JUNIOR, ALEXANDRE F.RIBEIRO, CHARLENE A.LEYVA, MARIA E.MARQUES, PAULO S.SOARES, CARLOS R.J.QUEIROZ, ALVARO A.A. de2022-01-272022-01-272022JUNIOR, ALEXANDRE F.; RIBEIRO, CHARLENE A.; LEYVA, MARIA E.; MARQUES, PAULO S.; SOARES, CARLOS R.J.; QUEIROZ, ALVARO A.A. de. Biophysical properties of electrospun chitosan-grafted poly(lactic acid) nanofibrous scaffolds loaded with chondroitin sulfate and silver nanoparticles. <b>Journal of Biomaterials Applications</b>, v. 36, n. 6, p. 1098-1110, 2022. DOI: <a href="https://dx.doi.org/10.1177/08853282211046418">10.1177/08853282211046418</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/32667.0885-3282http://repositorio.ipen.br/handle/123456789/32667The aim of this work was to study the biophysical properties of the chitosan-grafted poly(lactic acid) (CH-g-PLA) nanofibers loaded with silver nanoparticles (AgNPs) and chondroitin-4-sulfate (C4S). The electrospun CH-g-PLA:AgNP:C4S nanofibers were manufactured using the electrospinning technique. The microstructure of the CH-g-PLA:AgNP:C4S nanofibers was investigated by proton nuclear magnetic resonance (1H-NMR), scanning electron microscopy (SEM), UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), and Fourier transform infrared (ATR-FTIR) spectroscopy. ATR-FTIR and 1H-NMR confirm the CH grafting successfully by PLA with a substitution degree of 33.4%. The SEM measurement results indicated apparently smooth nanofibers having a diameter range of 340 ± 18 nm with porosity of 89 ± 3.08% and an average pore area of 0.27 μm2. UV-Vis and XRD suggest that silver nanoparticles with the size distribution of 30 nm were successfully incorporated into the electrospun nanofibers. The water contact angle of 12.8 ± 2.7° reveals the hydrophilic nature of the CH-g-PLA:AgNP:C4S nanofibers has been improved by C4S. The electrospun CH-g-PLA:AgNP:C4S nanofibers are found to release ions Ag+ at a concentration level capable of rendering an antimicrobial efficacy. Gram-positive bacteria (S.aureus) were more sensitive to CH-g-PLA:AgNP:C4S than Gram-negative bacteria (E. coli). The electrospun CH-g-PLA:AgNP:C4S nanofibers exhibited no cytotoxicity to the L-929 fibroblast cells, suggesting cytocompatibility. Fluorescence microscopy demonstrated that C4S promotes the adhesion and proliferation of fibroblast cells onto electrospun CH-g-PLA:AgNP:C4S nanofibers.1098-1110openAccesssilvernanoparticlesnanofiberslactic acidArtigo de periódico63610.1177/08853282211046418https://orcid.org/0000-0002-7982-178928.552