PATRICK JACK SPENCER

Resumo

Possui graduaĆ§Ć£o em CiĆŖncias BiolĆ³gicas pela Universidade Presbiteriana Mackenzie (1991), mestrado em Tecnologia Nuclear pela Universidade de SĆ£o Paulo (1995) e doutorado em Tecnologia Nuclear pela Universidade de SĆ£o Paulo (2000) tendo sido bolsista sandwich no US Army Medical Research Institute for Infeccious Diseases (98-99). Ɖ responsĆ”vel pelo BiotĆ©rio de criaĆ§Ć£o e manutenĆ§Ć£o de animais de laboratĆ³rio do IPEN. Tem experiĆŖncia na Ć”rea de BioquĆ­mica, com ĆŖnfase em ProteĆ­nas, atuando principalmente nos seguintes temas: veneno, proteĆ­nas, bothrops, irradiaĆ§Ć£o e miotoxina.(Texto extraĆ­do do CurrĆ­culo Lattes em 22 dez. 2021)

Projetos de Pesquisa
Unidades Organizacionais
Cargo

Resultados de Busca

Agora exibindo 1 - 1 de 1
  • Artigo IPEN-doc 23048
    Protein profile analysis of two australian snake venoms by one- dimensional gel electrophoresis and MS/MS experiments
    2017 - GEORGIEVA, DESSISLAVA; HILDEBRAND, DIANA; SIMAS, RODRIGO; CORONADO, MONIKA A.; KWIATKOWSKI, MARCEL; SCHLUTER, HARTMUT; ARNI, RAGHUVIR; SPENCER, PATRICK; BETZEL, CHRISTIAN
    The Pseudechis colletti and Pseudechis butleri venoms were analyzed by 1-D gel electrophoresis, followed by mass spectrometric analysis of tryptic peptides obtained from the protein bands. Both venoms contain highly potent pharmacologically active components, which were assigned to the following protein families: basic and acidic phospholipases A2 (PLA2s), L-amino acid oxidases (LAAOs), P-III metalloproteinases (P-III SVMPs), 5ā€™- nucleotidases (5ā€™-NTDs), cysteine-rich secretory proteins (CRISPs), venom nerve growth factors (VNGFs) and post-synaptic neurotoxins. Considerable predominance of PLA2s over other toxins is a characteristic feature of both venoms. The major differences in the venom compositions are the higher concentration of SVMPs and CRISPs in the P. butleri venom, as well as the presence of post-synaptic neurotoxins. Furthermore, the analysis revealed a high concentration of proteins with myotoxic, coagulopathic and apoptotic activities. PLA2s are responsible for the myotoxic and anticoagulant effects observed in patients after envenomation (4). The other protein families, encountered in the two venoms, probably contribute to the major symptoms described for these venoms. These results explain the observed clinical effects of the black snake envenomation. The analyzed venoms contain group P-III metalloproteinases of medical importance with the potency to be used for diagnostic purposes of von Willebrand factor (vWF) disease, for regulation of vWF in thrombosis and haemostasis, for studying the function of the complement system in host defense and in the pathogenesis of diseases. Comparison of venomic data showed similarities in the major venom components of snakes from the genus Pseudechis, resulting in common clinical effects of envenomation, and demonstrating close relationships between venom toxins of Elapidae snakes.