NANCI DO NASCIMENTO
9 resultados
Resultados de Busca
Agora exibindo 1 - 9 de 9
Resumo IPEN-doc 13668 Immunological properties of sup(60)Co gamma rays irradiated bothropstoxin-I2008 - BAPTISTA, J.A.; VIEIRA, D.P.; GALISTEO JUNIOR, A.J.; CAPRONI, P.; CASARE, M.; ANDRADE JUNIOR, H.F.; SPENCER, P.J.; NASCIMENTO, N.Artigo IPEN-doc 10787 Metacyclic promastigotes of Leishmania amazonensis selection using gamma irradiation2005 - BONETTI, F.C.; ANDRADE JUNIOR, H.F.; NASCIMENTO, N.Leishmania spp. causes a spectrum of human diseases, ranging from self-healing skin lesions to severe and lethal visceral disease. In previous work we demonstrated that the protein and nucleic acid metabolism and oxidative respiration were severely affected by irradiation, in a dose response way, but a small but representative fractions are relatively radio resistant, surviving after 800 Gy of 60Co irradiation. The best explanation could be a selection of metacyclic promastigotes. In these forms, the G0 state allows the adequate correction of DNA repair after the irradiation insult. In this work, we are looking for the ideal radiation dose to select the higher proportion of metacyclic forms of L.. (L.) amazonensis in culture. Parasites were grown in RPMI 1640 medium, plus 20% fetal calf serum, than they were irradiated with differents doses rangin between 25 and 400 Gy. Parasites irradiated at 400 Gy infected, proportionally, more cells than parasites irradiated at other doses. To confirm this metacyclogenesis, a complement lysis assay was performed with 5, 10 and 20% of male guinea pig blood serum at 20ºC for 3 hours, and parasites counted. Guinea pig serum a 10% promotes more lysis, with 200 Gy irradiated parasites being less affected, probably due to metacyclic selection. These preliminary results suggests that the ionizing radiation, specially between 200 and 400 Gy, could be a alternative tool for the selection of metacyclic forms of Leishmania amazonensis in culture.Artigo IPEN-doc 10726 Influence of ionizing radiation on trypanosoma cruzi2005 - SZAROTA, R.M.; ANDRADE JUNIOR, H.F.; BAPTISTA, J.A.; DIAS, V.L.; GIMENES, A.P.; MARTINS, A.R.S.; NASCIMENTO, N.; PASSOS, L.A.C.Artigo IPEN-doc 10737 Immune response against bothropstoxin-I irradiated with sup(60) gamma rays2005 - BAPTISTA, J.A.; SPENCER, P.J.; HIGA, O.Z.; ANDRADE JUNIOR, H.F.; CESARE, M.S.; CAMPOS, L.A.; NASCIMENTO, N.Artigo IPEN-doc 15116 Tissue distribution of radiolabeled phosphatidylserine containing liposome in mice2009 - BORBOREMA, SAMANTA E.T.; ANDRADE JUNIOR, HEITOR F.; OSSO JUNIOR, JOAO A.; NASCIMENTO, NANCIArtigo IPEN-doc 11954 Humoral immune response of C57Bl/6j and BALB/c mice immunized with irradiated techyzoites of Toxoplasma gondii RH strain and oral challenge with Me-49 strain2007 - GALISTEO JUNIOR, ANDRES J.; ZORGI, NAHIARA E.; ALVES, JANAINA B.; HIRAMOTO, ROBERTO M.; NASCIMENTO, NANCI do; ANDRADE JUNIOR, HEITOR F. deArtigo IPEN-doc 11952 Structure alteration and immunological properties of sup(60)Co gamma rays irradiated bothropstoxin-I2007 - BAPTISTA, JANAINA A.; VIEIRA, DANIEL P.; GALISTEO JUNIOR, ANDRES J.; YONAMINE, CAMILA M.; CAPRONI, PRISCILA; CASARE, MURILO; ANDRADE JUNIOR, HEITOR F. de; SPENCER, PATRICK J.; NASCIMENTO, NANCI doArtigo IPEN-doc 11941 Pharmacokinetic of antimony in mice with cutaneous leishmaniasis2007 - BORBOREMA, SAMANTA E.T.; ANDRADE JUNIOR, HEITOR F. de; OSSO JUNIOR, JOAO A.; NASCIMENTO, NANCI doArtigo IPEN-doc 14173 Immunological properties of sup(60)Co gamma-rays irradiated bothropstoxin-I2009 - BAPTISTA, J.A.; VIEIRA, D.P.; GALISTEO JUNIOR, A.J.; CAPRONI, P.; CASARE, M.; ANDRADE JUNIOR, H.F. de; SPENCER, P.J.; NASCIMENTO, N.