JACOVONE, R.M.COSTA, C.A.SAKATA, S.K.2021-03-052021-03-05JACOVONE, R.M.; COSTA, C.A.; SAKATA, S.K. Chemical characterization of bacteria: graphene oxide/Ag nanocomposite interactions by atomic force microscopy -infrared spectroscopy. In: PAN-AMERICAN NANOTECHNOLOGY CONFERENCE, 2nd, March 4-7, 2020, Águas de Lindoia, SP. <b>Abstract...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/31874.http://repositorio.ipen.br/handle/123456789/31874Atomic force microscopy -Infrared spectroscopy (AFM-IR) is a combined technique that allows nanoscale chemical characterization of biological–materials interactions. In this work, AFM-IR was used to map Escherichia coli in graphene oxide /silver nanocomposite (Ag/rGO). In Escherichia coli, it was observed absorption bands corresponding to amine I at 1660 cm-1 and amide II at 1550 cm-1 from proteins. On the other hand, when these bacteria were exposed to (Ag/rGO) typical absorption bands from carbonyl/carboxyl groups around 1745 and carbon bond around 1620 were also detected, showing the antibacterial activities of (Ag/rGO). The conventional atomic force microscope was used to elucidate the morphologic changes that occurred by internalization of nanocomposite into the bacteria. Atomic force microscopy -Infrared spectroscopy (AFM-IR) is a combined technique that allows nanoscale chemical characterization of biological–materials interactions. In this work, AFM-IR was used to map Escherichia coli in graphene oxide /silver nanocomposite (Ag/rGO). In Escherichia coli, it was observed absorption bands corresponding to amine I at 1660 cm-1 and amide II at 1550 cm-1 from proteins. On the other hand, when these bacteria were exposed to (Ag/rGO) typical absorption bands from carbonyl/carboxyl groups around 1745 cm-1 and carbon bond around 1620 cm-1 were also detected, showing the antibacterial activities of (Ag/rGO). The conventional atomic force microscope was used to elucidate the morphologic changes that occurred by internalization of nanocomposite into the bacteria.openAccessResumo de eventos científicos0000-0001-6072-5853https://orcid.org/0000-0001-6072-5853