CABRAL, FERNANDA V.SABINO, CAETANO P.DIMMER, JESICA A.SAUTER, ISMAEL P.CORTEZ, MAURO J.RIBEIRO, MARTHA S.2020-06-192020-06-192020CABRAL, FERNANDA V.; SABINO, CAETANO P.; DIMMER, JESICA A.; SAUTER, ISMAEL P.; CORTEZ, MAURO J.; RIBEIRO, MARTHA S. Preclinical investigation of methylene blue-mediated antimicrobial photodynamic therapy on Leishmania parasites using real-time bioluminescence. <b>Photochemistry and Photobiology</b>, v. 96, n. 3, p. 604-610, 2020. DOI: <a href="https://dx.doi.org/10.1111/php.13188">10.1111/php.13188</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/31330.0031-8655http://repositorio.ipen.br/handle/123456789/31330Cutaneous leishmaniasis (CL) is a neglected disease that promotes destructive lesions. Difficulties in treatment are related to accessibility of drugs, resistance and toxicity. Antimicrobial photodynamic therapy (APDT) has been emerging as a promising treatment for CL. In this work, we evaluated methylene blue (MB)-mediated APDT (MB-APDT) on Leishmania amazonensis in vitro and in vivo by bioluminescence technique. In vitro, MB-APDT was performed using a red LED (k = 660 11 nm, 100 mW cm 2) and MB (100 μM) at different light doses. In vivo, mice were infected and 4 weeks later, randomly divided into three groups: control, APDT 1 (single session) and APDT 2 (two sessions of MB-APDT). MB was used at 100 μM and energy dose was established at 150 J cm 2. Parasite burden, lesion size and pain were evaluated weekly for 4 weeks. In vitro, lethal dose for 90% parasite inactivation was achieved at 48.8 J cm 2. In vivo, although APDT 1 and APDT 2 groups have showed similar parasite burden after 4 weeks, two sessions were clinically better, especially considering the inflammatory process associated to CL. Our findings reinforce MB-APDT as a costeffective treatment to combat CL.604-610closedAccessantimicrobial agentstherapyphotosensitivitymethylene blueparasitesbioluminescencephotochemistryin vivoin vitromiceArtigo de periódico39610.1111/php.131880000-0002-4203-1134https://orcid.org/0000-0002-4203-113448.4773.00