PATRICK JACK SPENCER

Resumo

Possui graduação em Ciências Biológicas pela Universidade Presbiteriana Mackenzie (1991), mestrado em Tecnologia Nuclear pela Universidade de São Paulo (1995) e doutorado em Tecnologia Nuclear pela Universidade de São Paulo (2000) tendo sido bolsista sandwich no US Army Medical Research Institute for Infeccious Diseases (98-99). É responsável pelo Biotério de criação e manutenção de animais de laboratório do IPEN. Tem experiência na área de Bioquímica, com ênfase em Proteínas, atuando principalmente nos seguintes temas: veneno, proteínas, bothrops, irradiação e miotoxina.(Texto extraído do Currículo Lattes em 22 dez. 2021)

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Agora exibindo 1 - 4 de 4
  • Artigo IPEN-doc 27113
    Molecular model of cytotoxin-1 from Naja mossambica mossambica venom in complex with chymotrypsin
    2015 - MUNAWAR, AISHA; AKREM, AHMED; HUSSAIN, ASHIQ; SPENCER, PATRICK; BETZEL, CHRISTIAN
    Snake venom is a myriad of biologically active proteins and peptides. Three finger toxins are highly conserved in their molecular structure, but interestingly possess diverse biological functions. During the course of evolution the introduction of subtle mutations in loop regions and slight variations in the three dimensional structure, has resulted in their functional versatility. Cytotoxin-1 (UniProt ID: P01467), isolated from Naja mossambica mossambica, showed the potential to inhibit chymotrypsin and the chymotryptic activity of the 20S proteasome. In the present work we describe a molecular model of cytotoxin- 1 in complex with chymotrypsin, prepared by the online server ClusPro. Analysis of the molecular model shows that Cytotoxin-1 (P01467) binds to chymotrypsin through its loop I located near the N-terminus. The concave side of loop I of the toxin fits well in the substrate binding pocket of the protease. We propose Phe10 as the dedicated P1 site of the ligand. Being a potent inhibitor of the 20S proteasome, cytotoxin-1 (P01467) can serve as a potential antitumor agent. Already snake venom cytotoxins have been investigated for their ability as an anticancer agent. The molecular model of cytotoxin-1 in complex with chymotrypsin provides important information towards understanding the complex formation.
  • Artigo IPEN-doc 21327
    Venomics of the Australian eastern brown snake (Pseudonaja textilis): Detection of new venom proteins and splicing variants
    2015 - VIALA, VINCENT L.; HILDEBRAND, DIANA; TRUSCH, MARIA; FUCASE, TAMARA M.; SCIANI, JULIANA M.; PIMENTA, DANIEL C.; ARNI, RAGHUVIR K.; SCHLUTER, HARTMUT; BETZEL, CHRISTIAN; MIRTSCHIM, PETER; DUNSTAN, NATHAN; SPENCER, PATRICK J.
  • Artigo IPEN-doc 20303
    Pseudechis guttatus venom proteome: Insights into evolution and toxin clustering
    2014 - VIALA, VINCENT L.; HILDEBRAND, DIANA; TRUSCH, MARIA; ARNI, RAGHUVIR K.; PIMENTA, DANIEL C.; SCHLUTER, HARTMUT; BETZEL, CHRISTIAN; SPENCER, PATRICK J.
  • Artigo IPEN-doc 18331
    Purification, crystallization and preliminary X-ray diffraction analysis of crotamine, a myotoxic polypeptide from the Brazilian snake Crotalus durissus terrificus
    2012 - CORONADO, MONIKA A.; GEORGIEVA, DESSISLAVA; BUCK, FRIEDRICH; GABDOULKHAKOV, AZAT H.; ULLAH, ANWAR; SPENCER, PATRICK J.; ARNI, RAGHUVIR K.; BETZEL, CHRISTIAN