TEIXEIRA, LUIZ F.S.PERON, JEAN P.S.BELLINI, MARIA H.2020-04-072020-04-072020TEIXEIRA, LUIZ F.S.; PERON, JEAN P.S.; BELLINI, MARIA H. Silencing of nuclear factor kappa b 1 gene expression inhibits colony formation, cell migration and invasion via the downregulation of interleukin 1 beta and matrix metallopeptidase 9 in renal cell carcinoma. <b>Molecular Biology Reports</b>, v. 47, n. 2, p. 1143-1151, 2020. DOI: <a href="https://dx.doi.org/10.1007/s11033-019-05212-9">10.1007/s11033-019-05212-9</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/31107.0301-4851http://repositorio.ipen.br/handle/123456789/31107Renal cell carcinoma (RCC) is a highly deadly urological tumor due to its high metastatic incidence and its notorious chemoresistance. The nuclear transcription factor kappa B (NF-κB) family has been associated with apoptosis resistance and cellular invasion in RCC. The purpose of this study was to evaluate the impact of NF-κB1 gene silencing on the colony formation, cell migration and invasion abilities of the RCC cell line. Renca–mock and Renca-shRNA-NF-κB1 cells were used in this work. NF-κB1 downregulation was assessed by western blotting. The mRNA expression levels of interleukin-1 beta (IL-1β) and MMP-9 were assessed by real-time quantitative polymerase chain reaction (RT-qPCR). The IL-1β levels in the culture media were determined by a commercial ELISA kit. The MMP-9 protein expression and gelatinolytic activity were evaluated by western blotting and zymography, respectively, and the migration and invasion abilities were analysed. The expression levels of p105 and p50 in Renca-shRNA-NF-κBmoc1 cells were significantly reduced compared with those in the Renca–mock cells. The colony numbers of shRNA-NF-кB1 cells were lower than the colony numbers of the Renca– mock cells. NF-κB1 knockdown inhibited the cell migration and invasion of Renca-shRNA-NF-κB1 cells. These cells also exhibited reduced levels of IL-1β. The MMP-9 expression and activity levels were significantly reduced in Renca-shRNANF- κB1 cells. Taken together, these results indicate that the downregulation of NF-κB1 suppresses the tumourigenicity of RCC by reducing MMP-9 expression and activity; thus, NF-κB1 could be a molecular target for RCC treatment.1143-1151openAccesskidneyscarcinomastranscription factorscolony formationmigrationcarcinogenesistranscriptiontranscription factorsantigensproteinsArtigo de periódico24710.1007/s11033-019-05212-9https://orcid.org/0000-0003-2852-618919.7015.00