NANCI DO NASCIMENTO
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Artigo IPEN-doc 14485 Effect of gamma radiation on the structural and biological properties of angiotensin II2008 - NARDI, DANIELA T.; CASARE, MURILO S.; TEIXEIRA, LUIS G.D.; NASCIMENTO, NANCI; NAKAIE, CLOVIS R.Artigo IPEN-doc 14476 Young ovine death during hyperimmunization: crotalic envenomation or copper toxicosis?2008 - FERREIRA JUNIOR, R.S.; NASCIMENTO, N.; COUTO, R.; ALVES, J.B.; MEIRA, D.A.; BARRAVIERA, B.Artigo IPEN-doc 13015 Laboratory evaluation of young ovines inoculated with natural or sup(60)Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process2006 - FERREIRA JUNIOR, R.S.; NASCIMENTO, N.; COUTO, R.; ALVES, J.B.; MEIRA, D.A.; BARRAVIERA, B.Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption.Artigo IPEN-doc 11621 Study of gamma-radiation effects on crotamine and crotoxin2006 - CASARE, M.S.; SPENCER, P.; CAMPOS, L.A.; NASCIMENTO, N.Crotoxin is a 23 kDa neurotoxin from Crotalus durissus terrificus and is composed of a 9 kDa acidic subunit (crotapotin) and a 14 kDa basic subunit (phospholipase A2). Crotamine is 4882 Da, basic polypeptide with myotoxic activity. These toxins, when submitted to gamma-rays, in aqueous solution, present structural modifications, preserving their immunogenic properties. In the present work, we investigated some structural modifications on both crotoxin and crotamine after gamma-radiation using various doses, in the presence or not of "scavenger" substances. Our results indicate that irradiation leads to progressive changes in the structure of the toxin, which could explain the lower toxicity observed.Artigo IPEN-doc 14083 Study of irradiated bothropstoxin-1 with sup(6)Co gamma rays: immune system behavior2009 - CAPRONI, P.; BAPTISTA, J.A.; ALMEIDA, T.L. de; PASSOS, L.A.C.; NASCIMENTO, N.Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with 60Co gamma rays, yielding toxoids with good immunogenicity. The achievement of modified antigens with lower toxicity and preserved or improved immunogenicity can be very useful. Ionizing radiation has already been proven to be a powerful tool to attenuate snake venom toxicity without affecting, and even increasing, their immunogenic properties. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we investigated the immunological behavior of bothropstoxin-1, a K49 phospholipase, before and after irradiation. Structural modifications of the toxin were analyzed by SDS-PAGE. Isogenic mice were immunized with either the native or the irradiated toxin. The circulating antibodies were isotyped and titrated by ELISA. According to our data, irradiation promoted structural modifications in the toxin characterized by higher molecular weight forms of proteins (aggregates and oligomers). The results also indicated that irradiated toxins were immunogenic and antibodies elicited by them were able to recognize the native toxin in ELISA. These findings suggest that irradiation of toxic proteins can promote significant modifications in their structures; however they still retain many of the original antigenic and immunological properties of native proteins. Also, our data indicate that irradiated proteins induce higher titers of IgG2a and IgG2b, suggesting that Th1 cells are predominantly involved in the immune response.Artigo IPEN-doc 14173 Immunological properties of sup(60)Co gamma-rays irradiated bothropstoxin-I2009 - BAPTISTA, J.A.; VIEIRA, D.P.; GALISTEO JUNIOR, A.J.; CAPRONI, P.; CASARE, M.; ANDRADE JUNIOR, H.F. de; SPENCER, P.J.; NASCIMENTO, N.