SILVA, TAMIRESALVARENGA, LETICIALEAL, CINTIAMIRANDA, BIANCA G.SUZUKI, LUIZ C.KATO, ILKARIBEIRO, MARTHAPAVANI, CHRISTIANEPRATES, RENATO2017-10-172017-10-172017SILVA, TAMIRES; ALVARENGA, LETICIA; LEAL, CINTIA; MIRANDA, BIANCA G.; SUZUKI, LUIZ C.; KATO, ILKA; RIBEIRO, MARTHA; PAVANI, CHRISTIANE; PRATES, RENATO. Incorporation of the methylene blue photosensitizer in the presence of glucose in strains of candida albicans with multidrug efflux systems. <b>Lasers in Surgery and Medicine</b>, v. 49, n. 4, p. 438-438, 2017. Disponível em: http://repositorio.ipen.br/handle/123456789/27922.0196-8092http://repositorio.ipen.br/handle/123456789/27922Background: Infectious diseases are responsible for 60% of deaths in developing countries.Candida albicans cause severe infection in immunocompromised patients and the commonly used treatments have not been effective against microbial resistance. Photodynamic therapy (PDT) is a new promising strategy to microbial inactivation based on the use of photosensitizer (PS) in the presence of oxygen and activation by light to form reactive oxygen species.C.albicanshas glucose sensors which could facilitate the entrance of the PS. The objective of this study was to evaluate the role of glucose in the photodynamic inactivation of C. albicans. Study: C. albicans ATCC 10231, YEM 12, YEM 13, YEM 14, and YEM15were aerobically grown on Sabouraud agar and incubated at 308C for 24 hours. Microbial inoculants were divided into 4 groups with and without glucose: Control; Only irradiation; PS toxicity and PDT groups with three irradiation times.After going throughthe treatments, the colony-forming units were counted and the data were subjected to statistical analysis (ANOVA) and Tukey test. To measure the concentration ofMB, fluorescence spectroscopy and flow cytometry were used. Results: We observed that yeast with overexpression of Major Facilitator Superfamily membrane pore tend to accumulate more MB in its cytoplasm, whereas strains that overexpress ABC pumps demonstrated the greater survival against the photodynamic challenge. Conclusion: PDT is an efficient strategy against C. albicans and the presence of glucose can intervene in the photodynamic effect. The presence of MB in the intracellular region of the yeast predisposes to greater photodynamic inactivation, however, its germicidal capacity is related to the type and characteristics of the microbial.438-438openAccessResumos em periódicos449https://orcid.org/0000-0002-4203-1134