composite treated with electron-beam and gamma radiation. The composite samples obtained by extrusion and injection molding processes were irradiated at 50 and 90 kGy using either a 1.5 MeV electron beam accelerator or gamma irradiator EMI-9, at room temperature in presence of air. The irradiated and non-irradiated samples were submitted to mechanical and MFI tests, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), sol-gel analysis and X-Rays Diffraction (XRD) and the correlation between their properties was discussed. Improvement of the bagasse fiber/HDPE composite properties, using electron-beam or gamma radiation, was found.

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  • IPEN-DOC 23860

    NOGUEIRA, BEATRIZ R. ; CHINELLATO, ANNE; ORTIZ, ANGEL V. ; PARVEEN, ARIFA; RANGARI, VIJAYA K.; MOURA, ESPERIDIANA A.B. . Thermal and morphological behavior of EVOH/piassava fiber composites. In: HWANG, JIANN-YANG (Ed.); MONTEIRO, SERGIO N. (Ed.); BAI, CHENGGUANG (Ed.); CARPENTER, JOHN (Ed.); CAI, MINGDONG (Ed.); FIRRAO, DONATO (Ed.); KIM, BUOUNG-GON (Ed.) CHARACTERIZATION OF MINERALS, METALS, AND MATERIALS. 141th; ANNUAL MEETING & EXIBITION, March 11-15, 2012, Orlando, Florida, USA. Proceedings... Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. p. 373-380.

    Abstract: Composite consisting of ethylene vinyl alcohol (EVOH) with short piassava fibers were prepared by extrusion process and their thermal and morphological behavior were investigated. The EVOH reinforced with 5 and 10 % of piassava {Attaleafimifera Mart) fiber particle, based on the percentage weight ratio (wt %), untreated and treated with a commercial silane coupling agent, were prepared and the influence of fiber loading and the effect of chemical treatment on their thermal and morphological behavior were evaluated by SEM, DSC and TG analyses. Melt flow index (MFI) of the composites have been determined to evaluate the effects of fiber reinforcement on dynamic viscoelastic melt of the EVOH. In addition, piassava fibers characterization by SEM, FTIR, and organic and inorganic composition have also been carried out. The SEM results of the composites showed surface micrographs without microvoids and a good distribution, dispersion and compatibility between the fillers and the EVOH matrix. Concerning the piassava fibers characterization, the results showed that piassava has higher lignin content than cellulose and hemicelluloses, and around 0.7 % of ash with 52 % of silica (Si02). This high concentration of silica is consistent with the SEM analysis of the fiber that showed a large amount of "silica rings" encrusted on their surface.

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  • IPEN-DOC 23885

    SABUNDJIAN, GAIANE ; BELCHIOR JUNIOR, ANTONIO ; CONTI, THADEU das N. ; ROCHA, MARCELO da S. ; ANDRADE, DELVONEI A. de ; TORRES, WALMIR M. ; MACEDO, LUIZ A. ; UMBEHAUN, PEDRO E. ; MESQUITA, ROBERTO N. de ; LIMA, ANA C. de S. . Heat transfer mode in the core of the Angra 2 nuclear power plant during small break loca obtained with RELAP5 code. In: REUNIÃO DE TRABALHO SOBRE FÍSICA NUCLEAR NO BRASIL, 36., 1-5 de Setembro, 2013, São Sebastião, SP. Resumo... São Paulo: Sociedade Brasileira de Física, 2013. p. 1-1.

    Abstract: This work aims to identify the heat transfer mode with RELAP5/MOD3.2.gama code in the core of Angra 2 facility. The postulate accident is the Loss of Coolant Accident (LOCA) in the primary circuit for Small Break (SB), which is described in Chapter 15 of the Final Safety Analysis Report of Angra 2 (FSAR). The accident consists basically of the total break of the cold leg of Angra 2 facility. The rupture area considered was 380 cm2, which represents 100% of the primary circuit pipe °ow area. The Emergency Core Cooling System (ECCS) e±ciency is also tested in this accident. In this simulation, failure and repair criteria are adopted for the ECCS components in order to verify the system operation e±ciency - preserving the integrity of the reactor core and guaranteeing its cooling - as expected by the project design. SBLOCA accidents are characterized by a fast blowdown in the primary circuit to values that activate the low pressure injection system followed by the water injection from the accumulators. The thermal-hydraulic processes inherent to the accident phenomenon, such as hot leg vaporization and consequently core vaporization cause inappropriate °ow distribution in the reactor core that can lead to reduction in the core liquid level, up to the point that the ECCS is able to re°ood it. This work shows the mode numbers and the wall convection heat transfer used in the RELAP5 code that were accessed during the execution of the program. The results showed that the numerical simulations with RELAP5 were satisfactory and that the ECCS worked e±ciently, guaranteeing the integrity of the reactor core.

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  • IPEN-DOC 23872

    GONÇALVES, VITOR C. ; ZAHN, GUILHERME S. ; GENEZINI, FREDERICO A. . Development of a novel data analysis procedure for half-life measurements. In: REUNIÃO DE TRABALHO SOBRE FÍSICA NUCLEAR NO BRASIL, 36., 1-5 de Setembro, 2013, São Sebastião, SP. Resumo... São Paulo: Sociedade Brasileira de Física, 2013. p. 1-1.

    Abstract: The precise determination of nuclear parameters for nuclides with a long half-life is of extreme relevance, as these nuclides are often used as standard radiation sources for calibration of detector systems, for instance. On the other hand, the determination of a precise value for the half-life of ling-lived nuclei can be di±cult, as it may require that the activity of a single radioactive source be followed for a long period of several months or even years. In a previous paper, the decay of 57Co and 60Co was studied using daily detector veri¯cation data from the Neutron Activation Analysis Lab of IPEN-CNEN/SP. In this analysis, the presence of many outliers in the datasets proved to be a big issue, so a robust data ¯tting procedure was developed. The aim of the present work was to enhance this data ¯tting method. The enhancement was attained using pre-¯tting ¯lters to deal with data points and uncertainties that fall too far from the perceived behavior of the data, thus reducing the possibility that these very gross outliers would interfere with the ¯tting procedure. Moreover, a software implementation of the ¯tting procedure was developed, written in the Python computing language. The aim is that this software, still in an early stage of development, will be able to ¯t experimental data (initially, decay curves) dealing properly with outliers that may exist in the original dataset.

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  • IPEN-DOC 23877

    MELO JUNIOR, A.S.; GATTI, L. ; SEVEGNANI, F.; SATIE, I.; IZIDRO, J.; IANNUZZI, A.. Estudo da emissão veicular de gases de efeito estufa (GEE) em veículos movidos à gasolina. In: INTERNATIONAL WORKSHOP: ADVANCES IN CLEANER PRODUCTION, 4th, May 22-24, 2013, São Paulo, SP. Anais... 2013. p. 1-5.

