PATRICK JACK SPENCER
Resumo
Possui graduação em Ciências Biológicas pela Universidade Presbiteriana Mackenzie (1991), mestrado em Tecnologia Nuclear pela Universidade de São Paulo (1995) e doutorado em Tecnologia Nuclear pela Universidade de São Paulo (2000) tendo sido bolsista sandwich no US Army Medical Research Institute for Infeccious Diseases (98-99). É responsável pelo Biotério de criação e manutenção de animais de laboratório do IPEN. Tem experiência na área de Bioquímica, com ênfase em Proteínas, atuando principalmente nos seguintes temas: veneno, proteínas, bothrops, irradiação e miotoxina.(Texto extraído do Currículo Lattes em 22 dez. 2021)
Projetos de Pesquisa
Unidades Organizacionais
Cargo
42 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 42
Artigo IPEN-doc 27113 Molecular model of cytotoxin-1 from Naja mossambica mossambica venom in complex with chymotrypsin2015 - MUNAWAR, AISHA; AKREM, AHMED; HUSSAIN, ASHIQ; SPENCER, PATRICK; BETZEL, CHRISTIANSnake venom is a myriad of biologically active proteins and peptides. Three finger toxins are highly conserved in their molecular structure, but interestingly possess diverse biological functions. During the course of evolution the introduction of subtle mutations in loop regions and slight variations in the three dimensional structure, has resulted in their functional versatility. Cytotoxin-1 (UniProt ID: P01467), isolated from Naja mossambica mossambica, showed the potential to inhibit chymotrypsin and the chymotryptic activity of the 20S proteasome. In the present work we describe a molecular model of cytotoxin- 1 in complex with chymotrypsin, prepared by the online server ClusPro. Analysis of the molecular model shows that Cytotoxin-1 (P01467) binds to chymotrypsin through its loop I located near the N-terminus. The concave side of loop I of the toxin fits well in the substrate binding pocket of the protease. We propose Phe10 as the dedicated P1 site of the ligand. Being a potent inhibitor of the 20S proteasome, cytotoxin-1 (P01467) can serve as a potential antitumor agent. Already snake venom cytotoxins have been investigated for their ability as an anticancer agent. The molecular model of cytotoxin-1 in complex with chymotrypsin provides important information towards understanding the complex formation.Artigo IPEN-doc 26265 Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to gamma radiation by Cobalt 602019 - SARTORI, GISELLE P.; COSTA, ANDREA; MACARINI, FERNANDA L.S.; MARIANO, DOUGLAS O.C.; PIMENTA, DANIEL C.; SPENCER, PATRICK J.; GALISTEO JUNIOR, ANDRES J.Tetanus is a neurological disease which blocks the inhibitory neurotransmitters liberation. Also treatment does not cure the disease, and its main form of prevention is through vaccination. The vaccine is produced by the inactivation of tetanic toxin (TeNT) with formaldehyde, which may cause side effects. An alternative way is the use of ionizing radiation for inactivation of the toxin and also to improve the potential immunogenic response and to reduce the pos-vaccination side effects. Therefore, the aim of this study was to characterize the TeNT structure after different doses of ionizing radiation of Cobalt 60, and also to assess the enzymatic activity after the radiation. SDS PAGE and MALDI-TOF analysis revealed gradual modification on the TeNT structure according to doses increase. Also, fragmentation and possible aggregations of the protein fragments were observed in higher doses. In the analysis of peptide preservation by enzymatic digestion and mass spectrometry, there was a slight modification in the recognition up to the dose of 4 kGy at subsequent doses, recognition was minimal. The analysis of the enzymatic activity by fluorescence showed a 35 % attenuation in the activity even at higher doses. In the antigenic evaluation performed by ELISA and Western Blot, anti-TeNT antibodies were detected against the irradiated toxins at the different doses, with a gradual decrease as the dose increased, but remaining at satisfactory levels, indicating the possibility of possible use as an immunogen, however, studies of enzymatic activity on higher should be further analyzed.Resumo IPEN-doc 22122 Estudo dos efeitos da radiação gama de sup(Co na bothropstoxina 1 do veneno de Bothrops jararacussu2007 - SILVA, DIEGO de A.; SILVA, MURILO C. da; SPENCER, PATRICK J.Resumo IPEN-doc 22048 Clonagem, sequenciamento e expressão de um bloqueador de cálcio da aranha Phoneutria nigriventer2006 - CALVO, FERNANDA B.; SPENCER, PATRICK J.Resumo IPEN-doc 21477 Isolamento das toxinas do veneno de Crotalus durissus terrificus2015 - FERNANDES, MARINA G.; SPENCER, PATRICK J.Artigo IPEN-doc 21327 Venomics of the Australian eastern brown snake (Pseudonaja textilis): Detection of new venom proteins and splicing variants2015 - VIALA, VINCENT L.; HILDEBRAND, DIANA; TRUSCH, MARIA; FUCASE, TAMARA M.; SCIANI, JULIANA M.; PIMENTA, DANIEL C.; ARNI, RAGHUVIR K.; SCHLUTER, HARTMUT; BETZEL, CHRISTIAN; MIRTSCHIM, PETER; DUNSTAN, NATHAN; SPENCER, PATRICK J.Artigo IPEN-doc 21288 Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: Evidence of a broad arsenal of toxins2015 - VIALA, VINCENT L.; HILDEBRAND, DIANA; FUCASE, TAMARA M.; SCIANI, JULIANA M.; PREZOTTO NETO, JOSE P.; RIEDNER, MARIA; SANCHES, LEONARDO; NISHIMURA, PAULA J.; OGUIURA, NANCY; PIMENTA, DANIEL C.; SCHLUTER, HARTMUT; BETZEL, CHRISTIAN; ARNI, RAGHUVIR K.; SPENCER, PATRICK J.Artigo IPEN-doc 20886 New insights into the structural characteristics of irradiated crotamine2015 - OLIVEIRA, KARINA C.; SPENCER, PATRICK J.; FERREIRA JUNIOR, RUI S.; NASCIMENTO, NANCIArtigo IPEN-doc 20303 Pseudechis guttatus venom proteome: Insights into evolution and toxin clustering2014 - VIALA, VINCENT L.; HILDEBRAND, DIANA; TRUSCH, MARIA; ARNI, RAGHUVIR K.; PIMENTA, DANIEL C.; SCHLUTER, HARTMUT; BETZEL, CHRISTIAN; SPENCER, PATRICK J.Capítulo IPEN-doc 02923 Comparative studies of antisera against different toxins1996 - NASCIMENTO, N.; SPENCER, P.J.; PAULA, R.A.; ANDRADE JUNIOR, H.F.; ROGERO, J.R.