GANDARILLA, ARIAMNA M.D.GLORIA, JULIANE C.BARCELAY, YONNY R.SOUZA, RODRIGO F.B. deMARIUBA, LUIS A.M.BRITO, WALTER R.2022-12-012022-12-012022GANDARILLA, ARIAMNA M.D.; GLORIA, JULIANE C.; BARCELAY, YONNY R.; SOUZA, RODRIGO F.B. de; MARIUBA, LUIS A.M.; BRITO, WALTER R. Application of egg yolk IgY on carboxylated polypyrrole films for impedimetric detection of PfHRP2 antigen. <b>Bioelectrochemistry</b>, v. 148, p. 1-8, 2022. DOI: <a href="https://dx.doi.org/10.1016/j.bioelechem.2022.108273">10.1016/j.bioelechem.2022.108273</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/33374.1567-5394http://repositorio.ipen.br/handle/123456789/33374This paper described an impedimetric immunosensor for detecting Plasmodium falciparum histidine-rich protein 2 (PfHRP2). Antibodies from egg yolk (Ab-PfHRP2, IgY type) were linked covalently to the screen-printed gold electrodes (SPGE) surface modified with a thin film of Poly-pyrrole-pyrrole 3 carboxylic acid (P(Py-Py3COOH) to develop the sensing platform. The fabrication steps were followed by microscopic (scanning electron microscopy), spectroscopic (RAMAN spectroscopy and Energy-dispersive X-ray spectroscopy), and electrochemical (electrochemical impedance spectroscopy (EIS) and cyclic voltammetry) techniques. The determination of Ag-PfHRP2 was performed by EIS, and the BSA(bovine serum albumin)/Ab-PfHRP2(IgY)/P(Py-Py3COOH)/SPGE immunosensor recorded a linear response at 100–1000 ng mL−1 concentration range, with a limit of detection (LOD) of 27.47 ng mL−1. Its performance was confirmed by Enzyme-Linked Immuno-Sorbent Assay. The fabricated device uses a simple strategy of IgY immobilization, showing high sensitivity and good selectivity, and can be considered an alternative for carrying out malaria tests.1-8openAccessmalariaantibodiesimmunoglobulinsplasmodiumimmunoassaydetectionelectrodesArtigo de periódico14810.1016/j.bioelechem.2022.10827375.484