    Abstract: Saber o comportamento da emissão de gases de efeito estufa (GEE) na atmosfera é importante devido às mudanças climáticas globais e suas consequências para os ecossistemas. Estudos envolvendo a emissão veicular são de grande importância devido ao seu papel na emissão de GEE. O intuito da pesquisa foi determinar a emissão dos principais GEE (CO2, CH4 e N2O) na frota de veículos movidos à gasolina. Por tal, foram estudados dois veículos movidos à gasolina de anos de fabricação diferentes. Os ensaios foram realizados no dinamômetro da CETESB e no IPEN.

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  • IPEN-DOC 23874

    LAINETTI, P.E.O. . Decomposição térmica segura de pesticidas organoclorados por oxidação submersa em sais fundidos. In: INTERNATIONAL WORKSHOP: ADVANCES IN CLEANER PRODUCTION, 4th, May 22-24, 2013, São Paulo, SP. Anais... 2013. p. 1-10.

    Abstract: Este estudo foi motivado pelo interesse atual, em todo o mundo,no desenvolvimento de processos avançados de decomposição de resíduos, categoria na qual o processo aqui descrito se insere. Este interesse decorre da necessidade de processos mais seguros para a decomposição de determinados resíduos, particularmente aqueles considerados perigosos ou que apresentem impacto significativo no meio ambiente. A tecnologia desenvolvida se enquadra em tal princípio, sendo aplicável para a eliminação intrinsecamente segura deresíduos orgânicos perigosos, particularmente os organoclorados, cuja degradação tem apresentado problemas quando da utilização de métodos mais comuns, como a incineração. Pesticidas banidos, obsoletos ou descartados constituem um sério risco ambiental em todo o mundo, principalmente nos países em desenvolvimento. Os HCHs, ou hexaclorociclohexanos, também denominados BHC ou Lindane, são inseticidas organoclorados banidos na maioria dos países nos anos 70 e 80 . É um dos compostos que constituem o grupo dos chamados POPs, ou seja, poluentes orgânicos persistentes, que são regulados internacionalmente pela Convenção da Basiléia. Dentre os principais POPs poderiam ser citados os pesticidas, as dioxinas e os PCBs que representam, segundo a United Nations Industrial DevelopmentOrganization – UNIDO,um dos problemas mais sérios e urgentes a serem enfrentados, devido, por um lado, à sua ampla difusão no meio ambiente e, por outro, em virtude das suas propriedades e características particulares, as quais determinam sua persistência em águas e solos. A United Nations Environmental Protection - UNEP, por exemplo, iniciou uma ação global para o estabelecimento deum tratado internacional para reduzir e/ou eliminar emissões e descargas de 12 POPs específicos, conhecidos também como “dirtydozen” , ou, literalmente, “dúzia suja” (Aldrin, Clordano, Mirex, Dieldrin, DDT, dioxinas, furanos, PCBs, Endrin, heptacloro, BHC e toxafeno), além de estabelecer critérios científicos para a possível inclusão de outros. A oxidação em sais fundidos é um processo que promove uma decomposição mais completa e mais segura de resíduos considerados críticos, como por exemplo, os POPs (poluentes orgânicos persistentes), produtos químicos obsoletos, compostos extremamente energéticos (propelentes e explosivos), etc. Neste processo, o resíduo e o oxidante (ar ou ar enriquecido com oxigênio) são misturados abaixo da superfície de um leito turbulento de sais em fusão. A oxidação ocorre em temperaturas de processo mais baixas que as da incineração convencional, mas que associadas à fase líquida em que ocorrem as reações, são suficientes para promover uma decomposição completa e segura de resíduos perigosos, particularmente os organoclorados. Neste caso, o cloro reage com o sódio, formando cloreto de sódio, que fica retido no banho salino. Neste trabalho, descrevem-se as atividades de construção e desenvolvimento de um reator de sais fundidos, para decomposição de resíduos perigosos, bem como são apresentados alguns resultados da decomposição de pesticidas.

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  • IPEN-DOC 23865

    BERTOLINI, T.C.R.; MAGDALENA, C.P. ; IZIDORO, J.C. ; CUNICO, P.; FUNGARO, D.A. . Adsorção de ácido laranja 8 de solução aquosa sobre zeólitas sintetizadas a partir de cinzas leves de carvão modificadas por surfactante. In: INTERNATIONAL WORKSHOP: ADVANCES IN CLEANER PRODUCTION, 4th, May 22-24, 2013, São Paulo, SP. Anais... 2013. p. 1-10.

    Abstract: A adsorção do corante ácido laranja 8 (AL8) sobre zeólitas de cinzas leves de carvão modificadas por surfactante foi avaliada. As cinzas de carvão utilizadas na síntese da zeólita (ZCC) por tratamento hidrotérmico alcalino foram coletadas nas Usinas Termelétricas Jorge Lacerda (SC) e Figueira (PR). A modificação das zeólitas foi realizada pela mistura de ZCC com o surfactante brometo de hexadeciltrimetilamônio e os materiais obtidos foram zeólita modificada de Jorge Lacerda (ZMJ) e zeólita modificada de Figueira (ZMF). O equilíbrio de adsorção do corante foi atingido após 90 min para a ZMJ e a ZMF. Os dados experimentais ajustaram-se melhor ao modelo cinético de pseudo-segunda-ordem para ambos adsorventes. O equilíbrio de adsorção foi descrito em termos das isotermas de Langmuir e Freundlich e o modelo de Freundlich foi o mais compatível com os dados experimentais para ZMJ e ZMF. As capacidades de adsorção máximas foram 5,29 mg g-1 para o sistema AL8/ZMJ e 1,79 mg g-1 para o sistema AL8/ZMF.

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  • IPEN-DOC 23859

    LEVY, DEBORA; RUIZ, JORGE L.; BROCARDO, GRACIELA; FERREIRA, ADILSON; QUEIROZ, RODRIGO ; MARIA, DURVANEI; BYDLOWSKI, SERGIO; PEREIRA, JULIANA. In vitro and in vivo antitumor effects of Azidothymidine in a human multiple myeloma cell line. In: ANNUAL SCIENTIFIC MEETING/EXPERIMENTAL HEMATOLOGY, 41st, August 23-26, 2012, Amsterdam, Netherlands. Abstract... Chicago, Illinois, USA: International Society for Experimental Hematology, 2012. p. S144-S144.

    Abstract: Azidothymidine (AZT) is an antiretroviral nucleoside analogous inhibitor of reverse transcriptase with known effects on cell proliferation, apoptosis, and angiogenesis Multiple myeloma is a severe disease and one of the steps involved in the malignant transformation of plasma cells is the activation of the nuclear factor kappa B (NF-κB) pathway.

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  • IPEN-DOC 23858

    CARVALHO, A.A.T.; SANTOS, L.F.C.; GUEIROS, L.A.; BARKOKEBAS, A.; FONSECA, D.D.D.; CARDOSO, S.; FREITAS, P.M.; NOGUEIRA, G.E.C. ; LEAO, J.C.. Low-level laser therapy in the treatment of burning mouth syndrome. In: BIENNIAL CONGRESS OF THE EUROPEAN ASSOCIATION OF ORAL MEDICINE, 11th, September 13-15, 2012, Athens, Greece. Abstract... 2012. p. 29-29.

