ISAAC, CESARMATHOR, MONICA B.BARIANI, GIOVANIPAGGIARO, ANDRE O.HERSON, MARISA R.SCHAINBERG, CLÁUDIA G.CARRASCO, SOLANGETEODORO, WALCY R.YOSHINARI, NATALINO H.FERREIRA, MARCUS C.2014-07-152014-07-302014-07-152014-07-302009ISAAC, CESAR; MATHOR, MONICA B.; BARIANI, GIOVANI; PAGGIARO, ANDRE O.; HERSON, MARISA R.; SCHAINBERG, CLÁUDIA G.; CARRASCO, SOLANGE; TEODORO, WALCY R.; YOSHINARI, NATALINO H.; FERREIRA, MARCUS C. Pentoxifylline modifies three-dimensional collagen lattice model contraction and expression of collagen types I and III by human fibroblasts derived from post-burn hypertrophic scars and from normal skin. <b>Burns</b>, v. 35, n. 5, p. 701-706, 2009. DOI: <a href="https://dx.doi.org/10.1016/j.burns.2008.11.017">10.1016/j.burns.2008.11.017</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/4839.0305-4179http://repositorio.ipen.br/handle/123456789/4839Fibroblasts are thought to be partially responsible for the persisting contractile forces that result in burn contractures. Using a monolayer cell culture and fibroblast populated collagen lattice (FPCL) three-dimensional model we subjected hypertrophic scar and non-cicatricial fibroblasts to the antifibrogenic agent pentoxifylline (PTF – 1 mg/mL) in order to reduce proliferation, collagen types I and III synthesis and model contraction. Fibroblasts were isolated from post-burn hypertrophic scars (HSHF) and non-scarred skin (NHF). Cells were grown in monolayers or incorporated into FPCL's and exposed to PTF. In monolayer, cell number proliferation was reduced (46.35% in HSHF group and 37.73% in NHF group, p < 0.0001). PTF selectively inhibited collagen III synthesis in the HSHF group while inhibition was more evident to type I collagen synthesis in the NHF group. PTF also reduced contraction in both (HSHF and NHF) FPCL.701-706openAccesscell culturesfibroblastscollagenthree-dimensional calculationshypertrophyskin diseasesArtigo de periódico53510.1016/j.burns.2008.11.017http://orcid.org/0000-0002-7294-9106