DIAS, CARLA R.OSSO JUNIOR, JOAO A.2014-11-172014-11-182015-04-022014-11-172014-11-182015-04-02DIAS, CARLA R.; OSSO JUNIOR, JOAO A. Radiolabeling of anti-cd20 with Re-188: liquid kit. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 8th/ MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 15th, Sept. 30 - Oct. 5, 2007, Santos, SP. <b>Proceedings...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/18110.http://repositorio.ipen.br/handle/123456789/18110Radioimmunotherapy uses the targeting features of monoclonal antibody to deliver radiation from an attached radionuclide. The radionuclide 188Re is currently produced from the father nuclide 188W through a transportable generator system. Because of its easy availability and suitable nuclear properties (EβMAX =2.1 MeV, t1/2=16.9 h, Eγ=155 keV), this radionuclide is considered an attractive candidate for application as therapeutic agents and could be conveniently utilized for imaging and dosimetric purposes. The objective of this work is the optimization of radiolabeling of anti-CD20 with 188Re using a liquid formulation. Anti-CD20 was reduced by incubation with 2-ME and purified over a PD-10 column. The number of resulting free SH was assayed with Ellman’s reagent. Optimization of radiolabeling was achieved by varying parameters: antibody mass, reducing agent, reaction time and 188Re volume in the liquid kit. Radiochemical purity of 188Re-anti-CD20 was evaluated. An average of 12 SH groups per mol in the reductions was found. The best labeling efficiency (> 93%) was achieved in the following conditions: 1 mg anti-CD20; 82.8 mg sodium tartrate; 1 mg SnCl2; 0.25 mg gentisic acid, 1 mL 188Re and reaction time of 1 hour at room temperature.openAccessactivity levelselutriationexperimental dataimpuritiesion exchangeisotope productionlutetium 176lutetium 177nuclear reactionsytterbium 175ytterbium 176 targetytterbium 177Texto completo de eventohttps://orcid.org/0000-0002-6672-1631