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  • IPEN-DOC 23856

    BONIFACIO, DANIEL A.B.; MORALLES, MAURICIO . Modeling of 3D gamma interaction position in a monolithic scintillator block with a row-column summing readout. In: IEEE NUCLEAR SCIENCE SYMPOSIUM AND MEDICAL IMAGING CONFERENCE RECORD, October 29 - November 3, 2012, Anaheim, CA, USA. Proceedings... Piscataway, NJ: IEEE, 2012. p. 2606-2613.

    Abstract: Current positron emission tomography (PE T ) systems have their detector design based on discrete scintillation crystals. Spatial resolution of these systems are directly related with the size of the crystal segments. On the other hand, cost and complexity increase considerably as the size of the crystal segments decreases. Detector design with continuous scintillator is another approach that improves the energy resolution and sensitivity without degrading the spatial resolution. In this work, we report a method to determine the gamma interaction position with depth of interaction (DOl) capability inside a monolithic crystal coupled to a photodetector array. T he method is based on estimating parameters of a model which describes the signal distribution of the optical photons collected by the photodetector array in a row-column summing readout scheme. Evaluation data were simulated using the GATE framework (Geant4 Application for Emission Tomography) to characterize the detector and to compare the interaction position of the incident gamma with the position registered by the detector.

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  • IPEN-DOC 23857

    CARATIN, R.L. ; MARINUCCI, G. . Analysis of fiber-concrete interface in cement matrix composites. In: EUROPEAN CONFERENCE ON COMPOSITE MATERIALS, 15th, June 24-28, 2012, Venice, Italy. Proceedings... 2012. p. 1-5.

    Abstract: In the present work, the properties of fiber-cement interface were evaluated in order to enable the use of continuous glassfiber-AR (alkali resistant), replacing steel bars often used in concrete structures by civil construction industry. Pull-out tests were applied in a continuous glassfiber-AR (alkali resistant) reinforcement element. The fiber was 250 mm length and it was inserted 50 mm in a cement matrix specimen whose dimensions are 100 x 100 x 100 mm. The bond strength values were compared to concrete specimen that was manufactured corrugated steel bars. Preliminary results identified an increase of 41% in the fiber-cement bond strength value by using glassfiber – AR when compared to steel bar and cement matrix composites.

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  • IPEN-DOC 23855

    BONALUME, B.C.F.; LEBRAO, G.W.; ROSSI, J.L. . Microwave assist functionalization of multi walled carbon nanotubes. In: EUROPEAN CONFERENCE ON COMPOSITE MATERIALS, 15th, June 24-28, 2012, Venice, Italy. Proceedings... 2012. p. 1-8.

    Abstract: There are many technique to increase reactivity of carbon nanotubes, this article study a improvement in oxidation and silanization procedures. Two oxidation methods are frequently used in carbon nanotubes: the conventional acid treatment, and the microwave treatment. The nanotubes oxidation was studied using microwaves and varying power and time of exposure. Compared to conventional acid treatment, the use of microwave provides benefits in speeding the reaction time, lowest amount of acids required and agitation is not needed. The present procedure was successful in the functionalization of carbon nanotubes with 3- aminopropyltriethoxysilane, which is a coupling agents that have a unique feature, bound organic to inorganic molecules.

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  • IPEN-DOC 23897

    LEPINE-SZILY, A.; LEISTENSCHNEIDER, E.; MENDES JUNIOR, D.R.; DESCOUVEMONT, P.; LICHTENTHALER, R.; GUIMARAES, V.; FARIA, P.N. de; BARIONI, A.; PIRES, K.C.C.; MORCELLE, V.; PAMPA CONDORI, R.; MORAES, M.C.; SCARDUELLI, V.; ROSSI, E.; ZAGATTO, V.A.; SANTOS, H.; AGUIAR, V.A.P.; BRITOS, T.; ASSUNÇAO, M.; ZAMORA, J.C.; DUARTE, J.; SHORTO, J.M.B. . Spectroscopy of high lying resonances in 9Be produced with radioactive 8Li beams. In: INTERNATIONAL WORKSHOP ON COMPOUND NUCLEAR REACTIONS AND RELATED TOPICS, 4th, October 07-11, 2013, São Paulo, SP. Proceedings... Les Ulis, France: EDP Sciences, 2014. p. 1-7. (EPJ Web of Conferences, 69, UNSP 00006).

    Abstract: We present the results of the 8Li(p,α)5He and 8Li(p,p)8Li reactions measured at the RIBRAS (Radioactive Ion Beams in Brazil) system. The experiment was realized in inverse kinematics using a thick [CH2]n polyethylene target and an incident 8Li beam, produced by RIBRAS. Using the thick target method, the complete excitation function could be measured between Ecm = 0.2 − 2.1 MeV, which includes the Gamow peak energy region. The excitation function of the 8Li(p,α)5He reaction, populating resonances between 16.888 and 19.0 MeV in 9Be, was obtained[1] and the resonances were fitted using R-matrix calculations. This study shed light on spins, parities, partial widths and isospin values of high lying resonances in 9Be. The measurement of the resonant elastic scattering 8Li(p,p)8Li populating resonances in the same energy region can constrain the resonance parameters. Preliminary results of the elastic scattering are also presented.

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  • IPEN-DOC 23880

    NISTI, M.B. ; SAUEIA, C.H.R. ; MAZZILLI, B.P. ; MENESES, N.F.A.. Assessment of metals by instrumental neutron activation analysis in six fish species consumed in the city of São Paulo. In: INTERNATIONAL CONFERENCE ON HEAVY METALS IN THE ENVIRONMENT, 16th, September 23-27, 2012, Rome, Italy. Proceedings... Les Ulis, France: EDP Sciences, 2013. p. 1-3. (EPJ Web of Conferences, 1, 37007).

    Abstract: The objective of this work is to quantify the concentration of the metals As, Co, Cs, Fe, K, Na, Rb, Sb, Sc, Sm, Th, U and Zn in the muscles of six fish species (anchovy, brycon, catfish, hake, pacu and sardine). Metals characterization was performed by instrumental neutron activation analysis (INAA). The elements determination was made by irradiation of approximately 150 mg of each sample (dry weight), during 16 hours at a neutron flux of 1012 n.cm-2.s-1 in the nuclear research reactor IEA-R1 of Instituto de Pesquisas Energéticas e Nucleares (IPEN). Most of the results for the metals concentration is low, exception for As and Zn in seawater fish and for Rb in river fish. The results obtained in this study can be used to ensure the quality of the fish consumed in the city of São Paulo.

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  • IPEN-DOC 23854

    ANDRADE-II, E.; MENEZES, J.C.M.; DUARTE, S.B.; GARCIA, F.; ROSSI, P.C.R. ; TAVARES, O.A.P.; DEPPMAN, A.. Effects of fissioning nuclei distributions on fragment mass distributions for high energy fission. In: INTERNATIONAL WORKSHOP ON COMPOUND NUCLEAR REACTIONS AND RELATED TOPICS, 3rd, September 19-23, 2011, Prague, Czech Republic. Proceedings... Les Ulis, France: EDP Sciences, 2012. p. 1-9. (EPJ Web of Conferences, 21, 10001).

