NANCI DO NASCIMENTO
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Artigo IPEN-doc 20886 New insights into the structural characteristics of irradiated crotamine2015 - OLIVEIRA, KARINA C.; SPENCER, PATRICK J.; FERREIRA JUNIOR, RUI S.; NASCIMENTO, NANCIResumo IPEN-doc 13668 Immunological properties of sup(60)Co gamma rays irradiated bothropstoxin-I2008 - BAPTISTA, J.A.; VIEIRA, D.P.; GALISTEO JUNIOR, A.J.; CAPRONI, P.; CASARE, M.; ANDRADE JUNIOR, H.F.; SPENCER, P.J.; NASCIMENTO, N.Artigo IPEN-doc 10737 Immune response against bothropstoxin-I irradiated with sup(60) gamma rays2005 - BAPTISTA, J.A.; SPENCER, P.J.; HIGA, O.Z.; ANDRADE JUNIOR, H.F.; CESARE, M.S.; CAMPOS, L.A.; NASCIMENTO, N.Artigo IPEN-doc 19269 Structural analysis of irradiated crotoxin by spectroscopic techniques2013 - OLIVEIRA, KARINA C. de; FUCASE, TAMARA M.; SILVA, ED CARLOS S.E.; CHAGAS, BRUNO B.; BUCHI, ALISSON T.; VIALA, VINCENT L.; SPENCER, PATRICK J.; NASCIMENTO, NANCI doArtigo IPEN-doc 15163 Evaluation of moniotoxic activity of bothropstoxin-1 irradiated with sup(60)Co gamma rays2009 - MIRCO, JESSICA; BAPTISTA, JANAINA A.; CAPRONI, PRISCILA; YOSHITO, DANIELE; NASCIMENTO, NANCIIonizing radiation has been successfully employed to modify the immunological properties of biomolecules and has been proven to be a powerful tool to attenuate snake venoms toxicity without affecting their immunogenic properties. Snake venoms and their isolated toxins showed structural modifications after gamma radiation process, in aqueous solution, mainly by water radiolysis sub products. Free radical scavengers, such as NaNO3 and t-butanol, present selective effects on specific radical from water radiolysis. The NaNO3 has affinity by aqueous electron, while the t-butanol has affinity by hydroxyl radical. At the present work, we have investigated the miotoxic activity of bothropstoxin-1 (BTHX-1), a K49 phospholipase, present in Bothrops jararacussu crude venom, before and after irradiation process, with or without scavenger substances presence. BTHX-1 was irradiated with 2kGy of 60Co gamma rays, in aqueous solution and in the presence of oxygen. BALB/c mice were inoculated with either native or irradiated toxin, with or without scavenger substances. After 3 hours, blood samples were collected and the miotoxic activity was evaluated by LDH (lactate dehydrogenase) release. The muscular tissue damage was directly related to the LDH amounts released. Irradiated bothropstoxin-1, with or without NaNO3 substance, caused less damage than their native counterpart. But irradiated toxin, in the presence of t-butanol, was so miotoxic as the native BTHX-1. These results indicate that irradiation of toxic proteins can promote significant modifications on their structures, but still retaining many of the original biological properties of their native counterparts. Additionally, some scavengers substances can change these gamma radiation effects.Artigo IPEN-doc 15156 Physico-chemical characterization of gamma rays irradiated crotamine2009 - OLIVEIRA, KARINA C. de; SPENCER, PATRICK J.; NASCIMENTO, NANCIIonizing radiation can change the molecular structure and affect the biological properties of biomolecules. It has been employed to attenuate animal toxins. Crotamine, a toxin from Crotalus durissus terrificus (Cdt), is a highly basic polypeptide (pI - 10.3), with myotoxic activity and molecular weight of 4882 Da. It is composed of 42 amino acids residues and reticulated by three disulfide bonds. This study aimed the characterization of irradiated crotamine using Circular Dichroism (CD), Fluorescence Spectroscopy and Differential Scanning Calorimetry (DSC) techniques. We used size exclusion and ion-exchange chromatography to purify it from Cdt crude venom. The pure crotamine was irradiated with 2.0 kGy from a 60Co source. Native and irradiated crotamine were analyzed in a fluorescence spectrophotometer (Hitachi F-4500), under excitation wavelength at 275 nm and the emission was scanned from 300 to 500 nm. The analysis of fluorescence quenching showed that the irradiated form displayed a lower quantum yield when compared to the native form. CD spectra, obtained from a Jasco, J-180 spectropolarimeter, of native and irradiated crotamine solutions, showed a discrete change between the samples, from apparently ordered conformation to a random coil. Finally, the thermodynamics analysis, realized in a calorimeter METTLER TOLEDO, DSC 822e, showed that irradiation promoted changes in the calorimetric profile. Our results indicate that irradiation leads to progressive changes in the structure of the toxin, which could explain the decrease in myotoxic activity.Resumo IPEN-doc 13117 The lesson from snake venom: ion transport and glycolysis are couple through the pumping rate of the Na/KAT pase2007 - LIMA, V.M.F. de; HANKE, W.; CAMILLO, M.A.; SPENCER, P.; NASCIMENTO, N.Artigo IPEN-doc 14485 Effect of gamma radiation on the structural and biological properties of angiotensin II2008 - NARDI, DANIELA T.; CASARE, MURILO S.; TEIXEIRA, LUIS G.D.; NASCIMENTO, NANCI; NAKAIE, CLOVIS R.Artigo IPEN-doc 14476 Young ovine death during hyperimmunization: crotalic envenomation or copper toxicosis?2008 - FERREIRA JUNIOR, R.S.; NASCIMENTO, N.; COUTO, R.; ALVES, J.B.; MEIRA, D.A.; BARRAVIERA, B.Artigo IPEN-doc 13015 Laboratory evaluation of young ovines inoculated with natural or sup(60)Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process2006 - FERREIRA JUNIOR, R.S.; NASCIMENTO, N.; COUTO, R.; ALVES, J.B.; MEIRA, D.A.; BARRAVIERA, B.Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption.