SANTOS, ELEN G.TOMIYAMA, CLAUDIA S.KODAIRA, CLAUDIA A.LOURENCO, ANA V.S.FELINTO, MARIA C.F.C.BRITO, HERMI F.BRITO, MARIA E.F.2014-11-172014-11-182015-04-022014-11-172014-11-182015-04-02SANTOS, ELEN G.; TOMIYAMA, CLAUDIA S.; KODAIRA, CLAUDIA A.; LOURENCO, ANA V.S.; FELINTO, MARIA C.F.C.; BRITO, HERMI F.; BRITO, MARIA E.F. Using lanthanide chelates and uranyl compounds for diagnostic by fluoroimmunoassays. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 9th; MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 16th; MEETING ON NUCLEAR INDUSTRY, 1st, September 27 - October 2, 2009, Rio de Janeiro, RJ. <b>Proceedings...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/16547.http://repositorio.ipen.br/handle/123456789/16547The importance of the luminescence of lanthanide ions and UO2 2+ is related to its peculiar characteristics, e.g. long lifetime and line-like emission bands in the visible, which make these ions unique among the species that are known to luminescence. Recent developments in the field of supramolecular chemistry have allowed the design of ligands capable of encapsulating lanthanide ions, thus forming kinetically inert complexes. By introduction of chromophoric groups in these ligands, an intense luminescence of the ion can be obtained via the "antenna effect", defined as a light conversion process involving distinct absorbing (ligand) and emitting (metal ion) components. In such a process, the quantities that contribute to the luminescence intensity are (i) the efficiency of the absorption, (ii) the efficiency of the ligand-metal energy transfer, and (iii) the efficiency of the metal luminescence. Encapsulation of lanthanide ions with suitable ligands may therefore give rise to "molecular devices" capable to emit strong, long-lived luminescence. Besides the intrinsic interest in their excited state properties, compounds of lanthanide ions, in particular of the Eu3+ and Tb3+ ions, and now UO2 2+ are important for their potential use as luminescent labels for biological species in fluoroimmunoassays (FIAs). This is most interesting because fluorimetric labeling represents an alternative method to the use of radioactive labels, which has long been the most common way of quantifying immunoreactions. In this article we report information about luminescent materials, which gave a good signal to quantify biological molecules by TR-FIA, DELFIA , DSLFIA, RIA and FRET.openAccesschelatesefficiencyenergy transfereuropium ionsimmunoassaylabellingligandsluminescencerare earthsterbium ionsuranyl compoundsTexto completo de eventohttps://orcid.org/0000-0001-7028-0878