    Abstract: We study the effects of fissioning nuclei mass- and energy-distributions on the formation of fragments for fission induced by high energy probes. A Monte Carlo code called CRISP was used for obtaining mass distributions and spectra of the fissioning nuclei for reactions induced by 660 MeV protons on 241Am and on 239Np, by 500 MeV protons on 208Pb, and by Bremsstrahlung photons with end-point energies at 50 MeV and 3500 MeV on 238U. The results show that even at high excitation energies, asymmetric fission may still contribute significantly to the fission cross section of actinide nuclei, while it is the dominante mode in the case of lead. However, more precise data for high energy fission on actinide are necessary in order to allow definite conclusions.

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  • IPEN-DOC 23853

    CALIARI, FELIPE R.; CANDIOTO, KATIA C.G.; REIS, DANIELI A.P.; COUTO, ANTONIO A. ; MOURA NETO, CARLOS de; NUNES, CARLOS A.. Study of the mechanical behavior of an Inconel 718 aged superalloy submitted to hot tensile tests. Sae Technical Paper Series, n. 2011-36-0328, 2011. DOI: 10.4271/2011-36-0328

    Abstract: This study aims to determine some mechanical properties of an Inconel 718 aged superalloy obtained through hot tensile tests. These properties as conventional 0.2% yield strength (σy), ultimate strength (σUTS), and specific elongation assessment (εu) are important parameters in the study of the mechanical material behavior. The superalloy was subjected to hot tensile tests at 650, to 700°C and a strain rate of 0.5 mm/min according to ASTM E-8. It is used a scanning electron microscope (SEM) to obtain images of the fracture surface of the specimens. The images of the fracture surface are analyzed in order to relate the temperature of the test with the type of mechanism of fracture.

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  • IPEN-DOC 23869

    CONTI, THADEU das N. ; MENDES, LUIZ F.F. . Desenvolvimento de um programa computacional para cálculo do balanço térmico no Circuito de Circulação Natural CCN do IPEN/POLI durante a operação do circuito. In: SIMPOSIO INTERNACIONAL DE INICIAÇÃO CIENTÍFICA DA USP, 21., 21-25 de outubro, 2014, São Paulo, SP. Resumo... 2014.

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  • IPEN-DOC 23879

    MIYAGUSHI, MASSATOSHI; DEANA, ALESSANDRO M.; RAELE, MARCUS P. ; SILVA, MARILDA F. de S. da. Green technology: I-JOBS manager desimpresora a laser. In: SIMPÓSIO DE INICIAÇÃO CIENTÍFICA E TECNOLÓGICA, 15.; CONGRESSO DA FACULDADE DE TECNOLOGIA DE SÃO PAULO, 15., 07-11 de outubro, 2013, São Paulo, SP. Resumo... São Paulo: FATEC, 2013. p. 98-98.

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  • IPEN-DOC 23878

    MENZEL, FRANCINE ; SABUNDJIAN, GAIANE . Análise de risco considerando acidentes em reatores nucleares e refinarias de petróleo. Revista Brasileira de Energia, v. 19, n. 2, p. 143-153, 2013.

    Abstract: Análises de risco consistem em importantes instrumentos para auxílio na tomada de decisão, principalmente relacionadas às escolhas energéticas e suas consequências ambientais. O objetivo do artigo é analisar o risco associado com a implantação e operação de uma instalação nuclear e comparar com o risco associado de outras fontes de energia, como o petróleo. Foi feita uma análise de risco baseada no número de reatores-ano e no número de acidentes que já ocorreram no mundo em usinas nucleares. O mesmo foi feito baseado no número de refinarias-ano e no número de acidentes que já ocorreram no mundo em refinarias de petróleo. Nossos resultados mostraram que o risco de acidente em uma usina nuclear no mundo é menor quando comparado com o risco associado à produção de petróleo. Acreditamos que a análise proposta pode influenciar os processos de tomada de decisão na área ambiental e contribuir para um futuro energético mais sustentável.

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  • IPEN-DOC 23864

    TYTGAT, JAN; PEIGNEUR, STEVE; BELATO Y ORTS, DIEGO J.; SILVA, ALVARO P. da; OGUIURA, NANCY; BONI-MITAKE, MALVINA ; ZAHARENKO, ANDRE J.. Crotamine toxicity revisited: novel insights based on KV channel inhibition. Biophysical Journal, v. 102, n. 3, supl. 1, p. 658A - 658A, 2012.

    Abstract: Crotamine, a 5KDa peptide possesses a unique biological versatility. Not only its cell-penetrating activity has become of clinical interest but moreover, its potential selective anti-tumor activity is of great pharmacological importance. Before, several studies have attempted to elucidate the exact molecular target responsible for the crotamine-induced skeletal muscle spasm. The aim of this study was to investigate whether crotamine affects voltage-gated potassium (KV) channels in an effort to explain its in vivo effects. Crotamine was studied on ion channel function using the two-electrode voltage clamp technique on 16 cloned ion channels (12 KV channels and 4 NaV channels), expressed in Xenopus laevis oocytes. Crotamine selectively inhibits KV1.1, KV1.2 and KV1.3 channels with an IC50 of ~300 nM and the key amino acids responsible for this molecular interaction are highlighted. Our results demonstrate for the first time that the symptoms which are observed in the typical crotamine syndrome may result from the inhibition of KV channels. The ability of crotamine to inhibit the potassium current through KV channels unravels it as the first snake peptide with the unique multifunctionality such as cell penetrating, antitumoral activity and KV channel inhibiting properties. The potent and selective KV channel inhibiting properties, as demonstrated in this work, can be an advantage for the use of crotamine or its derivatives as antitumor drug. This new property of crotamine might explain some experimental observations and opens new perspectives of pharmacological uses.

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  • IPEN-DOC 23862

    ROCHA, GISELE O. da; VASCONCELLOS, PEROLA C.; AVILA, SIMONE G.; SOUZA, DAVI Z. ; REIS, EDUARDO A.O.; OLIVEIRA, PEDRO V.; SANCHEZ-CCOYLLO, ODON. Seasonal distribution of airborne trace elements and water-soluble ions in São Paulo Megacity, Brazil. Journal of the Brazilian Chemical Society, v. 23, n. 10, p. 1915-1924, 2012. DOI: 10.1590/S0103-50532012005000062

    Observação: Correção no final do artigo citado em Journal of the Brazilian Chemical Society, v. 23, n.11, p. 2129, 2012.

    Abstract: This study deals with the seasonal distribution of Al, Ca, Cu, Fe, K, Mg, Na, Pb and Zn and water soluble ions (Cl-, PO4 3-, NO3 -, SO4 2-, HCOO-, CH3COO-, oxalate, succinate, Na+, NH4 +, K+, Mg2+ and Ca2+) found in PM10 samples (particulate matter less than 10 mm in diameter) São Paulo City, Brazil, (April 2003-May 2004). Higher atmospheric levels were found for SO4 2-, NO3 -, Cl- and PO4 3- while the main organic anions were oxalate and formate. Atmospheric levels for elements were: Fe > Al > Ca > K > Na > Mg > Zn > Cu > Pb. Some sources were predominant for some species: (i) fuel burning and/or biomass burning (NO3 -, HCOO-, C2O4 2-, K+, Mg2+, Ca2+, Fe, Pb, Zn, Al, Ca, K and Mg), (ii) gas-to-particle conversion (SO4 2- and NH4 +) and (iii) sea salt spray (Cl-, Na+ and Na).

