PATRICK JACK SPENCER

PATRICK JACK SPENCER

(Fonte: Lattes)

Resumo

Possui graduação em Ciências Biológicas pela Universidade Presbiteriana Mackenzie (1991), mestrado em Tecnologia Nuclear pela Universidade de São Paulo (1995) e doutorado em Tecnologia Nuclear pela Universidade de São Paulo (2000) tendo sido bolsista sandwich no US Army Medical Research Institute for Infeccious Diseases (98-99). É responsável pelo Biotério de criação e manutenção de animais de laboratório do IPEN. Tem experiência na área de Bioquímica, com ênfase em Proteínas, atuando principalmente nos seguintes temas: veneno, proteínas, bothrops, irradiação e miotoxina.(Texto extraído do Currículo Lattes em 22 dez. 2021)

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Immunological properties of sup(60)Co gamma rays irradiated bothropstoxin-I
2008 - BAPTISTA, J.A.; VIEIRA, D.P.; GALISTEO JUNIOR, A.J.; CAPRONI, P.; CASARE, M.; ANDRADE JUNIOR, H.F.; SPENCER, P.J.; NASCIMENTO, N.
Effects of gamma rays on the immunogenicity (IgG types) of ovalbumin
2003 - BAPTISTA, J.A.; SPENCER, P.J.; AROEIRA, L.G.S.; CASARE, M.S.; NASCIMENTO, N.
Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with gamma rays, yielding toxoids with good immunogenicity. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we used ovalbumin as a model to investigate possible immunogenic differences between native and irradiated proteins. Native ovalbumin (2mg/ml in 150mM NaCl) was irradiated with 2kGy of 60Co gamma rays with a 570Gy/h dose rate. B10.PL mice (n=5) were then immunized with either the native or the irradiated protein. After three immunizations, serum samples were collected and the antibody titers and isotypes were determined by enzyme-linked immunoadsorbant assay. Our data indicate that no difference could be noticed when the antibody titers of the two groups were compared. However, the isotyping assays indicates that the native protein induced high levels of IgG1, while its irradiated counterpart displayed mostly IgG2b antibodies. These data suggest that after irradiation, an antigen known to induce a Th2 response, is able to switch the immune system towards a Th1 pattern.
Effects of sup(60)Co radiation on the molecular structure of crotamine
2003 - CASARE, M.S.; BAPTISTA, J.A.; SPENCER, P.J.; NASCIMENTO, N.
Effects of gamma rays on the immunogenicity (IgG types) of ovalbumin
2004 - BAPTISTA, J.A.; SPENCER, P.J.; AROEIRA, L.G.S.; CASARE, M.S.; NASCIMENTO, N.
Effects of sup(60)Co radiation on the molecular structure of crotamine
2004 - CASARE, M.S.; BAPTISTA, J.A.; SPENCER, P.J.; NASCIMENTO, N.
Immunological properties of sup(60)Co gamma-rays irradiated bothropstoxin-I
2009 - BAPTISTA, J.A.; VIEIRA, D.P.; GALISTEO JUNIOR, A.J.; CAPRONI, P.; CASARE, M.; ANDRADE JUNIOR, H.F. de; SPENCER, P.J.; NASCIMENTO, N.