BARBEZAN, ANGELICA B.SANTOS, CARLA J.B.CARVALHO, LUMA R.VIEIRA, DANIEL P.SANTELLI, GLAUCIA M.M.VILLAVICENCIO, ANNA L.C.H.2019-07-312019-07-312019BARBEZAN, ANGELICA B.; SANTOS, CARLA J.B.; CARVALHO, LUMA R.; VIEIRA, DANIEL P.; SANTELLI, GLAUCIA M.M.; VILLAVICENCIO, ANNA L.C.H. Flow cytometry based micronucleus assay for evaluation of genotoxic potential of 2-ACBs in hepatic cells HepG2. <b>Brazilian Journal of Radiation Sciences</b>, v. 7, n. 2A, p. 1-24, 2019. DOI: <a href="https://dx.doi.org/10.15392/bjrs.v7i2A.684">10.15392/bjrs.v7i2A.684</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/29987.2319-0612http://repositorio.ipen.br/handle/123456789/29987Food irradiation is approved for use in more than 60 countries for applications and purposes in a wide variety of foods, being an effective and safe method for preservation and long-term storage. 2- Alkylcyclobutanones (2-ACBs) are the only known radiolytic products generated from foods that contain fatty acids (Triglycerides) when irradiated. The acids analyzed in this study are palmitic and stearic, which when irradiated form 2-Dodecylcyclobutanones (2-dDCB) and 2-Tetradecylcyclobutanone (2-tDCB). Part of the 2-ACBs ingested is excreted through feces and part is deposited in adipose tissues. In vitro studies so far have been only in colon cells. The work used a human hepatoma cell line (HepG2) since the accumulation of fat in this organ is quite common. Micronucleus test was selected to evaluate possible genotoxic effects of 2-dDCB and 2-tDCB compounds when exposed to high concentrations (447, 1422 and 2235 μM) for 4 and 24 hours. Tests were performed in quadriplicates using flow cytometric analysis. None detectable genotoxic damage was observed after 4 hours of exposure to the compounds, and cytotoxic effects were only significant at the highest concentration (2235 μM) of 2-dDCB. After 24 hours of exposure, slight genotoxic damage was observed at all concentrations evaluated, and cytotoxic effects were only present when exposed to compound 2-tDCB. Although there is a genotoxic and cytotoxic effect in some of the situations tested, the two compounds predominantly induced proliferation reduction effects of this hepatic tumor cell line.1-24openAccessfood processingirradiationhexadecanoic acidcell flow systemsdodecyl radicalshepatomasin vitrooctadecanoic acidradiation effectstoxicitycarcinomasArtigo de periódico2A710.15392/bjrs.v7i2A.6840000-0001-6199-78770000-0002-0007-534Xhttps://orcid.org/0000-0001-6199-7877https://orcid.org/0000-0002-0007-534XSem Percentil