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  • IPEN-DOC 23851

    ZAHN, GUILHERME S. ; GENEZINI, FREDERICO A. ; TICIANELLI, REGINA B. ; SAIKI, MITIKO . Long-term performance assessment of hpge detectors used in the neutron activation analysis (LAN) of IPEN-CNEN/SP (Brazil). In: ENCONTRO DE FISICA, 03-07 de setembro, 2016, Natal, RN. Abstract... São Paulo: Sociedade Brasileira de Física, 2016.

    Abstract: In nuclear spectroscopy measurements, the performance of the radiation detectors employed is a key issue in the final results; moreover, in comparative measurements, the results depend on the stability of the detector between measurements, so that the detector’s efficiency can be effectively ruled out of the equation. Due to the relevance of the detectors' performances, LAN-IPEN have been performing daily verification measurements in its operational detectors since 1999; this verification consists in the measurement of composite 57+60Co sources, in which the position, resolution and CPS (counts per second) for the 122 keV peak of 57Co and the 1332 keV peak of 60Co are registered, along with the date and time of the measurement. In this work, verification data for 11 HPGe detectors from two different makers and three different intrinsic configurations were analyzed in respect to the efficiency stability (determined by the arbitrary efficiency calculated by correcting the CPS for the isotope's decay) and resolution for both peaks, as well as the ratio between the efficiencies for 122 keV and 1332 keV. The results allow a discussion about the stability of these parameters over time (in some cases, more than 15 years), their sensitivity to imminent detector failures and their performance after a failure has been corrected; moreover, the results show a clear correlation between the maker or configuration and the long-term performance of the detector.

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  • IPEN-DOC 23850

    TANIGUSHI, ESTHER; FARIA, FERNANDA; PIMENTA, DANIEL; FANHANI, MARCELA; ZAMBONI, CIBELE B. ; MENDONÇA, RONALDO Z.; SIMONS, SIMONE M.. Characterization of inorganic elements, proteins and hemostatic activity present in coxal fluid of Ornithodoros brasiliensis (Acari: Arrgasidae). In: REUNIÃO CIENTIFICA ANUAL DO INSTITUTO BUTANTAN, 18., 31 de novembro - 02 de dezembro, 2016, São Paulo, SP. Abstract... 2016.

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  • IPEN-DOC 23841

    LEAO, A.R.; ZAMBONI, C.B. ; SILVA, D.G.N.; MEDEIROS, I.M.M.A. ; WEN, F.H.; ALINE, A.V.; MENDONÇA, R.Z.; SIMONS, S.M.. Quantification of Cl and Na in the antigen used for the production of serum antilonomic. In: REUNIÃO CIENTIFICA ANUAL DO INSTITUTO BUTANTAN, 18., 31 de novembro - 02 de dezembro, 2016, São Paulo, SP. Abstract... 2016.

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  • IPEN-DOC 23848

    REIS, S.L. ; MUCCILLO, E.N.S. . Electrical conductivity of La0.9Sr0.1Ga0.8Mg0.2O3-delta with small additiions of strontium gallate. In: ELECTROCERAMICS, 15th, June 27-29, 2016, Limoges, France. Abstract... 2016.

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  • IPEN-DOC 23847

    PIERETTI, E.F.; COZZA, R.C.; NEVES, M.D.M. . Biotribological behaviour evaluation of the ISO 5832-1 stainless steel for biomedical applications treated by optical fiber laser. In: INTERNATIONAL CONFERENCE ON BIOTRIBOLOGY, 3rd, September 11-14, 2016, London, UK. Abstract... 2016.

    Abstract: The present study evaluated the influence of an optical fiber laser surface treatment process on the tribological behaviour of the ISO 5832-1 austenitic stainless steel (SS) on the coefficient of friction and coefficient of wear in ball-cratering wear tests. For comparison reasons, surfaces non treated by laser were also evaluated. A phosphate buffer solution (PBS) was used as electrolyte. Samples of this biomaterial were treated by alternating the laser frequency in order to find out a condition that improves its tribological behaviour. Micro-abrasive wear tests were carried out with a ball of AISI 316L SS (diameter of 25.4 mm – 1”), used as counter-body, and abrasive slurries prepared with abrasive particles of Al2O3 and distilled water. The sample’s surfaces finishing were analyzed by optical and electronic (SEM-EDX) microscopy. The surface characterization showed microstructure modification due to the high temperatures involved in the laser process and the micro-abrasive wear results indicated that the tribological behaviour is influenced by the frequencies values used for the laser surface treatment.

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  • IPEN-DOC 23846

    NAVARRO, RAPHAELLA; MOURA, TIAGO F.A.; YOSHITO, WALTER K. ; ICHIKAWA, RODRIGO ; MARTINEZ, LUIZ G. ; MATOS, RONALDO V.R.; MIRANDA, JOSE R. de A.; SAEKI, MARGARIDA J.. The influence of synthesis parameters on the structure and acb response of Mn0.75 Zn0.25FeyO4 ferrite. In: INTERNATIONAL MATERIALS RESEARCH CONGRESS, 25th, August 14-19, 2016, Cancún, Mexico. Abstract... 2016.

    Abstract: The interest to use magnetic nanoparticles in biomedicine has increased due to their inducible magnetic properties[1-4]. In the treatment of certain diseases as cancer, it has been used to constitute drug carrier systems[2,3] as well as hyperthermia agents[3,4]. These materials can also be used as a tracer in the diagnosis by Magnetic Resonance Imaging (MRI)[3,4] and Alternating Current Biosuceptometry (ACB)5, which are techniques that do not use ionizing radiation. Neverthless, the sensitivity of these techniques depend on the magnetic susceptibility of tracers. In this work, the ferrite nanoparticles with the composition of Mn0.75Zn0.25FeyO4 (ferrite), where 1.5 ? y ? 2.8, were synthesized by the co-precipitation method and the influence of iron content, reaction time and concentration of precipitating agent on the structure and ACB response was analyzed. It was noted that the synthesis using alkaline metal hydroxide between 0.1 and 0.2 mol/L provides single-phase materials with spinel structure [ICSD 28515 (PDF - 742 402), space group Fd3m]. The higher concentration (0.2 mol/L) leads to materials with higher crystallinity and adequate performance for the ACB technique. The longer the reaction time the greater is the magnetic susceptibility for compositions wherein the iron is in excess (y>2.0). Also, the susceptibility is higher for ferrites with excess of iron.

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  • IPEN-DOC 23845

    MOREIRA, TATIANA M. ; SEO, EMILIA S.M. . Reúso da água de chuva: uma alternativa sustentável para os períodos de escassez hídrica / Reuse of rain water: a sustainable alternative for the periods water shortage. In: SIMPÓSIO INTERDISCIPLINAR DE CIÊNCIA AMBIENTAL, 2., 14-16 de setembro, 2016, São Paulo, SP. Anais... São Paulo: IEE/USP, 2016. p. 547-558.

