SABINO, C.P.RIBEIRO, M.S.BAPTISTA, M.S.LINCOPAN, N.2019-10-082019-10-08SABINO, C.P.; RIBEIRO, M.S.; BAPTISTA, M.S.; LINCOPAN, N. Antimicrobial photodynamic therapy challenges microbial drug-resistance. In: SÃO PAULO SCHOOL OF ADVANCED SCIENCE ON MODERN TOPICS IN BIOPHOTONICS, March 20-29, 2019, São Carlos, SP. <b>Abstract...</b> p. 61-61. Disponível em: http://repositorio.ipen.br/handle/123456789/30196.http://repositorio.ipen.br/handle/123456789/30196Background: The rising challenge of microbial resistance to chemotherapy demands immediate implementation of global policies and therapeutic alternatives. Antimicrobial photodynamic therapy (APDT) combines the administration of a photosensitizer (PS) compound with low-intensity monochromatic light to induce photochemical reactions that yield high amounts of reactive oxygen species (ROS). Since some PS molecular frameworks can be selectively incorporated by pathogens and ROS react with virtually all biomolecules, APDT offers a powerful strategy to challenge microbial resistance of local infections. Methods: In this study we assayed the APDT efficacy, using methylene blue (MB) as PS and red light provided by LED, against planktonic suspensions of high-risk representative fungal and bacterial species. The species tested include A. baumannii (OXA-23 and 143), E. aerogenes (NDM-1), E. faecalis (VAN-B), E. faecium (VAN-A), E. coli (MCR-1, CTX-M8 and 15), K. pneumoniae (KPC-2, IMP-1, OXA-48), S. aureus (MRSA, VISA), P. aeruginosa (VIM-1, SPM-1, GES- 5), C. albicans and C. neoformans. For all species, we tested standard control strains compared to azole-resistant yeast, or bacteria resistant to nearly all commercially available antimicrobials, in attempt to observe any crossresistance in between APDT and standard chemotherapy. Results: More than 5log10 reduction was observed within less than a minute of illumination for non-capsulated bacteria and within less than 5 minutes for yeast and capsulated bacteria. Regardless of resistance phenotype MB-APDT presented species-specific dose-response kinetics suggesting that similar therapeutic protocols may bring successful outcomes in clinical practice. Conclusions: Our study proposes that MB-APDT can efficiently inactivate a broad-spectrum of drug-resistant microorganisms and impair drug-resistance genes selection and dissemination.61-61openAccessResumo de eventos científicos0000-0002-4203-1134https://orcid.org/0000-0002-4203-1134