QUEIROZ, ALVARO A.A. deABRAHAM, GUSTAVO A.CAMILLO, MARIA A.P.HIGA, OLGA Z.SILVA, GERALD S.FERNANDEZ, MARIA DEL M.ROMAN, JULIO S.2014-07-312014-07-312014-07-312014-07-312006QUEIROZ, ALVARO A.A. de; ABRAHAM, GUSTAVO A.; CAMILLO, MARIA A.P.; HIGA, OLGA Z.; SILVA, GERALD S.; FERNANDEZ, MARIA DEL M.; ROMAN, JULIO S. Physicochemical and antimicrobial properties of boron-complexed polyglycerol-chitosan dendrimers. <b>Journal of Biomaterials Science, Polymer Edition</b>, v. 17, n. 6, p. 689-707, 2006. DOI: <a href="https://dx.doi.org/10.1163/156856206777346313">10.1163/156856206777346313</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/8307.0920-5063http://repositorio.ipen.br/handle/123456789/8307A polyglycerol with dendritic structure (PGLD) was synthesized by ring-opening polymerization of deprotonated glycidol using a polyglycerol as core functionality in a step-growth process. Then, PGLD reacted with O-carboxymethylated chitosan to obtain PGLD-chitosan dendrimer (PGLD-Ch). After the reaction of PGLD-Ch with boric acid, there was a marked increase in the bulk viscosity evidencing physically that boron can initiate a charge transfer complex formation, (PGLD-Ch)B. Gel permeation chromatography analysis was used to characterize the molecular weight and the polydispersivity of the synthesized PGLD-Ch. A dendritic structure with a molecular mass of 16.7 kDa and a narrow polydispersity (Mw/Mn = 1.05) was obtained. 1H-NMR and 13C-NMR measurements were employed to assess the degree of branching in PGLD. The obtained value of 0.85 indicates the tendency toward a dentritic structure for PGLD. The glass transition temperature values of (PGLD-Ch)B membranes containing 10% and 30% PGLD were -19°C and -26°C, respectively, which favor its potential use as surface coating of several polymers. The in vitro cytotoxicity was evaluated using the minimum essential medium elution test assay. Extracts of boron-complexed PGLD exhibited lower cytotoxicity than the controls, suggesting that the material has an improved biocompatibility. Antibacterial studies of (PGLD-Ch)B against Staphylococcus aureus and Pseudomonas aeruginosa showed a significant activity. Our study confirms and supports the effectiveness of (PGLD-Ch)B as an antimicrobial coating due to its capacity in suppressing the bacterial proliferation. The best in vivo response was found for (PGLD-Ch)B-30 membranes, which exhibited higher synthesis of collagen fibers than PGLD-ChB-10.689-707openAccessglycerolpolysaccharidesboron complexesantimicrobial agentswoundshealingArtigo de periódico61710.1163/156856206777346313