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  • IPEN-DOC 23844

    MOREIRA, E.G.; VASCONCELLOS, M.B.A. ; ROSA, M.M.L.; TADDEI, M.H.T.. Naturally occurring radionuclides in a Brazilian mussel reference material. In: NATURALLY OCCURRING RADIOACTIVE MATERIAL, 8th, October 18-21, 2016, Rio de Janeiro, RJ. Proceedings... 2016.

    Abstract: Certified reference materials are important tools for the quality assurance of analytical results. However there are several constraints for their widespread use in developing countries such as technological development or difficult access to imported goods. Another issue is that analyte level differences between imported certified reference materials and local laboratory samples may be a concern in the measurement process. This contribution describes the naturally occurring activity concentrations of 234U, 235U, 238U and 232Th, determined by Alpha Spectrometry after a radiochemical separation procedure and 40K and 210Pb determined by Gamma Ray Spectrometry in a Perna perna mussel reference material produced in Brazil. Obtained activity concentrations were included as information values in the certification process of the reference material.

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É possível efetuar a busca de um autor ou um termo em todo o RD, por meio do Buscar no Repositório , isto é, o termo solicitado será localizado em qualquer campo do RD. No entanto esse tipo de pesquisa não é recomendada a não ser que se deseje um resultado amplo e generalizado.

A pesquisa apresentará melhor resultado selecionando um dos filtros disponíveis em Navegar

Os filtros disponíveis em Navegar tais como: Coleções, Ano de publicação, Títulos, Assuntos, Autores, Revista, Tipo de publicação são autoexplicativos. O filtro, Autores IPEN apresenta uma relação com os autores vinculados ao IPEN; o ID Autor IPEN diz respeito ao número único de identificação de cada autor constante no RD e sob o qual estão agrupados todos os seus trabalhos independente das variáveis do seu nome; Tipo de acesso diz respeito à acessibilidade do documento, isto é , sujeito as leis de direitos autorais, ID RT apresenta a relação dos relatórios técnicos, restritos para consulta das comunidades indicadas.

A opção Busca avançada utiliza os conectores da lógica boleana, é o melhor recurso para combinar chaves de busca e obter documentos relevantes à sua pesquisa, utilize os filtros apresentados na caixa de seleção para refinar o resultado de busca. Pode-se adicionar vários filtros a uma mesma busca.

Exemplo:

Buscar os artigos apresentados em um evento internacional de 2015, sobre loss of coolant, do autor Maprelian.

Autor: Maprelian

Título: loss of coolant

Tipo de publicação: Texto completo de evento

Ano de publicação: 2015

Para indexação dos documentos é utilizado o Thesaurus do INIS, especializado na área nuclear e utilizado em todos os países membros da International Atomic Energy Agency – IAEA , por esse motivo, utilize os termos de busca de assunto em inglês; isto não exclui a busca livre por palavras, apenas o resultado pode não ser tão relevante ou pertinente.

95% do RD apresenta o texto completo do documento com livre acesso, para aqueles que apresentam o significa que e o documento está sujeito as leis de direitos autorais, solicita-se nesses casos contatar a Biblioteca do IPEN, bibl@ipen.br .

Ao efetuar a busca por um autor o RD apresentará uma relação de todos os trabalhos depositados no RD. No lado direito da tela são apresentados os coautores com o número de trabalhos produzidos em conjunto bem como os assuntos abordados e os respectivos anos de publicação agrupados.

O RD disponibiliza um quadro estatístico de produtividade, onde é possível visualizar o número dos trabalhos agrupados por tipo de coleção, a medida que estão sendo depositados no RD.

Na página inicial nas referências são sinalizados todos os autores IPEN, ao clicar nesse símbolo será aberta uma nova página correspondente à aquele autor – trata-se da página do pesquisador.

Na página do pesquisador, é possível verificar, as variações do nome, a relação de todos os trabalhos com texto completo bem como um quadro resumo numérico; há links para o Currículo Lattes e o Google Acadêmico ( quando esse for informado).

ATENÇÃO!

ESTE TEXTO "AJUDA" ESTÁ SUJEITO A ATUALIZAÇÕES CONSTANTES, A MEDIDA QUE NOVAS FUNCIONALIDADES E RECURSOS DE BUSCA FOREM SENDO DESENVOLVIDOS PELAS EQUIPES DA BIBLIOTECA E DA INFORMÁTICA.

O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.

A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

Repositórios Digitais Institucionais: a experiência

do Instituto de Pesquisas Energéticas e Nucleares

(IPEN)

Mery P. Zamudio Igami

Biblioteca Terezine Arantes Ferraz

1 Repositórios Digitais Institucionais – RDI

A tecnologia da informação modificou significativamente todas as atividades rotineiras da sociedade. O surgimento das redes de comunicação transformou o ambiente onde elas atuam mudando o conceito de espaço e tempo no âmbito da comunicação e informação pela instantaneidade da transmissão de dados.

Na área de comunicação científica não foi diferente. Toda a cadeia de produção, disseminação, publicação e armazenamento de documentos científicos experimentou uma grande transformação, impactando de forma positiva a produção e consumo da informação científica na área acadêmica. O surgimento da internet e da WWW, na década de 80, aceleraram as mudanças na forma de comunicar e disseminar os trabalhos acadêmicos que passaram do suporte exclusivamente em papel para o suporte digital.

Na mesma época houve uma significativa crise mundial devido aos altos preços praticados pelas editoras no processo de assinatura das publicações periódicas, tornando inviável a sustentação das coleções pelas bibliotecas e afetando diretamente a consulta pelos usuários. Como reação da própria comunidade científica surgiu então o movimento de livre acesso à informação científica, mais conhecido por Open Access Iniciative (KURAMOTO, 2012).

Esse movimento defende a disponibilização na internet da literatura acadêmica e científica permitindo que seja lida, descarregada, distribuída, impressa e pesquisada livremente.

É importante o registro histórico desse movimento não somente porque está diretamente relacionado à criação dos RDIs, mas também porque ele possibilitou várias mudanças estruturais no fluxo da comunicação científica. Os editores perderam a exclusividade de distribuição (comercialização) da pesquisa científica, uma vez que na era digital é possível que isso seja realizado de forma descentralizada e por iniciativa de cada autor.

1.1 O movimento do Open Access Iniciative - OAI

A partir de 1999, os movimentos em favor do acesso livre à literatura científica, principalmente à literatura produzida sob financiamento público, consolidaram-se.

A comunidade acadêmica envolvida nesses movimentos corroborou o seu apoio por meio de declarações formais, dentro das quais se destaca a Declaração de Budapest – BOAI –1 em 2002, onde foram emitidos os conceitos básicos do OAI, e a Declaração de Berlim2 em 2003, onde se destacou a natureza institucional dos repositórios digitais de livre acesso como um espaço organizado baseado em regras de utilização que conferem credibilidade aos materiais publicados e em políticas de arquivamento a longo prazo.

