QUITERO, MAYRA F.Z.SIRIANI, LUCIANA K.AZEVEDO, CYNTHIA S. deFREITAS, ANDERSON Z. deSCARAMUCCI, TAISSIMIONATO, MARIA R.L.MATOS, ADRIANA B.2020-03-022020-03-022019QUITERO, MAYRA F.Z.; SIRIANI, LUCIANA K.; AZEVEDO, CYNTHIA S. de; FREITAS, ANDERSON Z. de; SCARAMUCCI, TAIS; SIMIONATO, MARIA R.L.; MATOS, ADRIANA B. Optical coherence tomography and polarized light microscopy for the evaluation of artificial caries: a preliminary study. <b>General Dentistry</b>, v. 67, n. 1, p. e1-e6, 2019. Disponível em: http://repositorio.ipen.br/handle/123456789/30858.0363-6771http://repositorio.ipen.br/handle/123456789/30858This study was designed to investigate whether there is a correlation between the findings of optical coherence tomography (OCT) and polarized light microscopy (PLM) when these techniques are used to evaluate standard enamel white-spot lesions developed by distinct cariogenic challenges. Bovine enamel fragments (N = 168) were randomly allocated into 6 experimental groups according to the microbiologic model (Streptococcus mutans UA159, Streptococcus sobrinus ATCC 33478, or mixed S mutans and S sobrinus) and carbohydrate sources (1% sucrose or combined 1% sucrose and 1% starch). Specimens were examined by OCT and PLM every day within a period of 7 days. Five measurements of demineralization depth were recorded for each specimen, and means were calculated. Data were analyzed with analysis of variance and Tukey tests (α = 0.05), and a correlation test was performed. All cariogenic challenges created sub-superficial lesions. In both the OCT and PLM analyses, the demineralization depth reached its peak between days 6 and 7 of the cariogenic challenge, except for the group challenged with S sobrinus supplemented with combined sucrose and starch; for that group, demineralization peaked on day 5 in the OCT analysis. There was a significant correlation between OCT and PLM (P = 0.00; r = 0.842). This preliminary study suggests that OCT is a reliable, nondestructive method to measure the demineralization depth of enamel whitespot lesions, which can be useful for the laboratory and has potential for clinical studies. Using the 1% sucrose and S mutans model for 6 days is a simple and effective method to induce enamel caries–like lesions without compromising the depth and morphologic features of the obtained lesions.e1-e6closedAccesstomographyoptical equipmentcoherent radiationoptical tomographypolarized beamsdentistrycariesbacteriaenamelsmicrobial processesstreptococcusstatistical dataArtigo de periódico1670000-0002-5018-9126https://orcid.org/0000-0002-5018-9126Sem Percentil26.00