PRETTO, LUCAS R. deMOULT, ERIC M.ALIBHAI, A.Y.CARRASCO-ZEVALLOS, OSCAR M.CHEN, SIYULEE, BYUNGKUNWITKIN, ANDRE J.BAUMAL, CAROLINE R.REICHEL, ELIASFREITAS, ANDERSON Z. deDUKER, JAY S.WAHEED, NADIA K.FUJIMOTO, JAMES G.2019-11-292019-11-292019PRETTO, LUCAS R. de; MOULT, ERIC M.; ALIBHAI, A.Y.; CARRASCO-ZEVALLOS, OSCAR M.; CHEN, SIYU; LEE, BYUNGKUN; WITKIN, ANDRE J.; BAUMAL, CAROLINE R.; REICHEL, ELIAS; FREITAS, ANDERSON Z. de; DUKER, JAY S.; WAHEED, NADIA K.; FUJIMOTO, JAMES G. Controlling for artifacts in widefield optical coherence tomography angiography measurements of non-perfusion area. <b>Scientific Reports</b>, v. 9, p. 1-15, 2019. DOI: <a href="https://dx.doi.org/10.1038/s41598-019-43958-1">10.1038/s41598-019-43958-1</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/30388.2045-2322http://repositorio.ipen.br/handle/123456789/30388The recent clinical adoption of optical coherence tomography (OCT) angiography (OCTA) has enabled non-invasive, volumetric visualization of ocular vasculature at micron-scale resolutions. Initially limited to 3 mm × 3 mm and 6 mm × 6 mm fields-of-view (FOV), commercial OCTA systems now offer 12 mm × 12 mm, or larger, imaging fields. While larger FOVs promise a more complete visualization of retinal disease, they also introduce new challenges to the accurate and reliable interpretation of OCTA data. In particular, because of vignetting, wide-field imaging increases occurrence of low-OCT-signal artifacts, which leads to thresholding and/or segmentation artifacts, complicating OCTA analysis. This study presents theoretical and case-based descriptions of the causes and effects of low-OCTsignal artifacts. Through these descriptions, we demonstrate that OCTA data interpretation can be ambiguous if performed without consulting corresponding OCT data. Furthermore, using wide-field non-perfusion analysis in diabetic retinopathy as a model widefield OCTA usage-case, we show how qualitative and quantitative analysis can be confounded by low-OCT-signal artifacts. Based on these results, we suggest methods and best-practices for preventing and managing low-OCT-signal artifacts, thereby reducing errors in OCTA quantitative analysis of non-perfusion and improving reproducibility. These methods promise to be especially important for longitudinal studies detecting progression and response to therapy.1-15openAccessophthalmologyretinabiomedical radiographytomographyvascular diseasesoptical equipmentcoherent radiationimagesblood vesselsbeam scannersArtigo de periódico910.1038/s41598-019-43958-10000-0002-5018-9126https://orcid.org/0000-0002-5018-912676.76193.00