O movimento Open Archives Iniciative desenvolveu padrões de interoperabilidade buscando facilitar a comunicação eficiente de conteúdo. Foram também estabelecidas duas modalidades para viabilizar a disponibilidade dos conteúdos: a via verde que diz respeito ao autodepósito nos RDs e a via dourada que inclui as revistas que publicam os artigos com livre acesso.

O primeiro repositório digital (ArXiv.org )3 foi instalado no laboratório de física, em Los Álamos, EU, coordenado pelo físico Paul Ginsparg (1996), motivado pela insatisfação com os rumos na política de aquisição das publicações científicas e a crescente inviabilidade das bibliotecas americanas de manter as coleções atualizadas. Daquela época para a atual aconteceram inúmeras mudanças positivas nos modelos de funcionamento dos repositórios promovidas pela evolução da TI.

________________________________________________

1 Budapest Open Access Iniciative - http://www.budapestopenaccessinitiative.org

2 Open Access - http://openaccess.mpg.de/Berlin-Declaration

3 General Information about Arxiv - http://arxiv.org

Não existe um modelo único para a criação dos RDIs; cada instituição define a política de depósito adequada ao contexto da sua comunidade. O importante é que essa comunidade valide e utilize a plataforma tornando-a uma vitrine da sua produção científica.

No país, o movimento de Acesso Aberto e construção de Repositórios Digitais Institucionais tiveram um grande incentivo e apoio técnico do Instituto Brasileiro de Informação em Ciência e Tecnologia – (IBICT) (INSTITUTO BRASILEIRO DE CIÊNCIA E TECNOLOGIA, 2009). De acordo com o Diretório internacional de RDIs – o DOAR4, até o momento, há no país 153 repositórios digitais em funcionamento, dos quais a maioria é mantida pelas instituições onde a comunidade produz os trabalhos científicos. Desses RDIs, 74% utilizam o software Dspace5.

1.2 O Projeto de Lei sobre os Repositórios Digitais

Em 2007, foi apresentado o Projeto de Lei no senado PL1120/2007 (BRASIL, 2007), que torna obrigatório nas universidades e institutos de pesquisa o desenvolvimento e implantação de RDIs nas instituições de origem, bem como torna obrigatório o autodepósito da produção científica por parte dos seus pesquisadores. Além disso, o Projeto de Lei determina a formação de uma comissão de alto nível para discutir, propor e estabelecer uma política de livre acesso à informação científica. Além de simplesmente criar mecanismos de disseminação da informação científica, o Projeto propõe a criação de uma comissão para discutir políticas e estabelecer diretrizes a serem adotadas em nível nacional pelas instituições que produzem informação científica.

1.3 O que são os RDIs?

Os RDIs constituem atualmente um avanço na gestão do conhecimento intelectual. Podem receber diversas denominações tais como equipamentos institucionais, observatórios digitais, instrumentos ou ferramentas de gestão de conhecimento e outras.

________________________________________________

4 The Directory of Open Access Repositories – OpenDOAR - http://www.opendoar.org

5 Software livre para construção de Repositorios Institucionais Digitais (Dspace) - http://www.ibict.br/pesquisa-desenvolvimento-tecnologico-e-inovacao/Sistema-para-Construcao-de-Repositorios-Institucionais-Digitais

Um dos aspectos mais importantes dos RDIs é que eles estão inseridos na política institucional de gestão do conhecimento e são essencialmente instrumentos gerenciais de onde se obtém indicadores para auxiliar na gestão da política científica das instituições de pesquisa.

Sob a ótica documentária um RDI constitui um serviço de informação científica em ambiente digital, interoperável e de acesso aberto, dedicado ao gerenciamento da produção científica de uma instituição. Contempla a reunião, armazenamento, organização, preservação, recuperação e, sobretudo, a ampla disseminação e visibilidade da informação científica produzida na instituição.

1.3.1 O que caracteriza um repositório digital?

a) a organização, armazenamento, acesso e distribuição de documentos digitais;

b) a utilização de padrões abertos acessibilidade;

c) as funcionalidades que o software selecionado permite;

d) a utilização de metadados com padrão internacional – interoperabilidade;

e) o arquivo digital anexado;

f) o acesso de buscadores para elaboração de rankings internacionais - visibilidade;

g) o autodepósito dos trabalhos;

h) a monitoração, controle e elaboração de indicadores.

2 O RDI do IPEN

O prestígio de uma instituição de pesquisa está fortemente vinculado aos resultados dos elementos intangíveis, por exemplo, qualidade no ensino da pós-graduação, e tangíveis como os resultados da pesquisa, entre eles a Produção científica. Tradicionalmente, o IPEN, por meio da sua unidade de informação, mantém a sua produção científica organizada e atualizada desde sua criação em 1956. No entanto, devido ao avanço da tecnologia da informação, tornou-se necessário atualizar a plataforma gerenciadora dessa informação já armazenada, com a consequente modernização no processo de gestão do capital intelectual.

No decorrer de 2013, seguindo outras instituições no país, como por exemplo, a Universidade de São Paulo, a Universidade Estadual Paulista e a Universidade Estadual de Campinas, a Direção do IPEN decidiu criar o seu repositório, uma vez que a matéria prima para concretizar esta ação já se encontrava disponível e organizada de tal forma que se garantiria a sustentabilidade do RDI.

A política de gestão da produção científica é uma prática rotineira no IPEN. Diferentemente da maioria dos repositórios em funcionamento, o RDI do IPEN já nasce com um volume significativo de matéria-prima digital organizada, com políticas definidas de auto arquivamento e de preservação da produção científica.

Porém, o fator determinante de sucesso foi o modelo de indexação e organização dos documentos adotado pela biblioteca desde o início em 1976, quando ainda se constituía em uma base de dados referencial. Este fato facilitou o processo de migração e compatibilização dos metadados para o novo padrão internacional adotado, o Dublin Core, garantindo interoperabilidade com outros repositórios digitais, uma das principais características dos RDIs.

Em novembro de 2013, foram iniciados os trabalhos de construção do RDI do IPEN. Inicialmente foram programadas visitas do grupo de informática e da biblioteca a instituições com experiências bem-sucedidas, como, por exemplo, o Instituto de Pesquisas Espaciais – (INPE) em São José dos Campos e a Universidade de São Paulo- USP, com o objetivo de conhecer melhor o projeto de RDIs dessas instituições e selecionar o modelo mais adequado à realidade do IPEN.

As visitas técnicas embasaram a decisão de selecionar o software Dspace para confecção do repositório do IPEN. O Dspace é um software livre especializado no gerenciamento de repositórios resultante de um projeto colaborativo da MIT Libraries e da Hewlett-Packard Company, amplamente utilizado no mundo. No Brasil, graças ao incentivo do IBICT em Brasília, o DSpace é utilizado por 75% das instituições que possuem repositórios.

2.1 O processo de migração

Em dezembro de 2013, foi iniciado o processo de criação do novo repositório do IPEN, após um estudo preliminar do software e contratação de uma empresa para a capacitação das equipes da biblioteca e da informática.

