PATRICK JACK SPENCER

Resumo

Possui graduaĆ§Ć£o em CiĆŖncias BiolĆ³gicas pela Universidade Presbiteriana Mackenzie (1991), mestrado em Tecnologia Nuclear pela Universidade de SĆ£o Paulo (1995) e doutorado em Tecnologia Nuclear pela Universidade de SĆ£o Paulo (2000) tendo sido bolsista sandwich no US Army Medical Research Institute for Infeccious Diseases (98-99). Ɖ responsĆ”vel pelo BiotĆ©rio de criaĆ§Ć£o e manutenĆ§Ć£o de animais de laboratĆ³rio do IPEN. Tem experiĆŖncia na Ć”rea de BioquĆ­mica, com ĆŖnfase em ProteĆ­nas, atuando principalmente nos seguintes temas: veneno, proteĆ­nas, bothrops, irradiaĆ§Ć£o e miotoxina.(Texto extraĆ­do do CurrĆ­culo Lattes em 22 dez. 2021)

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  • Artigo IPEN-doc 29948
    Comparing traditional and toxin-oriented approaches towards antivenom production against Bitis arietans snake venom
    2023 - GUIDOLIN, FELIPE R.; GODOI, KEMILY S. de; MEGALE, ANGELA A.A.; SILVA, CRISTIANE C.F. da; KODAMA, ROBERTO T.; CAJADO-CARVALHO, DANIELA; IWAI, LEO K.; SPENCER, PATRICK J.; PORTARO, FERNANDA C.V.; SILVA, WILMAR D. da
    Accidents with snakes are responsible for about 32,000 deaths annually in sub-Saharan Africa, caused mostly by snakes from the genus Bitis, in particular Bitis arietans. B. arietans venom is composed of a complex mixture of toxins, mainly metalloproteases, serine proteases, phospholipases, lectins, and disintegrins. In this work, we compared two approaches to anti-B. arietans antivenom production: immunization with crude snake venom (ā€œtraditional approachā€) and immunization with selected key toxins isolated from the snake venom (ā€œtoxin orientedā€ approach). Fractions from B. arietans venom were isolated by size exclusion chromatography. Crude venom and samples containing serine proteases or metalloproteases were selected for the immunization of BALB/c mice. Anti-B. arietans and anti-serine proteases plasmas showed a similar recognition profile and higher titers and affinity than the anti-metalloproteases plasma. Cross-recognition of other Bitis venoms was observed, but with low intensity. Although the plasma of all experimental groups inhibited the enzymatic activity of B. arietans venom in vitro, in vivo protection was not achieved. Our results have shown limitations in both approaches considered. Based on this, we proposed a model of polyclonal, species-specific, monovalent antivenoms that could be used as a base to produce customizable polyvalent sera for use in sub-Saharan Africa.