PATRICK JACK SPENCER

Resumo

Possui graduação em Ciências Biológicas pela Universidade Presbiteriana Mackenzie (1991), mestrado em Tecnologia Nuclear pela Universidade de São Paulo (1995) e doutorado em Tecnologia Nuclear pela Universidade de São Paulo (2000) tendo sido bolsista sandwich no US Army Medical Research Institute for Infeccious Diseases (98-99). É responsável pelo Biotério de criação e manutenção de animais de laboratório do IPEN. Tem experiência na área de Bioquímica, com ênfase em Proteínas, atuando principalmente nos seguintes temas: veneno, proteínas, bothrops, irradiação e miotoxina.(Texto extraído do Currículo Lattes em 22 dez. 2021)

Projetos de Pesquisa
Unidades Organizacionais
Cargo

Resultados de Busca

Agora exibindo 1 - 2 de 2
  • Resumo IPEN-doc 27445
    Hypanus americanus mucus
    2020 - COELHO, GUILHERME R.; PREZOTTO NETO, JOSE P.; BARBOSA, FERNANDA C.; SANTOS, RAFAEL S.; BRIGATTE, PATRICIA; SPENCER, PATRICK J.; SAMPAIO, SANDRA C.; D'AMELIO, FERNANDA; PIMENTA, DANIEL C.; SCIANI, JULIANA M.
    Fish skin plays important biological roles, such as the control of the osmotic pressure gradient, protection against mechanical forces and microorganism infections. The mucus, on the other hand, is a rich and complex fluid, important for the fish acting as innate immunity system, swimming and nutrition. The elasmobranch epidermis is characterized mainly by mucus secretory cells, and marine stingrays have already been described to present secretory glands spread throughout the body. Little is known about the biochemical composition of the stingray mucus, but recent studies denoted the importance of mucus in the envenomation process. Stingrays venom are largely studied due the human medical importance of envenoming caused by sting puncture, that evolve with local inflammation and necrosis, and these toxic events can be correlated to the chemical composition of the sting skin, according to the literature. Aiming to analyse the mucus composition, a new non-invasive mucus collection method was developed that focused on peptides and proteins, and biological assays were performed to analyze preliminary toxic and immune activities of the Hypanus americanus mucus. Pathophysiological characterization showed the presence of peptidases on mucus, as well that the induction of edema and leukocyte recruitment in mice. The fractionated mucus improved phagocytosis on macrophages and showed antimicrobial activity against T. rubrum, C. neoformans and C. albicans in vitro. The proteomic analyses showed the presence of immune-related proteins like actin, histones, hemoglobin, and ribosomal proteins. This protein pattern is similar to those reported for other fish mucus and stingray venom. This is the first report depicting the Hypanus stingray mucus composition, highlighting its biochemical composition and importance for the stingray immune system and the possible role on the envenomation process.
  • Artigo IPEN-doc 21288
    Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: Evidence of a broad arsenal of toxins
    2015 - VIALA, VINCENT L.; HILDEBRAND, DIANA; FUCASE, TAMARA M.; SCIANI, JULIANA M.; PREZOTTO NETO, JOSE P.; RIEDNER, MARIA; SANCHES, LEONARDO; NISHIMURA, PAULA J.; OGUIURA, NANCY; PIMENTA, DANIEL C.; SCHLUTER, HARTMUT; BETZEL, CHRISTIAN; ARNI, RAGHUVIR K.; SPENCER, PATRICK J.