Nos meses seguintes foram efetuados os testes de implantação do Dspace, configuração, desenho da nova arquitetura do banco de dados, compatibilização e criação de novos metadados, desenvolvimento de funcionalidades.

Na época, a produção científica contabilizada era de 19.200 itens. A migração gradual desses itens iniciou-se em julho de 2014.

Internamente, além de todas as funções inerentes e habituais a um repositório, o diferencial do RDI do IPEN diz respeito à integração com os dados registrados no sistema de informação gerencial e planejamento do instituto, o SIGEPI. Trata-se da uma funcionalidade especial, desenvolvida em ambiente externo ao Dspace, porém acrescentada no momento do registro do documento no Dspace via metadado específico, de tal forma que cada trabalho registrado no RD se correlaciona, automaticamente com a atividade do Plano Diretor à qual o autor do IPEN está vinculado.

Para essa finalidade foi efetuado um exaustivo trabalho de identificação dos nomes completos dos autores do IPEN (lista de autoridades), com as suas respectivas variáveis na forma de citação e agrupamento dos trabalhos. Foi atribuído um número único de identificação para cada autor, o ID autor IPEN, formato semelhante ao Researcher ID utilizado pela base internacional Web of Science. Essa facilidade constituiu um filtro que ao ser acionado reúne e apresenta rapidamente todos os trabalhos de um determinado autor.

Ao RDI está vinculada também uma tabela estatística, atualizada on-line a cada registro introduzido. Essa funcionalidade destina-se à obtenção de dados para a elaboração de índices e quadros estatísticos a serem confeccionados fora do ambiente do Dspace para subsidiar os pesquisadores e os gestores do IPEN, com informações para a monitoração, administração e elaboração de indicadores científicos fornecidos para o Plano Diretor do IPEN, o relatório anual da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior( CAPES) e outros, bem como para cumprir as exigências dos órgãos financiadores de pesquisa.

O modelo adotado para a coleta dos trabalhos também difere de outros. Nesta primeira etapa foi considerado mais conveniente o serviço de informação (biblioteca) responsabilizar-se pela coleta, depósito e complementação dos dados, utilizando as fontes de informação nacionais e internacionais. Assim, a unidade de informação realiza periodicamente a verificação dos trabalhos publicados pelos pesquisadores nas bases Web of Science, Scopus, Scielo, INIS e Currículo Lattes. Após a eliminação das duplicatas e localização dos documentos na íntegra efetua-se o depósito no RDI.

Trata-se de um modelo de depósito que esta sendo testado, há vantagens e desvantagens. A principal vantagem diz respeito à economia de tempo do pesquisador uma vez que se elimina a obrigatoriedade do autodeposito, tornando, porém, imprescindível por parte do pesquisador à atualização do seu Currículo Lattes. A maior desvantagem foi absorvida pela Biblioteca, qual seja a coleta periódica dos documentos publicados bem como a localização e obtenção da evidência dos mesmos.

Importante registrar que a construção do RD foi uma iniciativa muito nova na instituição, que demandou um trabalho detalhado, cuidadoso e gradual das equipes de desenvolvimento de sistemas e da biblioteca, dado a pouca familiaridade com a nova plataforma. Alia-se a esse fato a opção de utilizar um software livre, onde certas funcionalidades ainda não foram desenvolvidas.

Em setembro de 2014, após oito meses de trabalho, as equipes conseguiram migrar 23%, das coleções, foi uma tarefa que demandou ajustes, adequações, testes e decisões internas, dado o grande nível de detalhamento dos dados, motivo de comemoração das equipes, após cada etapa finalizada e bem sucedida. Contamos também com a assessoria de uma empresa especializada no assunto, a qual guiou a equipe da informática em cada funcionalidade desenvolvida. Nesse ritmo de trabalho, foi possível completar o processo de migração em fevereiro de 2015. Finalmente, em junho desse mesmo ano, o RDI foi disponibilizado na Internet.

2.2 Desempenho do RDI em 2015

Atualmente o novo RDI:

- dispõe de uma identidade própria e permanente, (ex: http://repositorio.ipen.br );

- utiliza as estatísticas de uso do Google Analytics;

- é reconhecido pelo Google Scholar e outras máquinas de busca da Internet;

- participa dos rankings internacionais de repositórios institucionais (Ex: Webometrics);

- tem registro nos Diretórios Internacionais de RIs (Ex. DOAR);

- interopera com sistemas congêneres.

Conforme foi mencionado, a criação do RDI e a migração dos dados constitui a primeira etapa dentro de um projeto maior, qual seja institucionalizar e operacionalizar a gestão da produção científica do IPEN. Após o processo se consolidar em entrar em rotina de funcionamento, as próximas etapas a serem desenvolvidas pelas equipes, dizem respeito à:

- automação da coleta dos documentos;

- otimização das ferramentas de busca;

- obtenção de relatórios estatísticos customizados;

- otimização do lay-out do RDI;

- acréscimo de novas coleções;

- desenvolvimento de novas funcionalidades.

A pesquisa no RDI é muito simples e segue os padrões da maioria das bases de dados. É possível pesquisar por autor, título, assunto, ID de autor IPEN, ano de publicação, combinar palavras- chave de busca e assim por diante. Apresenta também uma tabela estatística funcionando em tempo real, distribuída por nível monográfico; isso permite que a direção e o corpo técnico científico acompanhe a evolução quantitativa dos documentos depositados no RDI.

Uma das últimas funcionalidades acrescentadas ao RD, diz respeito à sinalização dos autores do IPEN. Ao clicar no símbolo do autor é possível acessar a página do pesquisador, onde se apresentam os seguintes dados: as variáveis do nome, uma tabela com todos os trabalhos constantes no RDI , bem como um quadro resumo numérico. Dessa forma o autor pode visualizar, acessar e monitorar à sua produção científica com o texto completo de forma rápida e simples . O quadro resumo se configura ainda como uma linha do tempo para o autor.

Por se constituir em um equipamento totalmente apoiado na tecnologia da informação, o RDI é um recurso institucional dinâmico sujeito a constantes atualizações e melhorias, o qual deve garantir a confiabilidade dos dados providos bem como assegurar a sua sustentabilidade.

3 Referências bibliográficas

BRASIL. Projeto de Lei do Senado 1120/2007 Dispõe sobre o processo de disseminação da produção técnico-científica pelas instituições de ensino superior no Brasil e dá outras providências. Diário oficial da Republica Federativa , Brasília, DF, Disponível em: http://www.camara.gov.br/sileg/integras/461698.pdf Acesso em: set. 2014.

INSTITUTO BRASILEIRO DE INFORMAÇÃO EM CIÊNCIA E TECNOLOGIA - IBICT Repositórios digitais. Site institucional. Brasília: 2009. Disponível em: http://www.ibict.br/informacao-para-ciencia-tecnologia-e-inovacao%20/repositorios-digitais Acesso em set. 2014.

KURAMOTO, H. Acesso livre: como tudo começou. Brasília, 2012. Blog. Disponível em: http://kuramoto.blog.br/2012/02/27/acesso-livre-como-tudo-comecou Acesso em: set. 2014.

SP, janeiro de 2016